2,4,6-trichloroaniline

Genetic toxicity: in vivo

Administrative data

Endpoint:

in vivo mammalian germ cell study: gene mutation

Remarks:

Type of genotoxicity: gene mutation

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

data from handbook or collection of data

Justification for type of information:

Data is from peer reviewed publication

Data source

Reference

Reference Type:

publication

Title:

Gene mutation toxcity in vivo for the test chemical

Author:

Kugler-Steigmeier et al

Year:

1989

Bibliographic source:

Mutation Research

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: Refer below principle

Principles of method if other than guideline:

The test chemical was investigated for genotoxicity in Drosophila.

GLP compliance:

not specified

Type of assay:

somatic mutation and recombination test in Drosophila

Test material

Test material information

Constituent 1

Reference substance name:

2,4,6-trichloroaniline

EC Number:

211-219-8

EC Name:

2,4,6-trichloroaniline

Cas Number:

634-93-5

Molecular formula:

C6H4Cl3N

IUPAC Name:

2,4,6-trichloroaniline

Details on test material:

- Name of test material (as cited in study report): 2,4,6 trichloroaniline
- Molecular formula (if other than submission substance): C6-H4-Cl3-N
- Molecular weight (if other than submission substance): 196.464
- Substance type: organic
- Physical state: solid
- Purity: > 99%

Test animals

Species:

Drosophila melanogaster

Strain:

not specified

Sex:

not specified

Details on test animals or test system and environmental conditions:

TEST ANIMALS:
- Age at study initiation: 72 hr old larvae
- Diet (e.g. ad libitum): Drosophila Instant Medium

Administration / exposure

Route of administration:

oral: feed

Vehicle:

- Vehicle(s)/solvent(s) used: absolute ethanol and Tween 80
- Concentration of test material in vehicle: 5mM (9823 mg/L)
- Amount of vehicle (if gavage or dermal): No data

Details on exposure:

Details on exposure
For oral route
PREPARATION OF DOSING SOLUTIONS: Test compound were dissolved in an aqueous solution containing 3% absolute ethanol and 1% Tween 80 and used to prepare Drosophila Instant Medium (Formula 4- 24, Carolina Biological Supply Company, Burlington, NC, U.S.A.).

Duration of treatment / exposure:

48 hrs

Frequency of treatment:

No data available

Post exposure period:

No data available

Doses / concentrations

Remarks:

Doses / Concentrations:
5mM (9823 mg/L)
Basis:
nominal in diet

No. of animals per sex per dose:

No data

Control animals:

yes, concurrent vehicle

Positive control(s):

No data

Examinations

Tissues and cell types examined:

Multiple wing hair and flare

Details of tissue and slide preparation:

No data

Evaluation criteria:

Induced spots on wings were examined acc. to Graf et al.

Statistics:

Statistical analysis of the data was carried out as described by Frei and Würgler (1988).

Results and discussion

Test results

Sex:

not specified

Genotoxicity:

positive

Toxicity:

not specified

Vehicle controls validity:

not specified

Negative controls validity:

not specified

Positive controls validity:

not specified

Additional information on results:

No data

Any other information on results incl. tables

Compound and concentration	Number of wings	Spots per wing and statistical diagnoses
		Small single spots m= 2.0	Large single spots m= 5.0	Twin spots m= 5.0	Total spots m= 2.0
2,4,6-TCA 5 mM	80	0.45 +	0.08 -	0.01 i	0.54 +

Note: += positive; -= negative; i= inconclusive

Applicant's summary and conclusion

Conclusions:

The test chemical is considered to be positive for inducing somatic and recombinant mutation using Drosophila melanogaster.

Executive summary:

Gene mutation in vivo was performed for the test chemical using Drosophila melanogaster. 72-h-old larvae of Drosophila melanogaster, trans-heterozygous for the mutations multiple wing hair (mwh, 3-0.0) and flare (fir, 3-38.8), were fed the test compound (5mM (9823 mg/L)) for 48 h. Test compound was dissolved in an aqueous solution containing 3% absolute ethanol and 1% Tween 80 and used to prepare Drosophila Instant Medium (Formula 4- 24, Carolina Biological Supply Company, Burlington, NC, U.S.A.) at dose of 5mM. In order to determine the spontaneous mutation frequency, additional larvae were simultaneously exposed to the solvent only. The larvae were treated, and the wings prepared and scored for induced spots. Statistical analysis of the data was carried out as described by Frei and Würgler (1988). The test chemical is considered to be positive for inducing somatic and recombinant mutation using Drosophila melanogaster.