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The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
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EC number: - | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Skin irritation / corrosion
Administrative data
- Endpoint:
- skin irritation: in vitro / ex vivo
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 18 May 2011 - 15 November 2011
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 012
- Report date:
- 2011
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: OECD 439
- Deviations:
- no
- Principles of method if other than guideline:
- The objective of this study was to assess the skin irritation potential, in vitro, of the test substance. The test was conducted in accordance with the Standard Operating Procedure, In Vitro Skin Irritation Test: Human Epidermis Model (L’Oreal 2009), supplied by L’Oreal (leading laboratory in the validation of the test for ECVAM) and the OECD 439 Guideline For The Testing of Chemicals: In Vitro Skin Irritation: Reconstructed Human Epidermis Test Method (OECD439).
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- LZ1554
- IUPAC Name:
- LZ1554
- Details on test material:
- - Name of test material (as cited in study report): LZ1554
- Physical state: Highly viscous brownish liquid
- Expiration date of the lot/batch: 15 July 2012
- Storage condition of test material: Room temperature in the dark, under nitrogen
Constituent 1
Test animals
- Species:
- other:
- Strain:
- other:
Test system
- Controls:
- not required
- Amount / concentration applied:
- The positive and negative controls were in liquid form and were applied by dispensing a volume of 10 μl over each tissue using a positive displacement pipette. A weight of 10 ±2 mg of the test substance, LZ1554, a highly viscous brownish liquid, was applied to the tissue using the curved edge of a spatula. In order to spread 10 ±2 mg, a higher amount of 12 ±2 mg was weighed to compensate for some of the substance sticking to the spatula.
- Duration of treatment / exposure:
- After incubation of at least 24 hours in maintenance medium, triplicate tissues were dosed for 15 minutes with the test substance, negative or positive control at room temperature. A maximum of four samples were applied in a block with a minimum of 1 minute intervals between each application of substance. On application of 10 μl, the positive control was spread over the tissue for approximately 30 seconds and then respread with a curved flat spatula after approximately 7 minutes application time.
After 15 minutes, each tissue was rinsed with 25 ml sterile Dulbeccos Phosphate Buffered Saline (DPBS) to remove residual test substance. The surface of the tissues treated with the test substance, LZ1554, were also gently swabbed to remove residual test material. Inserts were then blotted on absorbent paper to remove remaining DPBS. Each insert was then transferred to a well containing 2 ml maintenance medium and incubated for 42 ± 1 hour at 37 ± 2°C in a humidified atmosphere of 5% CO2 in air.
After 42 ± 1 hour, each insert was transferred to a well containing 2 ml of 0.3 mg/ml MTT and incubated for 3 hours ± 5 minutes at 37 ± 2°C in a humidified atmosphere of 5% CO2 in air. - Observation period:
- At the end of 3 hours ± 5 minutes, the triplicate inserts were blotted on absorbent paper. The epidermis was removed from the insert using a biopsy punch, the epidermis separated from the collagen matrix using forceps and both parts placed in a micro tube. When all tissues had been punched, the tissues were vortexed with 500 El of acidic isopropanol (0.04 N HCl final concentration).
The tissues were extracted by storing at 2-8 ºC, protected from light, for 70 hours. - Number of animals:
- No animals were used as the method carried out was an in-vitro method.
Results and discussion
In vitro
Results
- Irritation / corrosion parameter:
- other: other: tissue viabiity
- Value:
- 107.8
- Remarks on result:
- other:
- Remarks:
- Basis: mean. Time point: 42 hours. Max. score: 111.9. (migrated information)
Applicant's summary and conclusion
- Interpretation of results:
- not irritating
- Remarks:
- Migrated information Criteria used for interpretation of results: EU
- Conclusions:
- It was concluded that the test substance, LZ1554, with a mean tissue viability of 107.8 ± 5.4%, was predicted as non-irritant to the skin.
- Executive summary:
The skin irritation/corrosion potential was determined in a GLP-compliant study in accordance with OECD guideline no. 439 (EPISKIN method). It was concluded that the test substance, LZ1554, with a mean tissue viability of 107.8 ± 5.4%, was predicted as non-irritant to the skin.
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