3-phenoxybenzylic alcohol

Genetic toxicity: in vitro

Administrative data

Endpoint:

in vitro gene mutation study in bacteria

Remarks:

Type of genotoxicity: gene mutation

Type of information:

experimental study

Adequacy of study:

weight of evidence

Reliability:

4 (not assignable)

Rationale for reliability incl. deficiencies:

other: Data is from online Chemical Repository Database

Data source

Reference

Reference Type:

publication

Title:

Unnamed

Year:

1993

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other:

Principles of method if other than guideline:

Salmonella/microsomal assay to investigate the mutagenic potentlaI of a test compound 3-Phenoxybenzyl alcohol (3-PBA)

GLP compliance:

not specified

Type of assay:

bacterial gene mutation assay

Test material

Test material information

Constituent 1

Reference substance name:

3-phenoxybenzylic alcohol

EC Number:

237-525-1

EC Name:

3-phenoxybenzylic alcohol

Cas Number:

13826-35-2

Molecular formula:

C13H12O2

IUPAC Name:

(3-phenoxyphenyl)methanol

Test material form:

other: liquid

Details on test material:

- Name of test material (as cited in study report): 3-Phenoxybenzyl alcohol
- Molecular formula (if other than submission substance): C13H12O2
- Molecular weight (if other than submission substance): 200.23318 g/mol
- Smiles notation (if other than submission substance): C1=CC=C(C=C1) OC2=CC=CC (=C2) CO
- InChl (if other than submission substance): InChI=1S/C13H12O2/c14-10-11-5-4-8-13(9-11)15-12-6-2-1-3-7-12/h1-9, 14H, 10H2
- Substance type: Organic
- Physical state: liquid

Method

Species / strain

Species / strain / cell type:

S. typhimurium, other: TA1535, TA100, TA1537, TA1538 and TA98

Details on mammalian cell type (if applicable):

- Type and identity of media: Spizzizen's minimal medium ,Noble's agar,Nutrient broth

Additional strain / cell type characteristics:

not specified

Metabolic activation:

with and without

Metabolic activation system:

S-9 from Sprague-Dawley rats

Vehicle / solvent:

- Vehicle(s)/solvent(s) used: DMSO

Controls

Untreated negative controls:

yes

Negative solvent / vehicle controls:

yes

True negative controls:

not specified

Positive controls:

yes

Positive control substance:

9-aminoacridine

2-nitrofluorene

other: N-methyl- N -nitro-N-nitrosoguanidine,2-Aminoanthracene,2-Aminofluorene, 6-Aminochrysene, Aflatoxin B1

Details on test system and experimental conditions:

METHOD OF APPLICATION :in agar (plate incorporation)

Evaluation criteria:

significant increase in the number of revertant mutant colonies when compared to the spontaneous background rate which occurs in the control cultures

Statistics:

Linear regression analysis to determine strength of dose response

Results and discussion

Test results

Species / strain:

S. typhimurium, other: TA1535, TA100, TA1537, TA1538 and TA98

Metabolic activation:

with and without

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

not specified

Vehicle controls validity:

not specified

Untreated negative controls validity:

not specified

Positive controls validity:

not specified

Remarks on result:

other: all strains/cell types tested

Remarks:

Migrated from field 'Test system'.

Any other information on results incl. tables

RESULTS OF SALMONELLA/MICROSOMAL ASSAY WITHOUT METABOLIC ACTIVATION ON 3-PBA

compound	conc units/ plate	REVERTANTS PER PLATE OF BACTERIAL TESTER STRAIN TA1535           TA1537             TA1538              TA100              TA98
bacteria only (negative control)	100.0000 µL	7.0  ± 2.0	5.3  ± 1.2	9.7  ± 1.5	126.7  ±   10.4	15.3  ±   5.5
DMSO (SOLVENT CONTROL)	100.0000 µL	9.0  ± 2.6	5.3  ± 1.5	8.7  ± 3.5	139.0  ±   24.2	16.0  ±   1.0
MNNG (POSITIVE CONTROL)	5.0000 µg	813.7   ± 66.2	NT	NT	855.3  ± 203.3	NT
DMSO (POSITIVE CONTROL)	100.0000 µg	NT	463.3  ±   62.4	NT	NT	NT
DMSO (POSITIVE CONTROL)	5.000 µg	NT	NT	264.3  ± 110.6	NT	271.3  ±    40.4
3-PBA	.0050 µL	11.3  ±   2.1	5.0  ± 2.0	4.0  ± 1.0	229.3  ±   29.3	15.0  ±   3.5
0.5 % SOLUTION (v/v) IN DMSO	.0100 µL	16.3  ±   3.2	3.7  ± 2.1	4.0  ± 1.0	187.7  ±   18.6	15.3  ±   4.5
	.050 0 µL	16.7  ±   2.5	6.0  ± 2.6	6.7  ± 3.1	201.7  ±   26.8	16.3  ±   2.5
	.1000 µL	13.7  ±    1.5	6.3  ±   .6	3.3  ± 2.5	189.7  ±     7.8	17.7  ±    2.3
	.5000 µL	10.3  ±   1.5	1.7  ± 0.6	6.0  ±   .0	155.3  ±   21.4	17.7  ±    2.1

NT: not tested

 

RESULTS OF SALMONELLA/MICROSOMAL ASSAY WITH METABOLIC ACTIVATION ON 3-PBA

compound	conc units/ plate	Revertants per plate of bacterial tester strain TA1535           TA1537             TA1538              TA100              TA98
Bacteria only (negative control)	100.0000 µL	7.0  ± 2.0	5.3  ± 1.2	9.7  ± 1.5	126.7  ±   10.4	15.3  ±   5.5
S-9 FRACTION(NEGATIVE CONTRL)	100.0000 µL	8.3  ±  .6	5.3  ± 0.6	9.3  ± 2.3	131.7  ±   41.5	21.7  ±   3.1
DMSO( SOLVENT CONTRL)	100.0000 µL	9.7   ±  2.5	6.3  ±  1.2	11.3  ±  3.8	126.3  ±   38.1	17.3 ±    8.4
2-AA(POSITIVE CONTROL)	5.0000 µg	74.0   ±  10.1	NT	NT	NT	NT
6-AC(POSITIVE CONTROL)	1.000 µg	NT	51.3  ±    7.1	NT	NT	NT
2-AF(POSITIVE CONTROL)	2.000 µg	NT	NT	256.7  ±   64.5	NT	NT
AFLTOXIN (POSITIVE CONTROL)	1.000 µg	NT	NT	NT	656.3  ± 203.9	307.0  ±   66.8
3-PBA 0.5 % SOLUTION (V/V) IN DMSO	.0050 0 µL	14.7  ±   2.3	13.3  ±   2.1	18.7  ±   5.0	280.3  ±   18.6	18.7  ±   5.0
	.01000 µL	8.3  ±  1.5	16.7  ±   4.0	13.0  ±   1.0	245.7  ±     4.5	22.3  ±    8.5
	.05000 µL	7.0  ±  0.0	12.0  ±   2.6	15.3  ±   1.5	243.7  ±   16.8	23.3  ±    3.5
	.1000 µL	12.0  ±  4.6	13.3  ±    5.5	11.7  ±   2.5	216.0  ±   10.4	20.0  ±   7.5
	.5000 µL	12.3  ± 4.2	10.0  ±  1.0	5.7  ±   2.1	175.0  ±  39.9	12.0  ±   2.6

NT: not tested

Applicant's summary and conclusion

Conclusions:

Interpretation of results (migrated information):
negative

The genetic toxicity test for 3-Phenoxybenzylalcohol (CAS No: 13826-35-2) on salmonella strains TA1535, TA100, TA1537, TA1538 and TA98 was found to be negative with and without activation.

Executive summary:

Genetic toxicity test was performed by using 3-Phenoxybenzylalcohol onsalmonella strainsTA1535, TA100, TA1537, TA1538 and TA98 with and without metabolic activation.Liver S-9 preparations were done from Sprague-Dawley male rats pretreated with Aroclor 1254 for the non-specific induction of hepatic enzymes served as the activation system. S-9 preparation was used at a final concentration of 100 µl per ml of S-9 mix. Dimethyl sulphoxide (DMSO) was the solvent used. Spizzizen's minimal medium, Noble's agar, Nutrient broth were the medium used in these experiments.

 

The test was performed according to the method of Ames et al.For the assay, the following were added, in order to 16 x 125 mm screw-capped tubes:

1.2 ml molten agar (0.6% Noble's agar supplemented with 0.6% NaCl , 0.5 mM biotin, and 0.5 mM histidine).

2.0.1 ml of bacterial culture.

3.0.1 ml of test agent in the appropriate solvent.

Tubes were mixed quickly but gently and the contents poured over a base plate of Spizzizen's minimal medium. Plates were rotated to distribute the overlay agar, allowed to harden and incubated at 37⁰C for 48 hr at which time the number of mutant colonies were determined.

In case of metabolic activation with S-9, all procedure was same except that 0.5 ml of S-9 mix was added to the overlay agar immediately before it was mixed and poured. Plates were incubated.

 

Each agent was tested at five concentrations against all five strains of bacteria.

The following controls were included in each experiment:

Bacteria only for spontaneous reversion (Negative control), Bacteria and solvent for solvent control.Chemical used as positive control:Without metabolic activation areN-methyl- N -nitro-N-nitrosoguanidine, 2-Nitrofluorene, 9-Aminoacridineandwith metabolic activation:2-Aminoanthracene, 2-Aminofluorene, 6-Aminochrysene, Aflatoxin B_1.   

 

The test agent did not induce a significant increase in the number of point mutations inSalmonella typhimuriumstrains in the absence and in the presence of the activating system for strains TA1535, TA1537, TA1538, TA100, and TA98.

 

So from the above test procedure it was concluded thatthe genetic toxicity test for 3-Phenoxybenzylalcohol (CAS No: 13826-35-2) onsalmonellastrains TA1535, TA100, TA1537, TA1538 and TA98 was found to be negative with and without activation.