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EC number: 200-270-1 | CAS number: 56-37-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 19/07/2016 - 23/07/2017
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study
- Justification for type of information:
- Experimental test result performed using standard OECD test guidelines
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Principles of method if other than guideline:
- This study was designed to assess the toxic effects of the test compound on the Zebra Fish Danio rerio .Bowl aquaria containing 2 liters of potable water (passed through reverse osmosis system) were loaded with 8 fishes. A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203.
- GLP compliance:
- yes
- Analytical monitoring:
- not specified
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
The test substance was soluble in water. Therefore, the test solution was prepared by dissolving 400 mg of the test substance in 1 liters of potable water (passed through reverse osmosis system) with continuous stirring. This test solution was then added to the remaining three liters of water for achieving test concentrations of 100 mg/L. - Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- TEST ORGANISM
- Common name:Zebra Fish
- Source:Unique Aqua Fish Corner
- Age at study initiation (mean and range, SD):No data
- Length at study initiation (length definition, mean, range and SD):average 3.42 cm
- Weight at study initiation (mean and range, SD):average 0.335 g
- Method of breeding:The fishes were kept in a static tank in tap water passed through reverse osmosis system, under natural conditions along with proper feed and aeration.
- Food type:Standard brand feed
- Frequency:once daily
ACCLIMATION
- Acclimation period:7 days
- Acclimation conditions (same as test or not):Fishes, to be used in the study, are kept in the test water for seven days immediately before testing
- Type and amount of food:Standard brand feed
- Feeding frequency:Once in a day up to sixth day of acclimatization
- Health during acclimation (any mortality observed):No mortality
HOUSING:
-Duration of housing:15 days
-Photoperiod-12 hours light and 12 hours darkness
-Oxygen content-7.06 (mg/l)
QUARANTINE (wild caught)
- Duration:96 hrs
- Health/mortality:During last 48 hours = 0 % - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 96 h
- Hardness:
- 140.5(mg of CaCO3)
- Test temperature:
- 25°C
- pH:
- 7.14
- Dissolved oxygen:
- 6.66 mg/l
- Salinity:
- No data
- Nominal and measured concentrations:
- 100 mg/L
- Details on test conditions:
- Test vessels:The test vessels used were all plastic aquaria having the water capacity of 5liters.
- Reference substance (positive control):
- not specified
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Sublethal observations / clinical signs:
Mortality and symptoms
NominalConcentration
(mg/l)
Number of fish
Mortality (cumulated) after
3 h
6 h
24h
48h
72h
96h
V1
V2
V1
V2
V1
V2
V1
V2
V1
V2
V1
V2
0 (control)
4
4
-
-
-
-
-
-
-
-
-
-
-
-
100
4
4
-
-
-
-
-
-
-
-
-
-
-
-
Visible Symptoms:
NominalConcentration
(mg/l)
Number of fish
Symptoms after
3 h
6 h
24h
48h
72h
96h
V1
V2
V1
V2
V1
V2
V1
V2
V1
V2
V1
V2
0 (control)
4
4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
100
4
4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
Explanation of symptoms:
A:apathy
N:Narcotic-like state
C:Swimming near water surface
M: Slow movements compared to control
NS:No abnormal symptoms
S:Surfacing behaviour
L:Loss of equilibrium
SM:Shimming movement
H1:Hyperactivity
H2:Hypo activity
B:Bending behavior
F:Fin rot
IS:Invert Swimming
SB:Swimming behaviour
RF:Respiratory Function
P:Pigmentation
pH and oxygen content:
NominalConcentration
(mg/l)
Number of fish
pH readings after
24h
48h
72h
96h
V1
V2
V1
V2
V1
V2
V1
V2
0 (control)
4
4
7.26
7.27
7.41
7.46
8.11
8.12
8.17
8.19
100
4
4
7.71
7.22
7.92
7.93
8.05
8.06
8.21
8.23
NominalConcentration
(mg/l)
Number of fish
Temperature (°C) after
24h
48h
72h
96h
V1
V2
V1
V2
V1
V2
V1
V2
0 (control)
4
4
7.1
7.5
7.1
7.6
6.9
6.3
5.4
5.4
100
4
4
6.9
7.2
7.4
7.5
6.2
6.1
6.7
6.9
Test temperature
NominalConcentration
(mg/l)
Number of fish
Oxygen content (mg/L) after
24h
48h
72h
96h
V1
V2
V1
V2
V1
V2
V1
V2
0 (control)
4
4
22
22
22
22
22
22
22
22
100
4
4
22
22
22
22
22
22
22
22
- Validity criteria fulfilled:
- not specified
- Conclusions:
- In short term toxicity to fish based on nominal concentrations, experimental median lethal Concentrations [LC-50 (96 h)] for test chemical on Zebra- Fish Danio rerio was found to be >100mg/L.
- Executive summary:
Fish Acute Toxicity test according to OECD Guideline 203 was conducted for test material. The test material was soluble in water. Therefore, the test solution was prepared by dissolving 400 mg of the test substance in 1 liters of potable water (passed through reverse osmosis system) with continuous stirring. This test solution was then added to the remaining three liters of water for achieving test concentrations of 100 mg/L.The nominal concentration selected for the experiment were 100 mg/L and Zebra Fish Danio rerio were exposed to these concentration for 96 hours. The lethal concentrations LC50 was found to be >100 mg/L.
Reference
Description of key information
Short term toxicity to fish:
Fish Acute Toxicity test according to OECD Guideline 203 was conducted for test material.The test material was soluble in water. Therefore, the test solution was prepared by dissolving 400 mg of the test substance in 1 liters of potable water (passed through reverse osmosis system) with continuous stirring. This test solution was then added to the remaining three liters of water for achieving test concentrations of 100 mg/L.The nominal concentration selected for the experiment were 100 mg/L and Zebra FishDanio reriowere exposed to these concentration for 96 hours.The lethal concentrations LC50 was found to be >100 mg/L.
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 100 mg/L
Additional information
Short term toxicity to fish:
Various studies of short term toxicity to fish from experimental study report and peer reviewed journals for the target chemical were reviewed to summarize the following information:
Fish Acute Toxicity test according to OECD Guideline 203 was conducted for test material.The test substance was soluble in water. Therefore, the test solution was prepared by dissolving 400 mg of the test substance in 1 liters of potable water (passed through reverse osmosis system) with continuous stirring. This test solution was then added to the remaining three liters of water for achieving test concentrations of 100 mg/L. The nominal concentration selected for the experiment were 100 mg/L and Zebra Fish Danio rerio were exposed to these concentration for 96 hours. The lethal concentrations LC50 was found to be >100 mg/L. Experimental key study report indicate that the Fish Acute Toxicity test according to OECD Guideline 203 was conducted for test chemical .The nominal concentration selected for the experiment were 100 mg/L and Zebra Fish-Danio rerio were exposed to these concentration for 96 hours. The lethal concentrations LC50 was datarmine to be >100 mg/L.
Other supporting study from peer reviewed journal showed short term toxicity study on Fathead Minnows (Pimephales promelas) which was treated with Benzyl triethyl ammonium chloride in a measured concentration of 0, 46.5, 71.5, 110, 170 and 262 mg/l for 96 hours. 50 % mortality was observed at 161 mg/l at confidence limit of 141- 185 mg/l. Loss of schooling behavior, hyperactive and over reactive to external stimuli were observed in treated fish and were darkly colored. Equilibrium loss was not observed prior to death. Therefore, LC50 was considered to be 161 mg/l when Fathead Minnows (Pimephales promelas) treated with test material for 96 hours.
Above studies supported by another authorative database as study indicate that acute toxicity to fish test has been carried out to evaluate the ecotoxicological effects of chemical with dose concentration of 100 mg/L, 180 mg/L, 320 mg/L, 560 mg/L and 1000 mg/L. The test organism was Oryzias latipes and the test carried out in semi-static condition throughout the test temperature maintained at 24 +/- 1 degree C. The 96-h LC50 value was observed to be 694 mg/L when exposed to Oryzias latipes with test chemical.
Thus on the basis of the above experimental key study results and peer reviewed journals concluded that the target chemical can be non- hazardous to fish and therefore not consider for the further classification as per the CLP criteria.
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