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EC number: 440-050-4 | CAS number: 243857-97-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Hydrolysis
Administrative data
Link to relevant study record(s)
- Endpoint:
- hydrolysis
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- From Jun. 11, 1999 to Dec. 14, 1999
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.7 (Degradation: Abiotic Degradation: Hydrolysis as a Function of pH)
- Deviations:
- no
- Principles of method if other than guideline:
- Not applicable
- GLP compliance:
- yes
- Radiolabelling:
- no
- Analytical monitoring:
- yes
- Details on sampling:
- - Sampling method:
Preparation of Test Samples: Solutions of the test substance were prepared by weighing between 10 and 15 mg of the test substance in a 50 mL volumetric flask and dissolving in the relevant buffer. The resulting solution was subjected immediately to the hydrolysis procedure.
Preparation of the Standards: The calculation standards were freshly prepared by weighing between 7 and 16 mg of the standard test substance in a 50 mL volumetric flask and dissolving in the buffer pH 4 7 or 9.
- Sample storage conditions before analysis: Not stored - Buffers:
- - pH: 4
- Composition of buffer: 21.008 g citric acid monohydrate were dissolved in 200 mL sodium hydroxide solution (c=1 mole/L). This solution was filled up to a volume of 1000 mL with distilled water. 44 mL of hydrochloric acid (c=1 mole/L) were added to 560 mL of this solution and filled up to a volume of 1000 mL with distilled water.
- pH: 7
- Composition of buffer: 13.61 g potassium dihydrogen phosphate were dissolved in 1000 mL distilled water. 29.63 mL of sodium hydroxide solution (c=1 mole/L) were added to 500 mL of this solution and filled up to a volume of 1000 mL with distilled water.
- pH: 9
- Composition of buffer: 7.46 g potassium chloride and 6.184 g boric acid were dissolved in 1000 mL water. 500 mL of this solution were added to 21.3 mL sodium hydroxide (c=1 mole/L) and filled up to a volume of 1000 mL with water.
For performing the hydrolysis the solution was refluxed and the pH value was adjusted to respective pH (4, 7 and 9) for the each hydrolysis temperature. - Details on test conditions:
- TEST SYSTEM
- Type, material and volume of test flasks, other equipment used: Flask
Test Procedure
The flask containing the hydrolysis mixture was placed in a thermostatically controlled water bath at the selected temperature for the specified time. Thereafter it was cooled immediately. The pH was controlled.
-Analysis: HPLC analysis of the unhydrolysed test substance (main compound)
OTHER TEST CONDITIONS
- Adjustment of pH: Yes - Duration:
- 5 d
- pH:
- 4
- Temp.:
- 50 °C
- Initial conc. measured:
- 257.2 - 266.24 mg/L
- Duration:
- 5 d
- pH:
- 7
- Temp.:
- 50 °C
- Initial conc. measured:
- 254.88 - 272.92
- Duration:
- 5 d
- pH:
- 9
- Temp.:
- 50 °C
- Initial conc. measured:
- 263.44 - 265.32
- Number of replicates:
- Two
- Positive controls:
- no
- Negative controls:
- no
- Preliminary study:
- The decomposition at pH 9 is higher than 50 % after 2.4 h at 50 °C. Thus the test substance may be considered hydrolytically instable at pH 9. According to the guideline a half-life period of less than one day under environmental conditions (25 °C) may be estimated. The pretests at pH 4 and at pH 7 indicate that an examination of the hydrolysis kinetics is essential.
- Transformation products:
- no
- Details on hydrolysis and appearance of transformation product(s):
- Not applicable
- pH:
- 4
- Temp.:
- 25 °C
- DT50:
- 7.95 d
- Type:
- other: Extrapolated value
- Remarks on result:
- other: Extrapolation according to the Arrhenius equation
- pH:
- 7
- Temp.:
- 25 °C
- DT50:
- 12.6 d
- Type:
- other: Extrapolated value
- Remarks on result:
- other: Extrapolation according to the Arrhenius equation
- pH:
- 4
- Temp.:
- 50 °C
- Hydrolysis rate constant:
- 0.03 h-1
- DT50:
- 22.76 h
- Type:
- (pseudo-)first order (= half-life)
- Remarks on result:
- other: correlation: r = 0.9978
- pH:
- 4
- Temp.:
- 60 °C
- Hydrolysis rate constant:
- 0.068 h-1
- DT50:
- 10.27 h
- Type:
- (pseudo-)first order (= half-life)
- Remarks on result:
- other: correlation: r =0.9998
- pH:
- 4
- Temp.:
- 70 °C
- Hydrolysis rate constant:
- 0.134 h-1
- DT50:
- 5.16 h
- Type:
- (pseudo-)first order (= half-life)
- Remarks on result:
- other: correlation: r = 0.9995
- pH:
- 7
- Temp.:
- 50 °C
- Hydrolysis rate constant:
- 0.061 h-1
- DT50:
- 11.31 h
- Type:
- (pseudo-)first order (= half-life)
- Remarks on result:
- other: correlation: r = 0.9970
- pH:
- 7
- Temp.:
- 60 °C
- Hydrolysis rate constant:
- 0.198 h-1
- DT50:
- 3.5 h
- Type:
- (pseudo-)first order (= half-life)
- Remarks on result:
- other: correlation: r = 0.9998
- pH:
- 7
- Temp.:
- 70 °C
- Hydrolysis rate constant:
- 0.603 h-1
- DT50:
- 1.15 h
- Type:
- (pseudo-)first order (= half-life)
- Remarks on result:
- other: correlation: r = 1.0000
- Details on results:
- TEST CONDITIONS
- pH, sterility, temperature, and other experimental conditions maintained throughout the study: Yes - Validity criteria fulfilled:
- yes
- Conclusions:
- Under the test conditions, the extrapolated half life values of the test substance at pH 4 and 7 were found to be 7.95 and 12.6 d respectively at 25 °C. At pH 9 the decomposition is higher than 50 % after 2.4 h at 50 °C. Thus the test substance may be considered hydrolytically instable at pH 9.
- Executive summary:
A study was performed to determine the abiotic degradation of the test substance, i.e. the determination of the hydrolysis rate as a function of the pH-value according to EU Method C.7. in compliance with GLP.
Solutions of the test substance were prepared by weighing between 10 and 15 mg of the test substance in a 50 mL volumetric flask and dissolving in the relevant buffer. The resulting solution was subjected immediately to the hydrolysis procedure.
The flask containing the hydrolysis mixture was placed in a thermostatically controlled water bath at the selected temperature for the specified time. Thereafter it was cooled immediately. The pH was controlled. The HPLC analysis of the unhydrolysed test substance (main compound) was performed without any delay
At pH 4 the half-life period was determined by extrapolating the results of the experiments at 50, 60 and 70 °C to a temperature of 25 °C. It was found to be 7.95 d.
At pH 7 the half-life period was determined by extrapolating the results of the experiments at 50, 60 and 70 °C to a temperature of 25 °C. It was found to be 12.6 d.
At pH 9 the decomposition is higher than 50 % after 2.4 h at 50 °C. Thus the test substance may be considered hydrolytically instable at pH 9.
Under the test conditions, the extrapolated half life values of the test substance at pH 4 and 7 were found to be 7.95 and 12.6 d respectively at 25 °C.
Reference
Table 1. Summary of the Hydrolysis Results :
Summary and extrapolation of the results at pH 4 and 7
pH |
Temperature (°C) |
t1/2(by regression) ( h) |
Experiment |
4 |
50 |
22.76 |
Test 1 |
4 |
60 |
10.27 |
Test 2 |
4 |
70 |
5.16 |
Test 3 |
|
25 |
190.83 h=(7.95 d) |
Extrapolation |
7 |
50 |
11.31 |
Test 1 |
7 |
60 |
3.50 |
Test 2 |
7 |
70 |
1.15 |
Test 3 |
|
25 |
303.05 h=(12.6 d) |
Extrapolation |
Description of key information
The extrapolated half life values of test substance at pH 4 and 7 were found to be 7.95 and 12.6 d respectively at 25 °C. At pH 9 the decomposition is higher than 50 % after 2.4 h at 50 °C. Thus the test substance may be considered hydrolytically instable at pH 9
Key value for chemical safety assessment
- Half-life for hydrolysis:
- 7.95 d
- at the temperature of:
- 25 °C
Additional information
A study was performed to determine the abiotic degradation of the test substance i.e. the determination of the hydrolysis rate as a function of the pH-value according to EU Method C.7.
Solutions of the test substance were prepared by weighing between 10 and 15 mg of the test substance in a 50 mL volumetric flask and dissolving in the relevant buffer. The resulting solution was subjected immediately to the hydrolysis procedure.
The flask containing the hydrolysis mixture was placed in a thermostatically controlled water bath at the selected temperature for the specified time. Thereafter it was cooled immediately. The pH was controlled. The HPLC analysis of the unhydrolysed test substance (main compound) was performed without any delay
At pH 4 the half-life period was determined by extrapolating the results of the experiments at 50, 60 and 70 °C to a temperature of 25 °C. It was found to be 7.95 d.
At pH 7 the half-life period was determined by extrapolating the results of the experiments at 50, 60 and 70 °C to a temperature of 25 °C. It was found to be 12.6 d.
At pH 9 the decomposition is higher than 50 % after 2.4 h at 50 °C. Thus the test substance may be considered hydrolytically instable at pH 9.
The extrapolated half life values of test substance at pH 4 and 7 were found to be 7.95 and 12.6 d respectively at 25 °C. At pH 9 the decomposition is higher than 50 % after 2.4 h at 50 °C. Thus the test substance may be considered hydrolytically instable at pH 9 (Muhlberger B, 1999).
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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