Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 276-649-0 | CAS number: 72403-66-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- From February 22 to June 03, 1994
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Qualifier:
- according to guideline
- Guideline:
- other: EEC Directive 84/449, L 251, Part C 2
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations:
NOMINAL
1.0/1.8/3.2/5.8/10/18/32/58 and 100 mg test article /l
MEASURED AT START
1.5/0.3/0.9/3.4/8.2/4.4/4.7/11.4/28.2 mg test article /l
MEASURED AT END
1.0/2.2/2.7/5.4/11.8/6.5/6.2/13.0/28.2 mg test article /l
- Sampling method: All test medium samples for the analytical determinations were taken from the approximate center of the test vessels (without mixing the medium); the concentrations of the test substance at the beginning and at the end of the test was analysed by spectrophotometry (459nm).
- Sample storage conditions before analysis: kept at -18°C to -25°C until analyses - Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: 0.5 g test substance were mixed with 200 ml test medium and fill up to 500 ml with test medium. The stock solution was homogenized for 20 minutes in the ultrasonics bath. - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Strain: Daphnia magna
- Source: bred in the testing facility of the Product Ecology TF, Textile Dyes Division under standardised conditions
- Method of breeding: Cultures of Daphnia magna are maintained in glass vessels containing aprox. 3.0 l of reconstituted water at 18 - 22°C. The water was renewed partially three times weekly. The Daphnia were feed with a suspension of green algae (Scenedesmus subspicatus) supplemented by a suspension of TETRAMIN-extract in such quantites that the food was consumed within 24h - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Hardness:
- 136 mg/l CaCO3
- Test temperature:
- 21 ± 1 °C measured at the beginning and at the end of the test
- pH:
- 7.8 - 8.2
- Dissolved oxygen:
- 98 - 99 %
- Nominal and measured concentrations:
- NOMINAL
1.0/1.8/3.2/5.8/10/18/32/58 and 100 mg test article /l
MEASURED AT START
1.5/0.3/0.9/3.4/8.2/4.4/4.7/11.4/28.2 mg test article /l
MEASURED AT END
1.0/2.2/2.7/5.4/11.8/6.5/6.2/13.0/28.2 mg test article /l - Details on test conditions:
- TEST SYSTEM
- Test vessel: 250ml beakers
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 2
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: bidistilled water with these components:
NaHCO3 65 mg/l
MgSO4 x 7 H20 123.3 mg/l
KCI 5.8 mg/l
CaCI2 x 2 H20 294 mg/l
Na2SiO3 x 9 H20 10 mg/l
NaNO3 274 µg/l
KH2PO4 143 µg/l
K2HPO4 184 µg/l
- Alkalinity: 0.8 mmol/l
- Ca/mg ratio: 4:1
- Culture medium different from test medium: no
OTHER TEST CONDITIONS
- Photoperiod: 16 hours light/8 hours dark
TEST CONCENTRATIONS
- Spacing factor for test concentrations: ca. 1.8
- Test concentrations: 1.0/1.8/3.2/5.8/10/18/32/58 and 100 mg test article /l (nominal) - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate
- Duration:
- 24 h
- Dose descriptor:
- EC0
- Effect conc.:
- >= 1.3 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: actual concentration measured by analytical analysis
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- ca. 5 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: The EC50 value are calculated with the " Probit model " method; CL 95%: 3.78 - 6.51
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- ca. 4 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: EC50 value graphically determined.
- Duration:
- 24 h
- Dose descriptor:
- EC100
- Effect conc.:
- > 28.2 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: actual concentration measured by analytical analysis
- Duration:
- 48 h
- Dose descriptor:
- EC0
- Effect conc.:
- >= 1.3 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: actual concentration measured by analytical analysis
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- ca. 2.3 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: The EC50 value are calculated with the " Probit model " method; CL 95%: 1.83 - 2.71
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- ca. 1.6 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: EC50 value graphically determined.
- Duration:
- 48 h
- Dose descriptor:
- EC100
- Effect conc.:
- ca. 12.2 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: actual concentration measured by analytical analysis
- Details on results:
- - Mortality of control: in the control no immobilization of the Daphnia was observed.
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: in the test concentrations from nominal 18 mg/l up to 100 mg/l a sedimentation in the test beakers was observed after 48 hours. - Results with reference substance (positive control):
- - Results with reference substance valid, according to the results of the inter laboratory tests and a Technical Corrigendum to ISO 6341 give an EC50-24 h of the potassium dichromate (K2Cr2O7) within the range 0.6 mg/l to 2.1 mg/l. Reference: OECD guideline 202.
- EC50: 1.01 mg/l
The quality of the Daphnia was checked at least every 3 months by determining the EC50 values (24 h) for potassium dichromate. - Validity criteria fulfilled:
- yes
- Conclusions:
- EC50 (48h) = 2.3 mg/l
- Executive summary:
Method
The test has been conducted in agreement with OECD guideline 202 and EEC Directive 84/449, L 251, Part C 2.
The test was performed in compliance with the Good Laboratory Practice Regulations.
The substance was tested for its acute toxicity to Daphnia magna in a 48 hours static test. 24 hours before the start of the exposure reproductive Daphnia were separated from the young Daphnia by sieving all individuals through a 710 µm sieve. For each concentration, of test substance, 20 Daphnia divided in two groups of 10 animals were used.
The Daphnia were exposed to the test article in 250 ml beakers containing 100 - 200 ml test medium with 10 animals per beaker. During exposure the animals were kept at 21 ± 1 °C with 16 hours illumination. The oxygen concentration, the pH and the temperature were controlled at the beginning and at the end of the test.
Based on a pretest, the test concentrations were chosen as 1.0/1.8/3.2/5.8/10/18/32/58 and 100 mg test article/l (nominal).
All test medium samples for the analytical determinations were taken from the approximate center of the test vessels (without mixing the medium) and kept at -18 °C to -25 °C until analysis.
Test evaluations were done according to the guidelines by visual assessment of mobility of the Daphnia after 24 and 48 hours.
Results
The EC50 was determined in two ways:
- graphically: EC50 4 mg/l (24h); EC50 1.6 mg/l (48h)
- statistically (probit): EC50 5 mg/l (24h); EC50 2.3 mg/l (48h)
Reference
Description of key information
EC50 = 2.3 mg/l.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 2.3 mg/L
Additional information
To determine the short-term toxicity to aquatic invertebrates, the substance under registration was tested on Daphnia sp. according to OECD Guideline 202. The concentrations tested were: 1.5/0.3/0.9/3.4/8.2/4.4/4.7/11.4/28.2 mg test article /l based on analytical results. The nominal concentrations were 1.0/1.8/3.2/5.8/10/18/32/58 and 100 mg test article /l.
The EC50 was determined in two ways: graphically, interpolating the point plotted on a graph; and statistically, calculating with the "probit model" method. The EC50 at 48 hours were 1.6 and 2.3 mg/l respectively. The EC50 value obtained statistically has been used as reference due to the more reliability of the method.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.