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Diss Factsheets
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EC number: 202-905-8 | CAS number: 100-97-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- other: studyreport
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 980
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: National Environmental Research Center, 1971: Algal assay procedure: bottle test. US EPA Corvallis, Oregon
- GLP compliance:
- no
Test material
- Reference substance name:
- Methenamine
- EC Number:
- 202-905-8
- EC Name:
- Methenamine
- Cas Number:
- 100-97-0
- Molecular formula:
- C6H12N4
- IUPAC Name:
- 1,3,5,7-tetraazatricyclo[3.3.1.1³,⁷]decane
- Details on test material:
- not indicated
Constituent 1
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material (migrated information):
not indicated
Sampling and analysis
- Analytical monitoring:
- no
- Details on sampling:
- no sampling
Test solutions
- Vehicle:
- no
Test organisms
- Test organisms (species):
- Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- Growth medium used: Bold's mineral alsts media, concentration of algae: 10E03 cells/ml
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 14 d
- Post exposure observation period:
- no post exposure period
Test conditions
- Hardness:
- not indicated
- Test temperature:
- not indicated
- pH:
- 7,5
- Dissolved oxygen:
- not indicated
- Salinity:
- freshwater used
- Nominal and measured concentrations:
- nominal: 0, 1000, 2500 and 5000 mg/L
- Details on test conditions:
- Selenastrum capricornutum was exposed in a 14 d growth inhibition test following U.S. EPA (AAP: BT) guidelines. Bold's mineral salts medium was slightly modified by adding tris buffer and NaHCO3 and an illumination regime of 12 h light/12 h dark was applied. The pH of the medium was 7.5. The maximum specific growth rates for 1, 2.5 and 5 g methenamine/l (dosed as flo-powder) were determined on the basis of cell counts (initial algal concentration approximately 10E03 cells/ml).
- Reference substance (positive control):
- yes
- Remarks:
- phenol
Results and discussion
Effect concentrations
- Duration:
- 14 d
- Dose descriptor:
- EC50
- Effect conc.:
- ca. 3 g/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: estimated from the growth curves
- Details on results:
- A value of 1.5 g/L was estimated as concentration not resulting in a significant growth rate reduction. Here, the rising impact of turbidity on the results with increasing methenamine concentrations was taken into account, although this impact is not exactly quantifiable. Hence, the test is is considered not fully in line with OECD requirements. However, taking the findings of the other tests into account, the result is reliable and conservative enough for a risk assessment. The 14 day EC50 estimated from the growth curves is 3 g/L.
- Results with reference substance (positive control):
- Parallel test were conducted with phenol to give perspective as to the sensitivity of the assay procedure. These test gave positive results, as expected and demonstrated the sensitivity of the test system.
- Reported statistics and error estimates:
- Since all tests were run in triplicate, the values were averaged and expressed as log(10) numbers.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The LC50 of methenamine in Selenastrum capricornutum (freshwater) was determined to be about 3 g/L.
- Executive summary:
Selenastrum capricornutum was exposed in a 14 d growth inhibition test following U.S. EPA (AAP: BT) guidelines. Bold's mineral salts medium was slightly modified by adding tris buffer and NaHCO3 and an illumination regime of 12 h light/12 h dark was applied. The pH of the medium was 7.5. The maximum specific growth rates for 1, 2.5 and 5 g methenamine/l were determined on the basis of cell counts (initial algal concentration approximately 10E03 cells/mL).
A value of 1.5 g/L was estimated as concentration not resulting in a significant growth rate reduction. Here, the rising impact of turbidity on the results with increasing methenamine concentrations was taken into account, although this impact is not exactly quantifiable. Hence, the test is is considered not fully in line with OECD requirements. However, taking the findings of the other tests into account, the result is reliable and conservative enough for a risk assessment. The 14 day EC50 estimated from the growth curves is 3 g/L.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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