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EC number: 255-965-2 | CAS number: 42844-93-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: well performed guideline like study with GLP but without confirmation of negative results by furher testing
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 982
- Report date:
- 1982
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: Green, 1976
- Deviations:
- not specified
- GLP compliance:
- yes
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- [1,3-dihydro-5,6-bis[[(2-hydroxy-1-naphthyl)methylene]amino]-2H-benzimidazol-2-onato(2-)-N5,N6,O5,O6]nickel
- EC Number:
- 255-965-2
- EC Name:
- [1,3-dihydro-5,6-bis[[(2-hydroxy-1-naphthyl)methylene]amino]-2H-benzimidazol-2-onato(2-)-N5,N6,O5,O6]nickel
- Cas Number:
- 42844-93-9
- Molecular formula:
- C29H18N4NiO3
- IUPAC Name:
- Nickel, [1,3-dihydro-5,6-bis[[[2-(hydroxy-kO)-1-naphthalenyl]methylene]amino-kN]-2H-benzimidazol-2-onato(2-)]-, (SP-4-2)-
- Test material form:
- solid: nanoform
Constituent 1
Method
Species / strain
- Species / strain / cell type:
- E. coli WP2 uvr A
- Metabolic activation:
- with and without
- Metabolic activation system:
- AROCLOR 1254 induced rat liver S-9 mix
- Test concentrations with justification for top dose:
- 1.58 / 5 / 15.8 / 50 / 158 / 500 / 1580 AND 5000 µg/plate
- Vehicle / solvent:
- DIMETHYLSULFOXIDE
Controls
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- other: METHYL METHANE SULFONATE, N-ETHYL-N-NITRO-N-NITROSOGUANIDINE, 2-AMINOANTHRACENE
- Details on test system and experimental conditions:
- METHOD OF APPLICATION: plate incorporation
NUMBER OF REPLICATIONS: TRIPLICATE PLATES, without confirmation of negative results by further testing - Evaluation criteria:
- SPECIAL ATTENTION WAS GIVEN TO A REPRODUCIBLE DOSE-EFFECT RELATIONSHIP.
A MUTAGENIC ACTIVITY IS ASSUMED IF AT LEAST A TWO FOLD INCREASE OF THE NUMBER OF INDUCED REVERTANTS IS OBTAINED IN COMPARISON WITH THE SPONTANEOUS REVERTANTS OF THE CORRESPONDING CONTROL.
Results and discussion
Test results
- Key result
- Species / strain:
- E. coli WP2 uvr A
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not examined
- Positive controls validity:
- valid
Applicant's summary and conclusion
- Conclusions:
- IN CONCLUSION IT CAN BE STATED THAT DURING THIS IN VITRO ESCHERICHIA COLI REVERSE MUTATION ASSAY NO GENE MUTAGENIC ACTIVITY COULD BE DEMONSTRATED UNDER THE EXPERIMENTAL CONDITIONS REPORTED.
- Executive summary:
THE TEST ITEM WAS TESTED FOR DETECTING ITS POTENTIAL GENE MUTAGENIC ACTIYITY ACCORDING TO THE PLATE INCORPORATION METHOD OF GREEN ET AL, USING THE ESCHERICHIA COLI STRAIN WP2UVRA.
THE TEST WAS PERFORMED WITH AND WITHOUT LIVER MICROSOMAL ACTIYATION. THE TEST MATERIAL WAS TESTED AT EIGHT CONCENTRATIONS: 1.58 / 5 / 15.8 / 50 / 158 / 500 / 1580 AND 5000 MICROGRAMS (MCG) PER PLATE. EACH COMPOUND CONCENTRATION INCLUDING CONTROLS WAS TESTED IN TRIPLICATE.
A VERY SLIGHT PRECIPITATION OF THE TEST MATERIAL WAS OBSERYED AFTER POURING THE CONTENT OF THE TEST TUBE OVER THE SURFACE OF THE SELECTIYE AGAR PLATE AT THE CONCENTRATIONS OF 1580 AND 5000 MCG PER PLATE. THE REVERTANTS WERE EASILY RECOGNIZABLE AND THE PLATES COULD BE EYALUATED.
UP TO THE HIGHEST TESTED DOSE, WITH AND WITHOUT LIVER MICROSOMAL ACTIYATION, NO RELEVANT INCREASE OF THE REVERTANT COLONY NUMBERS WAS OBTAINED IN COMPARISON WITH THE CORRESPONDING CONTROLS.
NO TOXIC EFFECTS OF THE TEST MATERIAL WERE OBSERVED.
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