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EC number: 241-922-5 | CAS number: 18015-76-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biological effects monitoring
Administrative data
- Endpoint:
- biological effects monitoring
- Type of information:
- migrated information: read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- weight of evidence
- Reliability:
- 4 (not assignable)
- Rationale for reliability incl. deficiencies:
- other: see 'Remark'
- Remarks:
- Data from literature; despite the global experiment is well documented and scientifically acceptable, details about the tested substance composition are missing. Read across from a similar substance which has the same main component and with a different counter ion that does not influence the characteristics related to the specific end-point.
Data source
Reference
- Reference Type:
- publication
- Title:
- Effetto tossici del verde malachite su alcuni parametri biochimici di trota iridea (Oncorhynchus mykiss, Walbaum).
- Author:
- Lanari D., Lang G., Ballestrazzi R.
- Year:
- 1 996
- Bibliographic source:
- Rivista Italiana di Acquacultura, 31(2): 59-74.
Materials and methods
- Principles of method if other than guideline:
- The enzyme activities were determined in accordance Tonhazy et al.(1950), Karmen (1955), Reitman and Frankel (1957); Amador et al.(1966).
- GLP compliance:
- not specified
- Type of study / information:
- Toxic effects of Malachite Green on some biochemical parametres of Rainbow trout.
Test material
- Reference substance name:
- Malachite Green
- IUPAC Name:
- Malachite Green
Constituent 1
Results and discussion
Any other information on results incl. tables
The activity of GOT was not influenced by malachite green, while we observed a significant increase in the activity of GPT. The changes in the activity of GPT proportional to the concentration of the product (they were influenced by exposure time). After 48 hours of exposure the enzyme activity began to decrease, returning to normal after 72 hours. After treatment with MG were observed modifications also in the activity of LDH's significant after 48, 72 and 96 hours, but not depended on the concentration of the product. The highest value was observed after 72 hours exposure to 0.4 ppm of MG.. No effect of MG you could see the activity of AChE. The only significant change was the increase after 48 hours of exposure, concentration of 0.05 ppm. Blood glucose increased during treatment with malachite green, showing a strong effect of general stress. After 48 hours of exposure, this variable is returned to normal values. The activity of hepatic microsomal dell'UDP-GT showed no significant change during treatment, while the VM inhibits the activity of GST after 48 hours. Following the values returned to normal.
Activity of LDH
Hours | 0.05 ppm | 0.20 ppm | 0.40 ppm |
24 | 72.98 ± 10-64 | 88.55 ± 57.47 | 123.63 ± 40.76 |
48 | 140.93 ± 48.90 | 168.75 ± 32.03 | 146.01 ± 58.89 |
72 | 112.99 ± 40.27 | 106.74 ± 19.22 | 244.48 ± 55.33 |
96 | 191.23 ± 54.27 | 110.39 ± 38.12 | 173.16 ± 66.27 |
Control: 1102.75 ± 439.06 |
Activity of AChE
Hours | 0.05 ppm | 0.20 ppm | 0.40 ppm |
24 | 117.27 ± 15.62 | 87.59 ± 20.81 | 96.93 ± 20.21 |
48 | 156.26 ± 39.00 | 112.05 ± 22.54 | 118.68 ± 19.18 |
72 | 96.79 ± 24.28 | 101.98 ± 39.51 | 128.15 ± 22.99 |
96 | 117.70 ± 43.80 | 127.38 ± 50.37 | 93.28 ± 26.105 |
Control: 99.15 ± 38.82 |
Rate of glucose
Hours | 0.05 ppm | 0.20 ppm | 0.40 ppm |
24 | 70.90 ± 12.07 | 109.82 ± 8.84 | 145.00 ± 19.04 |
48 | 171.58 ± 28.75 | 172.76 ± 17.49 | 171.11 ± 24.32 |
72 | 168.76 ± 15.19 | 80.07 ± 14.32 | 104.51 ± 23.70 |
96 | 102.91 ± 13.92 | 110.77 ± 21.23 | 106.63 ± 23.24 |
Control: 85.43 ± 24.16 |
Applicant's summary and conclusion
- Conclusions:
- The results show that Malachite Green does not interfere on the nervous system, but causes damage to the liver and inhibits the process of conjugation.
- Executive summary:
One hundred rainbow trout (100 ± 20 g) were randomly divided into four plastic tanks (300 l) and exposed to four concentrations (0.00, 0.05, 0.20, 0.40 ppm) of malachite green for 24, 48, 72 and 96 hours. At the end of each period of exposure, 5 fish per tank were subjected to blood sampling to determine blood glucose and the activity of plasma enzymes (GOT, GPT, LDH and AChE),and sampling to determine the activity of liver hepatic microsomal enzymes (UDP-GT and GST). MG has little effect on AChE and GOT, and LDH, GPT and glucose increased after 48 hours of exposure. The activity of UDP-GT was not affected by the MG, while GST was inhibited only after 48 hours of exposure.
Conclusion
The results show that Malachite Green does not interfere on the nervous system, but causes damage to the liver and inhibits the process of conjugation.
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