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Diss Factsheets
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EC number: 414-310-2 | CAS number: 191358-81-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2007-12-31 to 2008-02-09
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP guideline Study (OECD 203) including analytical monitoring.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 008
- Report date:
- 2008
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.1 (Acute Toxicity for Fish)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- other: JMAFF Test Guidelines, 2-7-1-1, Acute fish toxicity, 2005
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: limit concentration and controls
- Sampling method: The water temperature, pH and dissolved oxygen concentrations were measured at the start of the test and once every day during the test at the single test concentration and in the control. At the same dates the appearance of the test medium was recorded. For the analysis of the actual test item concentrations the following samples were taken:
Just before test start:
- duplicate samples from the test medium
- one of the duplicate samples from the control
after 48 and 96 hours:
- duplicate samples from the test medium
- duplicate samples from the control at 48 hours (one of the duplicate control samples at 96 hours)
- Sample storage conditions before analysis: All samples were taken from the approximate center of the aquaria without mixing the test media, and were deep-frozen (at about -20 °C) immediately after sampling. Based on pre-experiments for investigation of the storage stability (without GLP) the test item is sufficiently stable in the test water under the storage conditions.
Test solutions
- Vehicle:
- no
- Details on test solutions:
- For the preparation of the test medium, a dispersion of the test item with the loading rate of 100 mg/L was prepared. The dispersion was stirred for 3 hours to dissolve a maximum amount of the different compounds of the test item in the dispersion. Then, the dispersion was filtered through a membrane filter (0.45 μm pore size) and the undiluted filtrate was tested as WAF.
Test organisms
- Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- TEST ORGANISM
- the study was performed with zebra fish (Brachydanio rerio).
- the test fish were raised in the RCC laboratories without any medication.
- From the acclimated test fish batch, 10 fish were measured at the start of the test.
- The mean body length of the fish was 2.9 ± 0.14 cm (Mean ± SD), the mean body wet weight was 0.23 ± 0.06 g (Mean ± SD).
ACCLIMATION
- Prior to test start, they were acclimated for one week to the test water and temperature.
- During holding and acclimatization until one day before the start of the test the fish were fed with a commercial fish diet .
- During the last two weeks prior to the test no fish died in the test fish batch and all fish were healthy.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 96 h
Test conditions
- Hardness:
- 125 mg/L as CaCO3
- Test temperature:
- 21 +/- 0 °C
- pH:
- 7.2 - 7.4
- Dissolved oxygen:
- 8.0 - 8.4 mg/L
- Nominal and measured concentrations:
- Since a WAF of the test item with sparingly soluble components was tested, all reported biological results are based on the loading rate of 100 mg/L. The analytically measured test item concentration in the test medium was in the range of 0.97 to 1.3 μg/L during the test period of 96 hours.
- Details on test conditions:
- - One glass test vessel with 5 liters of test medium was used for each treatment (the single test concentration and the control).
- At the start of the test 7 fish were introduced into each test vessel in a random order.
- The loading rate was less than 1 g fish wet weight per liter test medium.
- The test medium and the control were slightly aerated during the test period.
- The fish were not fed during the test.
A static test without test medium renewal was performed under the following conditions:
- Test duration: 96 hours
- Water temperature: 21 °C during the test period.
- Light conditions: A 16-hour light to 8-hour dark photoperiod, with a 30-minute transition period (light intensity during the light period was approximately within the range of 100 to 560 Lux).
- Reconstituted test water was used in the study. - Reference substance (positive control):
- no
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: WAF (Water Accomodated Fraction); no acute toxic effects within the range of solubility.
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: LC50 was estimated; no acute toxic effects within the range of solubility.
- Details on results:
- A limit test was performed in accordance with the test guidelines to demonstrate that water accommodated fractions (WAFs) of the test item have no toxic effect on the test fish up to the loading rate of 100 mg/L. Thus, the only treatment tested was a WAF with the loading rate of nominal 100 mg/L in parallel to a control.
- Results with reference substance (positive control):
- NA
- Reported statistics and error estimates:
- NA
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- With high probability not acutely harmful to fish. No acute toxic effects within the range of solubility.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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