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EC number: 603-033-4 | CAS number: 1251-28-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- Oct 1996
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: According to OECD guideline under GLP
Cross-referenceopen allclose all
- Reason / purpose for cross-reference:
- reference to same study
- Reason / purpose for cross-reference:
- reference to other study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Report date:
- 1997
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Deviations:
- no
- Principles of method if other than guideline:
- Not relevant
- GLP compliance:
- yes
Test material
- Reference substance name:
- 21-Acetoxy-6 alpha-fluoro-16 alpha-methyl-4-pregnene-3,20-dione
- EC Number:
- 603-033-4
- Cas Number:
- 1251-28-1
- Molecular formula:
- C24 H33 F O4
- IUPAC Name:
- 21-Acetoxy-6 alpha-fluoro-16 alpha-methyl-4-pregnene-3,20-dione
- Details on test material:
- - Name of test material (as cited in study report): Alpha-FM-DOCA
- Analytical Purity: 71%
- Lot/batch No.: 21602001
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
Test solutions
- Vehicle:
- no
Test organisms
- Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 1.1 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: 0.3-1.8 mg/L
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 0.3 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
Any other information on results incl. tables
Table 1: Inhibition of the growth of Scenedesmus subspicatus after 72 hours exposure to ZK 4841
Dilution | Concentration | Inhibition in percent | |
of ZK 46865 | (mg/I) | Biomass (integral) | Growth rate |
Control | 0 | 0 | 0 |
1 :100 | 0,05 | 3,8 | 1,4 |
1: 50 | 0,11 | -13,9 | -5,4 |
1: 25 | 0,22 | 38,4 | 10,6 |
1: 13 | 0,43 | 68,8 | 32,5 |
1: 6 | 0,87 | 82,3 | 49 |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- ZK 4841 had an inhibitory, concentration-related effect on the growth of Scenedesmus subspicatus. The EbC50 (biomass) and the ErC50 (growth
rate) were estimated on the basis of the calculated concentrations of ZK 4841 using the organic carbon concentration in the stock
solution. The EbC50 was 0.2 mg/L (no confidence interval could be given), the ErC50 was 1.1 mg/L (95% confidence interval: 0.3-1.8). - Executive summary:
The purpose of this study was to determine the effects of the test compound Alpha-FM-DOCA (ZK 4841) on the growth of the green algae Scenedesmus subspicatus.
The test substance was incubated in an aqueous solution including nutrients with an algae population of Scenedesmus subspicatus for a test duration of approximately 72 hours. The nutrient solution was made up of mainly nitrate, phosphates and some trace elements. For the preparation of the test solution, a suspension of 100 mg/I was stirred for 24 hours. This suspension was filtered through a glass fibre filter. The saturated solution obtained by this procedure was used for the highest test concentration. It was further diluted
1:6.3, 1:12.5, 1:25, 1:50 and 1:100. The algae were exposed to each concentration in triplicate. Six vessels were prepared for the control.
As a parameter for the growth of the algae population, the cell concentrations of the test and control solutions were counted with an electronic particle counter ("Coulter Counter"). The increase of biomass and the growth rate were calculated on the basis of the cell counts. The calculated biomass and growth rate of each concentration were compared to those of the controls and the inhibition was calculated. The following table gives the inhibition in percent on the basis of the biomass (integral) and the growth rate.
The measurements showed that there is a decrease in cell concentration over the test period. The resulting growth inhibition was concentration-related.
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