Registration Dossier
Registration Dossier
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EC number: 203-643-7 | CAS number: 109-06-8
Specific investigations: other studies
Administrative data
- Endpoint:
- biochemical or cellular interactions
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Non guideline study. GLP-status unknown. Published in peer reviewed literature. Restrictions in design and reporting but adequate for assessment.
Data source
Reference
- Reference Type:
- publication
- Title:
- Pyridine and imidazole reversibly inhibit the respiratory burst in porcine and human neutrophils: Evidence for the involvement of cytochrome b558 in the reaction
- Author:
- Iizuka T., Kanegasaki S., Makino R., Tanaka T., Ishimura Y.
- Year:
- 1 985
- Bibliographic source:
- Biochemical And Biophysical Research Communications, Vol 130(2), 621-626
Materials and methods
- Principles of method if other than guideline:
- Porcine and human neutrophils were isolated from peripheral blood. Oxygen consumption was measured spectrophotometrically after addition of the test substance.
- GLP compliance:
- not specified
- Type of method:
- in vitro
- Endpoint addressed:
- other: Respiratory burst
Test material
- Reference substance name:
- 2-methylpyridine
- EC Number:
- 203-643-7
- EC Name:
- 2-methylpyridine
- Cas Number:
- 109-06-8
- Molecular formula:
- C6H7N
- IUPAC Name:
- 2-methylpyridine
- Details on test material:
- - Name of test material (as cited in study report): 2-methylpyridine
Constituent 1
Test animals
- Species:
- other: Porcine and human neutrophils
Administration / exposure
- Details on study design:
- Porcine and human neutrophils were isolated from peripheral blood. Oxygen consumption was measured. The cells in Hanks' solution were stimulated by 100 ng/mL of PMA or 2.5 mg/mL of normal-serum opsonized zymosan. Absorption spectrophotometry at 77 K was carried out with spectrophotometer equipped with a low temperature attachment.
Results and discussion
- Details on results:
- The test substance inhibited the respiratory burst in neutrophils. Inhibition was accompanied by a spectral change in reduced cytochrome B558. Both changes were reversible.
The test substance inhibited the NADPH-dependent oxygen consumption in PMA-stimulated lysed cells. Oxygen consumption in these circumstances was restored by the addition of NADPH.
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
