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Diss Factsheets
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EC number: 211-219-8 | CAS number: 634-93-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://echa.europa.eu/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_toxicological-information.png)
Genetic toxicity: in vivo
Administrative data
- Endpoint:
- in vivo mammalian germ cell study: gene mutation
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- data from handbook or collection of data
- Justification for type of information:
- Data is from peer reviewed publication
Data source
Reference
- Reference Type:
- publication
- Title:
- Gene mutation toxcity in vivo for the test chemical
- Author:
- Kugler-Steigmeier et al
- Year:
- 1 989
- Bibliographic source:
- Mutation Research
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: Refer below principle
- Principles of method if other than guideline:
- The test chemical was investigated for genotoxicity in Drosophila.
- GLP compliance:
- not specified
- Type of assay:
- somatic mutation and recombination test in Drosophila
Test material
- Reference substance name:
- 2,4,6-trichloroaniline
- EC Number:
- 211-219-8
- EC Name:
- 2,4,6-trichloroaniline
- Cas Number:
- 634-93-5
- Molecular formula:
- C6H4Cl3N
- IUPAC Name:
- 2,4,6-trichloroaniline
- Details on test material:
- - Name of test material (as cited in study report): 2,4,6 trichloroaniline
- Molecular formula (if other than submission substance): C6-H4-Cl3-N
- Molecular weight (if other than submission substance): 196.464
- Substance type: organic
- Physical state: solid
- Purity: > 99%
Constituent 1
Test animals
- Species:
- Drosophila melanogaster
- Strain:
- not specified
- Sex:
- not specified
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS:
- Age at study initiation: 72 hr old larvae
- Diet (e.g. ad libitum): Drosophila Instant Medium
Administration / exposure
- Route of administration:
- oral: feed
- Vehicle:
- - Vehicle(s)/solvent(s) used: absolute ethanol and Tween 80
- Concentration of test material in vehicle: 5mM (9823 mg/L)
- Amount of vehicle (if gavage or dermal): No data - Details on exposure:
- Details on exposure
For oral route
PREPARATION OF DOSING SOLUTIONS: Test compound were dissolved in an aqueous solution containing 3% absolute ethanol and 1% Tween 80 and used to prepare Drosophila Instant Medium (Formula 4- 24, Carolina Biological Supply Company, Burlington, NC, U.S.A.). - Duration of treatment / exposure:
- 48 hrs
- Frequency of treatment:
- No data available
- Post exposure period:
- No data available
Doses / concentrations
- Remarks:
- Doses / Concentrations:
5mM (9823 mg/L)
Basis:
nominal in diet
- No. of animals per sex per dose:
- No data
- Control animals:
- yes, concurrent vehicle
- Positive control(s):
- No data
Examinations
- Tissues and cell types examined:
- Multiple wing hair and flare
- Details of tissue and slide preparation:
- No data
- Evaluation criteria:
- Induced spots on wings were examined acc. to Graf et al.
- Statistics:
- Statistical analysis of the data was carried out as described by Frei and Würgler (1988).
Results and discussion
Test results
- Sex:
- not specified
- Genotoxicity:
- positive
- Toxicity:
- not specified
- Vehicle controls validity:
- not specified
- Negative controls validity:
- not specified
- Positive controls validity:
- not specified
- Additional information on results:
- No data
Any other information on results incl. tables
Compound and concentration |
Number of wings |
Spots per wing and statistical diagnoses |
|||
Small single spots m= 2.0 |
Large single spots m= 5.0 |
Twin spots m= 5.0 |
Total spots m= 2.0 |
||
2,4,6-TCA 5 mM |
80 |
0.45 + |
0.08 - |
0.01 i |
0.54 + |
Note: += positive; -= negative; i= inconclusive
Applicant's summary and conclusion
- Conclusions:
- The test chemical is considered to be positive for inducing somatic and recombinant mutation using Drosophila melanogaster.
- Executive summary:
Gene mutation in vivo was performed for the test chemical using Drosophila melanogaster. 72-h-old larvae of Drosophila melanogaster, trans-heterozygous for the mutations multiple wing hair (mwh, 3-0.0) and flare (fir, 3-38.8), were fed the test compound (5mM (9823 mg/L)) for 48 h. Test compound was dissolved in an aqueous solution containing 3% absolute ethanol and 1% Tween 80 and used to prepare Drosophila Instant Medium (Formula 4- 24, Carolina Biological Supply Company, Burlington, NC, U.S.A.) at dose of 5mM. In order to determine the spontaneous mutation frequency, additional larvae were simultaneously exposed to the solvent only. The larvae were treated, and the wings prepared and scored for induced spots. Statistical analysis of the data was carried out as described by Frei and Würgler (1988). The test chemical is considered to be positive for inducing somatic and recombinant mutation using Drosophila melanogaster.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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