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EC number: 205-759-3 | CAS number: 150-39-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Eye irritation
Administrative data
- Endpoint:
- eye irritation: in vivo
- Type of information:
- migrated information: read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Study period:
- 18 May 1982 - 8 June 1982
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Meets generally accepted scientific standards, documentation is sufficient for assessment
Cross-reference
- Reason / purpose for cross-reference:
- reference to same study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 982
- Report date:
- 1982
Materials and methods
- Principles of method if other than guideline:
- This study followed internal Dow procedures
- GLP compliance:
- not specified
Test material
- Reference substance name:
- Trisodium 2-(carboxylatomethyl(2-hydroxyethyl)amino)ethyliminodi(acetate)
- EC Number:
- 205-381-9
- EC Name:
- Trisodium 2-(carboxylatomethyl(2-hydroxyethyl)amino)ethyliminodi(acetate)
- Cas Number:
- 139-89-9
- Molecular formula:
- C10H18N2O7.3Na
- IUPAC Name:
- trisodium [{2-[bis(carboxylatomethyl)amino]ethyl}(2-hydroxyethyl)amino]acetate
- Details on test material:
- - Name of test material (as cited in study report): VERSENOL * 120 + 1.9% EXCESS SODIUM HYDROXIDE, also known as hydroxyethyl ethylene diamine triacetate, trisodium salt
- Physical state: liquid
- Source: Larkin Laboratory, Midland, Michigan, USA
- Other: pale yellow color
Constituent 1
Test animals / tissue source
- Species:
- rabbit
- Strain:
- New Zealand White
- Details on test animals or tissues and environmental conditions:
- TEST ANIMALS
- Source: Langshaw Farms, Augusta, Michigan, USA
- Other: Female
- All other template details: Not reported
ENVIRONMENTAL CONDITIONS - Not reported
IN-LIFE DATES: Not reported
Test system
- Vehicle:
- other: VERSENOL * 120 + 1.9% EXCESS SODIUM HYDROXIDE
- Controls:
- no
- Amount / concentration applied:
- ~0.1 mL or 0.1 g
- Duration of treatment / exposure:
- Washed and unwashed - Test material of ~ 0.1 mL or 0.1 g was placed in the conjunctival sac of the eyes.
Washed - Following a 30 seconds exposure period the right eye was washed for 2 minutes with tepid, flowing water.
Unwashed. The test material was instilled in the left eye which was left unwashed. - Observation period (in vivo):
- At various time intervals both eyes were examined for conjunctival irritation, corneal injury and irritation to the iris. The behavior of the animal was observed for indication of pain or discomfort. A drop of 5% fluorescein was used to help assess corneal injury at 1-hour reading and all subsequent readings.
immediate
after 1hour
after 24 hours
after 48 hours
after 7 days
after 14 days
after 21 days - Number of animals or in vitro replicates:
- One
- Details on study design:
- REMOVAL OF TEST SUBSTANCE
- Washing (if done): yes with tepid flowing tap water to right eye for 2 minutes
- Time after start of exposure: 30 seconds
SCORING SYSTEM: see table in results
TOOL USED TO ASSESS SCORE: 5% aqueous fluorescein
Results and discussion
In vivo
Results
- Irritation parameter:
- other: combination of conjunctiva, cornea, iris
- Basis:
- animal #1
- Time point:
- other: 21 days
- Score:
- >= 1 - <= 6
- Max. score:
- 6
- Reversibility:
- fully reversible within: 14 days if washed out after 30 seconds
- Remarks on result:
- other: see Table 1
- Irritant / corrosive response data:
- See Table 1
- Other effects:
- Not reported
Any other information on results incl. tables
Table 1. Eye irritation test results.
immediate
response not washed (left) washed (right)
pain * 6
conjunctiva 3 3
cornea 2 2
iris 4 4
* - anesthetized eye
1 hour
response not washed (left) washed (right)
conjunctiva 4 4
cornea (before stain) 4 3
cornea (after stain) 4 4
iris 3 5
24 hour
response not washed (left) washed (right)
conjunctiva 4 4
cornea (before stain) 4 3
cornea (after stain) 4 4
iris 4 4
48 hour
response not washed (left) washed (right)
conjunctiva 4 4
cornea (before stain) 4 2
cornea (after stain) 4 4
iris 4 4
7 days
response not washed (left) washed (right)
conjunctiva 2 2
cornea (before stain) 6 * 1
cornea (after stain) 3 1
iris 4 3
* Cornea 95% opaque, vascularization on top area of cornea. Right cornea appears to be ulcerated.
14 days
response not washed (left) washed (right)
conjunctiva 2 2
cornea (before stain) 6 * 1
cornea (after stain) 2 1
iris @ 1
* - Cornea has vascularization
@ - Iris cannot be evaluated
21 days
response not washed (left) washed (right)
conjunctiva 2 1
cornea (before stain) 5 * 1
cornea (after stain) 2 1
iris 1 1
* - cornea has vasularization and the area of opacity is < 50% of cornea
KEY
Pain
1 - No response.
2 - Very slight. No more than a few blinks - Normal in a minute or so.
3 - Slight. Blinks - tries to open but can't. Reflexes close eye.
4 - Moderate. Holds eye shut and puts pressure on lids, may rub eye with paw.
5 - Severe. Holds eye shut vigorously, rubs eye with paw - may squeal.
6 - Very severe. Holds eye shut vigorously. May squeal, claws at eye, jumps, tries to escape.
Conjunctival response
1 - None.
2 - Very slight. Capillaries prominent. Very slight swelling and/or redness.
3 - Slight. Conjunctival membranes somewhat inflamed. May be sclero-corneal inflammation. Similar to response from a mild soap.
4 - Moderate. Conjunctival membranes inflamed and sore, lids swollen and reddened.
5 - Severe. Conjunctival membranes red and swollen. Nictitating membrane sore and inflamed.
6 - Very Severe. Conjunctival membranes markedly reddened and swollen. Nictitating membrane edematous. Exudate contains pus - eye may be suck shut.
Corneal Response (before Staining)
1 - No response
2 - Very slight haziness.
3 - Slight. Cloudy in parts.
4 - Moderate. Cloudy over whole but not opaque.
5 - Severe. Cornea up to 50% opaque.
6 - Very severe. Cornea completely opaque.
Corneal Response (after staining)
1 - None.
2 - Very slight haziness. Few areas of epithelial injury. Probably heal in 24 - 48 hours.
3 - Slight. Up to 50% of eye covered with shallow epithelial injury. Probably heals.
4 - Moderate. 50 - 100% of eye injured but lesions are shallow. Doubtful if vision will be impaired.
5 - Severe. Marked injury over whole area which may result in loss of eye.
6 - Very severe. Eye lost.
Irritation of the iris
1 - No response.
2 - Very Slight.
3 - Slight. Just perceptible.
4 - Moderate. Definitely perceptible
5 - Severe. Marked change.
6 - Very severe. Complete loss of function.
Applicant's summary and conclusion
- Interpretation of results:
- Category 1 (irreversible effects on the eye)
- Remarks:
- Migrated information Criteria used for interpretation of results: EU
- Conclusions:
- Installation of Versenol 120 + 1.9% excess sodium hydroxide into the unwashed eye of a female New Zealand white rabbit resulted in moderate conjunctival redness and swelling, moderate reddening of the iris and corneal opacity. Signs of irritation were still exhibited 21 days following exposure. When sample was washed after exposure for 30 seconds, signs of irritation persisted until 14 days post-exposure.
- Executive summary:
A sample of Versenol 120 + 1.9% excess sodium hydroxide also known as hydroxyethyl ethylene diamine triacetate, trisodium salt was submitted for assessment of eye irritation potential. The eye irritation test was conducted on a female New Zealand white rabbit.
Instillation of this undiluted material into the rabbit eye which was subsequently washed resulted in marked discomfort, moderate conjunctival redness and swelling, marked reddening of the iris and moderate corneal cloudiness. All signs of irritation were essentially absent 14 days post-exposure.
Instillation of this undiluted material into the unwashed eye of the rabbit resulted in moderate conjunctival reddened and swelling, moderate reddening of the iris and corneal opacity. Signs of irritation were still exhibited 21 days following exposure. Chemical workers' goggles are recommended whenever the likelihood of human eye contact exists, as permanent impairment of vision may result. If contact does occur, contaminated eyes must be flushed immediately with copious amounts of water. Medical attention should be obtained.
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