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Diss Factsheets
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EC number: 807-276-9 | CAS number: 1421695-64-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Some information in this page has been claimed confidential.
Administrative data
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 013
- Report date:
- 2013
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EPA OPPTS 850.1075 (Freshwater and Saltwater Fish Acute Toxicity Test)
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Details on test material:
- - Purity: 100%
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- Samples were collected from each test chamber at the beginning of the test and at 48 and 96 hours (± 1 hour) to measure concentrations of the test substance. Two sets of samples were collected at each interval. All samples were collected at mid-depth from the test chambers, placed in glass scintillation vials, and acidified with 2 drops of 10% phosphoric acid. One set of samples was processed immediately for analysis while the second set was stored refrigerated for possible future analysis.
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION:
Individual test solutions were prepared directly in each test chamber. A primary stock solution was prepared by mixing a calculated amount of test substance in 30 L of dilution water at a nominal concentration of 120 mg a.s./L, the highest concentration tested. The primary stock was prepared by mixing 3.6003 grams of test substance with a portion of the pre-measured dilution water in a 2 L glass flask, and sonicating the flask for approximately 5 minutes. The flask was poured into the appropriate aquaria and stirred with the remainder of the dilution water with a top-down electric mixer for approximately 1 hour. The stock solution was adjusted to 100% active ingredient during preparation based on the test substance purity (100%). At the end of mixing, the primary stock solution was clear and colourless with white foam on the surface. Aliquots of the primary stock solution were proportionally diluted with dilution water to prepare four additional test solutions at nominal concentrations of 7.5, 15, 30 and 60 mg a.s./L. The solutions were stirred with a top-down electric mixer for approximately 40 minutes. The 7.5, 15 and 30 mg a.s./L test solutions were clear and colourless with no visible precipitate. The 60 and 120 mg a.s./L test solutions appeared clear and colourless with a glossy surface. The negative control solution was dilution water only.
Test organisms
- Test organisms (species):
- Pimephales promelas
- Details on test organisms:
- TEST ORGANISM
- Age at study initiation: juveniles, The average total length of seven fish measured at the end of the test was 3.2 cm, with a range of 3.0 to 3.5 cm.
- Feeding during test: The fish were not fed for at least two days prior to the test or during the test.
ACCLIMATION PERIOD: at least 14 days
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
Test conditions
- Hardness:
- Hardness: 144 (mg/L as CaCO3)
- Test temperature:
- 21.9 - 22.3°C
- pH:
- 8.1 - 8.5
- Dissolved oxygen:
- 6.7 - 8.7 mg/L
- Salinity:
- freshwater
- Nominal and measured concentrations:
- Nominal concentrations: 0, 7.5, 15, 30, 60 and 120 mg a.s./L
Measured concentrations: 0, 8.9, 19, 35, 72 and 131 mg a.s./L - Details on test conditions:
- TEST SYSTEM
- Test vessel: 25-L stainless steel aquaria containing 15 L of test solution
- No. of organisms per vessel: 7
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
TEST MEDIUM / WATER PARAMETERS
Dissolved oxygen and pH were measured in each test chamber at the beginning and end of the test and at approximately 24 hours during the test. The test solutions were not aerated during the exposure period. When 100% mortality occurred in a test chamber, measurements of temperature, dissolved oxygen and pH were taken in that test chamber and then discontinued. Hardness, alkalinity and specific conductance were measured in the dilution water at the beginning of the test.
EFFECT PARAMETERS MEASURED: Observations were made periodically to determine the numbers of dead organisms in each treatment group. Lethality was defined as a lack of visible movement by the test organism. The numbers of individuals exhibiting sublethal effects or abnormal behaviour also were evaluated. Observations were made approximately 4.5, 24, 48, 72, and 96 hours after test initiation.
Results and discussion
Effect concentrations
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 50 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Details on results:
- Fathead minnows in the negative control group appeared normal throughout the test, with no observed mortality or sublethal effects. All fathead minnows in the 8.9, 19 and 35 mg a.s./L treatment groups also appeared normal throughout the test, with no mortality or overt signs of toxicity. Percent mortality at test termination in the 72 and 131 mg a.s./L treatment groups was 100%. The highest mean, measured test concentration causing no mortality at test end was 35 mg a.s./L. The lowest mean, measured test concentration causing 100% mortality at test end was 72 mg a.s./L.
- Reported statistics and error estimates:
- The mortality data were analysed using the computer program of C. E. Stephan. The program was designed to calculate the LC50 value and the 95% confidence interval by probit analysis, the moving average method, and binomial probability with nonlinear interpolation. In this study, nonlinear interpolation was used to calculate the 24, 48, 72 and 96-hour LC50 values and binominal probability was used to calculate the 95% confidence intervals. Due to the method used to calculate the 96-hour LC50 value, the slope of the dose response curve could not be calculated. The highest mean, measured test concentration causing no mortality at test end and the lowest mean, measured test concentration causing 100% mortality at test end were assessed by visual observation of the mortality and observation data.
Any other information on results incl. tables
- Sublethal observations / clinical signs:
Mean, Measured
Concentration (mg a.s./L)
Cumulative mortality (No. dead/No. at test start)
~4.5 hours
24 hours
48 hours
72 hours
96 hours
Water Control (0.0)
0/7
0/7
0/7
0/7
0/7
8.9
0/7
0/7
0/7
0/7
0/7
19
0/7
0/7
0/7
0/7
0/7
35
0/7
0/7
0/7
0/7
0/7
72
0/7a
4/7b
6/7c
7/7
7/7
131
0/7a
7/7
7/7
7/7
7/7
a7/7 fish lying at the bottom of the test chamber
b3/7 fish lying at the bottom of the test chamber
c1/7 fish lying at the bottom of the test chamber
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- 96-h LC50 = 50 mg a.s./L
- Executive summary:
The acute toxicity of the test substance to unfed juvenile fathead minnows, Pimephales promelas, a freshwater fish, was determined in an unaerated, static, 96-hour dose response test. The test was conducted in accordance with the OECD Guideline for Testing of Chemicals 203, and U.S. Environmental Protection Agency Series 850 - Ecological Effects Test Guidelines (draft) OPPTS Number 850.1075, Fish Acute Toxicity. Treatments consisted of a dilution water control and five nominal test concentrations of 7.5, 15, 30, 60 and 120 mg a.s./L (equivalent to 8.9, 19, 35, 72 and 131 mg a.s./L based on mean, measured concentrations). The 96-hour LC50 for Pimephales promelas was 50 mg a.s./L based on mean, measured test concentrations and mortality data. The highest mean, measured test concentration causing no mortality at test end was 35 mg a.s./L. The lowest mean, measured test concentration causing 100% mortality at test end was 72 mg a.s./L.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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