4-isopropylbenzonitrile

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2015

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP compliant study conducted according to OECD Guidelines, with no or minor deviations.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2015

Report date:

2015

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

4-isopropylbenzonitrile

EC Number:

237-492-3

EC Name:

4-isopropylbenzonitrile

Cas Number:

13816-33-6

Molecular formula:

C10H11N

IUPAC Name:

4-(propan-2-yl)benzonitrile

Test material form:

other: liquid

Details on test material:

Batch SC00008199
Purity 99.6%
Expiry date 2015-02-26

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

yes

Details on test solutions:

6 test item concentrations in a geometric series with a separation factor of 2.2, prepared by diluting the stock solution of 10.0 mg/L with dilution water, were tested as follows:0.194 - 0.427 - 0.939 - 2.07 - 4.55 - 10.0 mg/L.

Test organisms

Test organisms (species):

Daphnia magna

Study design

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Nominal and measured concentrations:

Nominal Concentrations: 0.194 - 0.427 - 0.939 - 2.07 - 4.55 - 10.0 mg/L.

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

9.79 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: (Cl: 4.87 – 10.0)

Effect concentrations 2

Duration:

24 h

Dose descriptor:

EC10

Effect conc.:

9.09 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: (Cl: 4.60 – 10.0)

Effect concentrations 3

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

6.13 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: (Cl: 5.26 – 7.00)

Effect concentrations 4

Duration:

48 h

Dose descriptor:

EC10

Effect conc.:

2.96 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: (Cl: 2.40 – 3.66)

Effect concentrations 5

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

2.07 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

The measured concentrations of the test item at the start of the exposure intervals (0 and 24 h) were in the range of 98 to 100 % of the nominal values. At the end of the exposure intervals (24 and 48 h), the measured concentrations of the test item were in the range of 81 to 90 % of the nominal values.
The measured test item concentrations were all within ± 20 % of the nominal concentrations. This indicates that the test item concentrations were successfully maintained for the duration of the test. Therefore, the effect levels given are based on the nominal concentrations of the test item Cumin
Nitrile.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The 48 h-EC50 for Daphnia magna was 6.13 mg/L (95 % confidence limits: 5.26 – 7.00 mg/L).