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EC number: 205-503-0 | CAS number: 141-82-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2001
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- data from handbook or collection of data
- Justification for type of information:
- Succinic acid may be used as an analogue for malonic acid, based on the following considerations. Firstly, dicarboxylic acids are naturally occurring metabolic products of fatty acid oxidation, and are rapidly beta-oxidised. A category approach for short-medium chain dicarboxylic acids, including malonic acid and succinic acid, has been validated and used by various bodies including the Cosmetics Ingredient Review Panel and the European Food Safety Authority.Disodium succinate may be used as an analogue for succinic acid as there is no significant difference in toxicity expected between succinic acid and disodium succinate, since both substances will dissociate into succinate ion under physiological conditions.
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- GLP compliance:
- yes
- Specific details on test material used for the study:
- Disodium succinate hexahydrate (CAS No.: 6106-21-4, Nakarai Kagaku, Inc. (Japan), Lot. No.: MOT9476, Purity = 100.2%
- Analytical monitoring:
- yes
- Details on test solutions:
- Test chemical was refrigerated. The stability of the chemical was confirmed by IR spectrum. The IR spectrum at the end of the test was same at the start of test.
- Test organisms (species):
- Selenastrum sp.
- Details on test organisms:
- Species : Selenastrum capricornutum (Algae)Supplier/Source: Obtained from American Type Culture Collection and reproduced in aseptic cultureMethod of Cultivation: SterileStain Number: ATCC22662Pre-culture (duration, medium, etc.): Test alga was pre-incubated for 3 days under the same method of test in OECD medium
- Test type:
- static
- Water media type:
- freshwater
- Total exposure duration:
- 72 h
- Test temperature:
- water temperature: 23.0 - 23.6ºC
- pH:
- pH: 7.7 - 7.9 (at the start of the test)10.3 - 10.5 (at the end of the test)
- Details on test conditions:
- Medium: OECD mediumExposure Vessel Type: 100 mL Medium in a 500mL Erlenmeyer Flaskwith silicon cap (open system)Vehicle/Solvent and Concentrations: Any solvent was not used.Number of Replicates: 3Initial Cell Number: 10,000 cells/mLLight Condition: 4,000 - 5,000 lux, continuouslyShaking: 100 rpm
- Reference substance (positive control):
- yes
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 998 mg/L
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- > 998 mg/L
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 998 mg/L
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- > 998 mg/L
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Results with reference substance (positive control):
- EbC50 (0-72 hr) for a reference substance (potassium dichromate) was 0.427 mg/L.
- Reported statistics and error estimates:
- Data Analysis: The calculated inhibition rate at the highest concentration based on growth rate inhibition and biomass were less than 50%, therefore the EC50 was more than the highest concentration. The NOEC values were determined by analysis of variance (ANOVA).Method of Calculating Mean Measured Concentrations (i.e. arithmetic mean, geometric mean, etc.): time-weighted mean
- Validity criteria fulfilled:
- yes
- Conclusions:
- In an algal growth inhibition test (OECD TG 201, open system), the acute toxicity results (72 h ErC50 and 72 h EbC50) to Selenastrum capricornutum were were determined to be >998 mg/L by both the biomass method and the growth rate method. Disodium succinate was considered to be not toxic to algae according to EU classification criteria.
- Executive summary:
In an algal growth inhibition test (OECD TG 201, open system), the acute toxicity results (72 h ErC50 and 72 h EbC50) to Selenastrum capricornutum were were determined to be >998 mg/L by both the biomass method and the growth rate method. Disodium succinate was considered to be not toxic to algae according to EU classification criteria.
Reference
Percent Growth Inhibition of Selenastrum capricornutum
Measured Conc mg/L | Area under the growth curves (Average) | |
Area A (0-72hr) | Inhibition (%)* IA (0-72hr) | |
Control | 25,400,000 | --- |
247 | 27,900,000 | -10.2 |
501 | 28,600,000 | -12.8 |
998 | 28,300,000 | -11.7 |
Mean Measured Conc. mg/L | Growth rates and percent inhibition (Average) | |||
Rate u(24-48hr) | Inhibition(%) Im(24-48hr) | Rate u(0-72hr) | Inhibition(%) Im(0-72hr) | |
Control | 0.0771 | --- | 0.0665 | --- |
247 | 0.0778 | -0.875 | 0.0642 | 3.37 |
501 | 0.0779 | -0.954 | 0.0650 | 2.21 |
998 | 0.0771 | -0.138 | 0.0660 | 0.745 |
Growth Curves: During the test period algae grew almost linearly(log scale) in each concentration.
Measured Concentrations: The tested concentrations were measured at the start and the 72nd hour. For all of them, the deviations from the nominal concentration were less than +/-20%.
Nominal conc. mg/L | Measured Conc., mg/L | Mean* mg/L | Percent of nominal | ||
0 Hour | 72 Hour |
| 0 Hour | 72 Hour | |
Control | <15.0 | <15.0 | --- | --- | --- |
250 | 249 | 246 | 247 | 99.7 | 98.3 |
500 | 504 | 498 | 501 | 101 | 99.6 |
1,000 | 1,000 | 997 | 998 | 100 | 99.7 |
*: Mean measured concentration (time-weighted mean)
Description of key information
In an algal growth inhibition test (OECD TG 201, open system), the acute toxicity results (72 h ErC50 and 72 h EbC50) to Selenastrum capricornutum were were determined to be >998 mg/L by both the biomass method and the growth rate method. Disodium succinate was considered to be not toxic to algae according to EU classification criteria.
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 998 mg/L
- EC10 or NOEC for freshwater algae:
- 998 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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