Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 205-769-8 | CAS number: 150-76-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to soil macroorganisms except arthropods
Administrative data
- Endpoint:
- toxicity to soil macroorganisms except arthropods: long-term
- Type of information:
- experimental study
- Adequacy of study:
- disregarded due to major methodological deficiencies
- Reliability:
- 3 (not reliable)
- Rationale for reliability incl. deficiencies:
- other: This study was not in accordance to the standardized method and the exact test concentrations could not be known due to the method of their preparation , the variability of solids in the sludge and the microbial activities.
Data source
Reference
- Reference Type:
- publication
- Title:
- Effect of aromatic compounds, humic acids and lignins on growth of the earthworm Eisenia foetida
- Author:
- Hartenstein R
- Year:
- 1 982
- Bibliographic source:
- Soil Biol Biochem, 14(6), 595-599.
Materials and methods
Test guideline
- Qualifier:
- no guideline followed
- Principles of method if other than guideline:
- The inhibition of the growth rate and the mortality of Eisenia Foetida were measured after 2 weeks
of exposure with the test substance in sludge. - GLP compliance:
- not specified
Test material
- Reference substance name:
- Mequinol
- EC Number:
- 205-769-8
- EC Name:
- Mequinol
- Cas Number:
- 150-76-5
- Molecular formula:
- C7H8O2
- IUPAC Name:
- 4-methoxyphenol
- Details on test material:
- The test substance was purchased from Aldrich Chemical Co, Milwaukee, WI and used without further purification.
Constituent 1
Sampling and analysis
- Analytical monitoring:
- no
- Details on sampling:
- not applicable
Test substrate
- Vehicle:
- not specified
- Details on preparation and application of test substrate:
- - Method of mixing into soil (if used): the test substances were processed for assay by mixing 0.01,
0.1, 0.5 or 1 g or ml into 100 g sludge which contained 11-15% solids.
- Method of application to soil surface (if used): About 30g sludge with test substance were placed
over a ca. 4mm depth of silt loam in a Petri dish.
- Controls: no data
Test organisms
- Test organisms (species):
- Eisenia fetida
- Animal group:
- annelids
- Details on test organisms:
- - Common name: Eisenia fetida
- Source: no data
- Age at test initiation (mean and range, SD): no data
- Weight at test initiation (mean and range, SD): each under 10 mg live, wt
ACCLIMATION
- Acclimation period: no data
- Acclimation conditions (same as test or not): no data
- Health during acclimation (any mortality observed): no data
Study design
- Study type:
- laboratory study
- Substrate type:
- other: Activated sludge
- Limit test:
- no
- Total exposure duration:
- 42 d
- Post exposure observation period:
- no data
Test conditions
- Test temperature:
- 24 +/- 1°C
- pH:
- no data
- Moisture:
- no data
- Details on test conditions:
- TEST SYSTEM
- Test container (material, size): Petri dish of 20 x 100 mm
- Amount of soil or substrate: about 30 g sludge (ca. 13% solids) were placed a ca. 4 mm depth of silt
loam
- No. of organisms per container (treatment): 2 hatchlings, each under 10 mg live, wt, were added to
petri dishes
- No. of replicates per treatment group: 5 replicates
- No. of replicates per control: 5 replicates
SOURCE AND PROPERTIES OF SUBSTRATE (if soil):
- sludge: sludge which contains 11-15% solids. No ther data.
- soil: silt loam. No other data.
OTHER TEST CONDITIONS
- Photoperiod: no data
- Light intensity: no data
EFFECT PARAMETERS MEASURED : Growth was checked every 2 wks.
Growth achieved at 2 or 4 and 6 weeks was determined by rinsing the worms in distilled water,
blotting and weighing. Weight at 6 weeks was evaluated by analysis of variance. Significant differences
were determined by the Neuman-Keul test. Mortality was determined by enumeration and said to be
significant if 5 or more earthworms died.
VEHICLE CONTROL PERFORMED: not applicable
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 5 and 10
- Justification for using less concentrations than requested by guideline: not applicable
- Range finding study: no data - Nominal and measured concentrations:
- The test substance, was processed for assay by mixing 0.01, 0.1, 0.5 or 1.0 g into 100 g sludge which
contained 11-15% solids. Assuming exactly 13% solids in the sludge, the substance was thus tested at
concentrations of about 0, 0.1, 1, 4 and 8% (w/w) dry wt, including controls. These figures are used in
the test, recognizing that the exact concentration could not be known. Solids are not homogeneously
ditributed in sludge and solids.Concentration varies from sludge sample to sample with time due to
microbial activies.The test substance concentrations were not measured at the end of the test and the
toxicity values were based on the nominal concentrations. Worms were transferred to freshly prepared
substance at 4 weeks. - Reference substance (positive control):
- not specified
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 42 d
- Dose descriptor:
- LC100
- Effect conc.:
- 40 other: g/kg dry weight
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Duration:
- 42 d
- Dose descriptor:
- LOEC
- Effect conc.:
- 10 other: g/kg dry weight
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth
- Details on results:
- GROWTH
Hatchlings in control dishes could gain more than 500 mg in 6 weeks.
An average wt of 582mg +/- 85 SD, with a coefficient of variation of 14.6%, was attained by controls in 6 weeks in 45
similar assays during 16 months. For PMP, the lowest amount which significantly reduced growth rate is 1%.
MORTALITY
Mortality among controls was less than 2%.
For PMP, the lowest amount which significantly caused death was 4% (100% mortality).
Results are reported in % (w/w) dry weight. - Results with reference substance (positive control):
- no data
- Reported statistics and error estimates:
- Significant differences were determined by the Neuman-Keul test.
Mortality was determined by enumeration and said to be significant if 5 or more earthworms died.
Any other information on results incl. tables
- Effect concentration: 4
- Unit of effect concentrations: % (w/w) dry weight
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.