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Diss Factsheets
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EC number: 202-885-0 | CAS number: 100-74-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://echa.europa.eu/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_toxicological-information.png)
Toxicity to reproduction
Administrative data
- Endpoint:
- screening for reproductive / developmental toxicity
- Remarks:
- based on test type (migrated information)
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2003
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Justification for type of information:
- GLP study performed according to OECD Guideline 421. The original study report is in Japanese language, although the figures and tables are in English. An English summary is available from the Japanese authorities and an extensive summary is present in the OECD HPV program files.
Data source
Referenceopen allclose all
- Reference Type:
- other: public database
- Title:
- Unnamed
- Year:
- 2 003
- Reference Type:
- other: public database
- Title:
- Unnamed
- Year:
- 2 003
- Report date:
- 2003
- Reference Type:
- grey literature
- Title:
- Unnamed
- Year:
- 2 004
- Reference Type:
- publication
- Title:
- Toxicity to Reproduction
- Author:
- Japanese government
- Year:
- 2 005
- Bibliographic source:
- OECD SIDS Initial Assessment Report for SIAM 21 and OECD Agreed Conclusions
- Report date:
- 2007
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 421 (Reproduction / Developmental Toxicity Screening Test)
- Deviations:
- no
- GLP compliance:
- yes
- Limit test:
- no
Test material
- Reference substance name:
- 4-ethylmorpholine
- EC Number:
- 202-885-0
- EC Name:
- 4-ethylmorpholine
- Cas Number:
- 100-74-3
- Molecular formula:
- C6H13NO
- IUPAC Name:
- 4-ethylmorpholine
- Test material form:
- liquid
- Details on test material:
- - Alternative names of reference substance: Jeffcat NEM; N-ethylmorpholine; 4-ethylmorpholin; Morpholine, 4-ethyl-
- Molecular formula: C6H13NO
- Molecular weight: 115.17
- SMILES notation: CCN1CCOCC1
- InChl: InChI=1/C6H13NO/c1-2-7-3-5-8-6-4-7/h2-6H2,1H3
Constituent 1
- Specific details on test material used for the study:
- SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: 2901P0
STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: The test solution was kept in a refrigerator
- Stability under test conditions: The test solution was prepared and diluted to dosing concentrations by injection solvent every week. The diluted solution was confirmed to be stable for 8 days.
Test animals
- Species:
- rat
- Strain:
- other: Crj:CD(SD)IGS
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Crj:CD(SD)IGS rats bought from Charles River Japan, Inc
- Age at study initiation: 4 weeks old
- Weight at study initiation: no data
- Fasting period before study: no data
- Housing: no data
- Diet (e.g. ad libitum):no data
- Water (e.g. ad libitum):no data
- Acclimation period: 7 days
ENVIRONMENTAL CONDITIONS
- Temperature (°C): no data
- Humidity (%): no data
- Air changes (per hr): no data
- Photoperiod (hrs dark / hrs light): no data
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- water
- Details on exposure:
- Vehicle: water for injection.
The test solution was prepared and diluted to dosing concentrations by injection solvent every week. - Analytical verification of doses or concentrations:
- yes
- Details on analytical verification of doses or concentrations:
- The dosing solutions were confirmed to be stable.
- Duration of treatment / exposure:
- Males: 42 days
Females: from 14 days before mating to day 3 of lactation - Frequency of treatment:
- daily
Doses / concentrations
- Remarks:
- Doses / Concentrations:
0 (vehicle), 50, 150, 500 mg/kg bw/day
Basis:
nominal conc.
- No. of animals per sex per dose:
- 13
- Control animals:
- yes, concurrent vehicle
- Positive control:
- None.
Examinations
- Parental animals: Observations and examinations:
- CAGE SIDE OBSERVATIONS: Yes
DETAILED CLINICAL OBSERVATIONS: Yes
BODY WEIGHT: Yes, on day 1, 7, 14, 21, 28, 35, 42, 43 (males). Females: on day 1, 7, 14 pre-mating, on day 0, 7, 14, 20 of pregnancy and on day 0 and 4 of lactation.
FOOD CONSUMPTION: Yes, on days 1-2, 7-8, 14-15, 29-30, 35-36, 41-42 (males). Females on days 1-2, 7-8, 14-15 pre-mating, on days 0-1, 7-8, 14-15, 20-21 of pregnancy and on days 3-4 of lactation.
ORGAN WEIGHTS: Yes - Oestrous cyclicity (parental animals):
- mean length of estrous cycle during treatment period and pre-treatment period, number of animals showing 4-day cycle during pre-treatment period, changes of estrous cycle after treatment, mean times of vaginal estrus during mating period.
- Sperm parameters (parental animals):
- absolute and relative weights of testes and epididymides.
- Litter observations:
- STANDARDISATION OF LITTERS
No pups were discarded.
PARAMETERS EXAMINED
The following parameters were examined in F1 offspring:
number and sex of pups, stillbirths, live births, postnatal mortality, weight gain - Postmortem examinations (parental animals):
- Examined
- Postmortem examinations (offspring):
- Examined
- Statistics:
- Methods by Dunnett, bartlett, Fischer, Mann-Whitney
- Reproductive indices:
- copulation index, fertility index, gestation index, indexes for implantation, delivery
- Offspring viability indices:
- indexes for birth and live birth
Results and discussion
Results: P0 (first parental generation)
General toxicity (P0)
- Clinical signs:
- effects observed, treatment-related
- Body weight and weight changes:
- effects observed, treatment-related
- Food consumption and compound intake (if feeding study):
- effects observed, treatment-related
- Organ weight findings including organ / body weight ratios:
- no effects observed
- Histopathological findings: non-neoplastic:
- no effects observed
- Other effects:
- no effects observed
Reproductive function / performance (P0)
- Reproductive function: oestrous cycle:
- no effects observed
- Reproductive function: sperm measures:
- not examined
- Reproductive performance:
- no effects observed
Details on results (P0)
No deaths were found in males of any group. One female at 500 mg/kg bw/day died on day 2 of lactation. Tremor was observed in the female which died, and transient salivation after dosing was observed in males and females at 150 mg/kg bw/day and higher.
BODY WEIGHT AND FOOD CONSUMPTION (PARENTAL ANIMALS)
Decrease in body weight gain accompanied by reduced food consumption was detected in males at 500 mg/kg bw/day and females at 150 mg/kg bw/day and higher.
REPRODUCTIVE FUNCTION: ESTROUS CYCLE (PARENTAL ANIMALS)
No adverse effects on estrous cyclicity
REPRODUCTIVE FUNCTION: SPERM MEASURES (PARENTAL ANIMALS)
Not examined
REPRODUCTIVE PERFORMANCE (PARENTAL ANIMALS)
No effects on copulation index, fertility index, precoital interval, gestation length, gestation index and number of corpora lutea.
ORGAN WEIGHTS (PARENTAL ANIMALS)
Absolute and relative weights of the testes and epididymides in the group treated with this chemical were not different from the control group
GROSS PATHOLOGY (PARENTAL ANIMALS)
Necropsy revealed no changes related to the administration of this chemical.
HISTOPATHOLOGY (PARENTAL ANIMALS)
Histopathological examination of reproductive organs revealed no abnormalities related to dosing.
Effect levels (P0)
open allclose all
- Dose descriptor:
- NOAEL
- Effect level:
- 150 mg/kg bw/day (actual dose received)
- Based on:
- test mat.
- Sex:
- male
- Basis for effect level:
- other: Decreases in body weight gain and food consumption were detected in males of the 500 mg/kg group
- Dose descriptor:
- NOAEL
- Effect level:
- 50 mg/kg bw/day (actual dose received)
- Based on:
- test mat.
- Sex:
- female
- Basis for effect level:
- other: Decreases in body weight gain and food consumption were detected in females of the 150 and 500 mg/kg groups
Results: F1 generation
General toxicity (F1)
- Clinical signs:
- not specified
- Mortality / viability:
- mortality observed, treatment-related
- Body weight and weight changes:
- no effects observed
- Sexual maturation:
- not examined
- Organ weight findings including organ / body weight ratios:
- not examined
- Gross pathological findings:
- no effects observed
- Histopathological findings:
- not examined
Details on results (F1)
Effect levels (F1)
- Dose descriptor:
- NOAEL
- Generation:
- F1
- Effect level:
- 500 mg/kg bw/day (actual dose received)
- Based on:
- test mat.
- Sex:
- male/female
- Basis for effect level:
- other: No effects observed at highest dose tested.
Overall reproductive toxicity
- Reproductive effects observed:
- not specified
Applicant's summary and conclusion
- Conclusions:
- The NOAELs for general toxicity in parent animals are considered to be 150 mg/kg bw/day in males and 50 mg/kg bw/day in females, based on reduced body weight gain and food consumption. The NOAEL for reproductive and developmental toxicity is considered to be 500 mg/kg bw/day.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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