p-[4,5-dihydro-4-[[2-methoxy-5-methyl-4-[[2-(sulphooxy)ethyl]sulphonyl]phenyl]azo]-3-methyl-5-oxo-1H-pyrazol-1-yl]benzenesulphonic acid, sodium salt

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1991-01-09 to 1991-01-18

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Version / remarks:

1984

Qualifier:

according to guideline

Guideline:

EU Method C.1 (Acute Toxicity for Fish)

Version / remarks:

1984

Qualifier:

according to guideline

Guideline:

other: "Letale Wirkung beim Zebrabärbling Brachydanio rerio", Recommeded Test Guideline from the German Federal Environment Agency

GLP compliance:

yes

Analytical monitoring:

yes

Details on sampling:

- Concentrations: all
- Sampling method: 100 mL taken from the middle of the aquaria at 48 and 96 h

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: stirring of test item in dilution water (ISO/DIS 7346/1), homogenisation with Ultra-Turrax
- Controls: negative
- Evidence of undissolved material (e.g. precipitate, surface film, etc.): no

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name: zebrafish
- Source: West Aquarium, 3422 Bad Lauterberg, Germany
- Age at study initiation (mean and range, SD): mean: 102 d
- Length at study initiation (length definition, mean, range and SD): 2.6 - 3-3 cm, mean: 2.9 cm, SD: 0.27 cm

ACCLIMATION
- Acclimation period: min. 14 d
- Acclimation conditions (same as test or not): same
- Type and amount of food during acclimation: TetraMin, ad libitum
- Feeding frequency during acclimation: twice per day
- Health during acclimation (any mortality observed): no mortality

FEEDING DURING TEST
- Amount: No feeding

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Test temperature:

21.2 - 22.9°C

pH:

7.9 - 8.3

Dissolved oxygen:

8.0 - 9.6 mg/L

Nominal and measured concentrations:

Nominal: 0 (control), 500 mg/L
Measured: No measurements for control; 500 mg/L (0 h), 500 mg/L (48 h), 499 mg/L (96 h)

Details on test conditions:

TEST SYSTEM
- Test vessel: aquaria
- Material, size, headspace, fill volume: volume 10L, glass, 30 x 22 x 24 cm³
- Aeration: no
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Biomass loading rate: 1 fish/L

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: reconstituted water according to ISO/DIS 7346/1
- Culture medium different from test medium: no
- Intervals of water quality measurement: daily

OTHER TEST CONDITIONS
- Adjustment of pH: no, pH in dilution water was 8.2 - 8.3
- Photoperiod: 12/12
- Light intensity: 700 lux

EFFECT PARAMETERS MEASURED (with observation intervals if applicable): mortality, behaviour after 4, 24, 48, 72 and 96 h

TEST CONCENTRATIONS: limit test with 500 mg/L

Reference substance (positive control):

no

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 500 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: no mortality observed

Details on results:

- Behavioural abnormalities: reduced activity (all fish, 4 h); tail-orientated swimming (one fish, 4 h; some fish, 72 h); increased shock reaction (some fish, 72 h); observation was limited due to orange colour of test solution
- Mortality of control: no mortality
- Effect concentrations exceeding solubility of substance in test medium: no, solubility determined at >1000 mg/L

Validity criteria fulfilled:

yes

Conclusions:

No mortality was observed in a 96-h acute toxicity test with Danio rerio. The 96-h LC50 was determined >500 mg/L (analytically verified).

Description of key information

No mortality was observed in a 96-h acute toxicity test with Danio rerio. The 96-h LC50 was determined >500 mg/L (analytically verified). The results of the key study are the same as in an older supporting study performed with Leuciscus idus melanotus (96 -h LC50 > 500 mg/L).

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

500 mg/L