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EC number: 265-060-4 | CAS number: 64741-59-9 A complex combination of hydrocarbons produced by the distillation of products from a catalytic cracking process. It consists of hydrocarbons having carbon numbers predominantly in the range of C9 through C25 and boiling in the range of approximately 150°C to 400°C (302°F to 752°F). It contains a relatively large proportion of bicyclic aromatic hydrocarbons.
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://echa.europa.eu/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_toxicological-information.png)
Acute Toxicity: inhalation
Administrative data
- Endpoint:
- acute toxicity: inhalation
- Type of information:
- migrated information: read-across based on grouping of substances (category approach)
- Adequacy of study:
- key study
- Study period:
- 1985-02-06 to 1985-06-06
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: This study is classified as reliable without restriction because it closely followed OECD guideline 403.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 986
- Report date:
- 1986
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 403 (Acute Inhalation Toxicity)
- GLP compliance:
- yes
- Test type:
- standard acute method
- Limit test:
- no
Test material
- Reference substance name:
- 64741-59-9
- Cas Number:
- 64741-59-9
- IUPAC Name:
- 64741-59-9
- Reference substance name:
- Light Catalytically Cracked Distillate
- IUPAC Name:
- Light Catalytically Cracked Distillate
- Test material form:
- other: low viscosity liquid hydrocarbon
- Details on test material:
- Test substance (as identified in study report): API 83-08 (CAS# 64741-59-9)
Physical description: brownish coloured liquid
Storage: refrigerated at 4°C
Gravity API: 24.2
Nitrogen, ppm: 312
Flash point: 142 °F
Viscosity at 40°C cST: 2.8
Paraffins 22.3%
Olefins 5.5%
Naphthenes 10.7%
Aromatics 61.5%
Saturates 33.0%
Distillation ASTM D86 Equivalent:
Initial Boiling Pt °F: 382
Boiling Pt Range (10-90%): 466-603
Final Boiling Pt °F: 642
Constituent 1
Constituent 2
Test animals
- Species:
- rat
- Strain:
- other: COBS CD (SD) BR
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Charles River Breeding Laboratories, Inc., Kingston, New York
- Age at study initiation: approximately 9 to 12 weeks old
- Weight at study initiation: approximately 282 to 373 grams for males and between 179 to 259 grams females
- Fasting period before study: not reported
- Housing: individually housed in stainless steel hanging wire cages
- Diet (e.g. ad libitum): ad libitum
- Water (e.g. ad libitum): ad libitum
- Acclimation period: at least 14 days
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 21 to 24°C
- Humidity (%): 50 to 68%
- Air changes (per hr): not reported
- Photoperiod (hrs dark / hrs light): 12 hours light/12 hours dark
IN-LIFE DATES: From: 1985-02-06 To: 1985-06-06
Administration / exposure
- Route of administration:
- inhalation: aerosol
- Type of inhalation exposure:
- whole body
- Vehicle:
- air
- Details on inhalation exposure:
- GENERATION OF TEST ATMOSPHERE / CHAMBER DESCRIPTION
- Exposure apparatus: chambers with square cross-sectional area and pyramidal top and base.
- Exposure chamber volume: 0.25 m3
- Method of holding animals in test chamber: not reported
- Source and rate of air: clean room air
- Method of conditioning air: not reported
- System of generating particulates/aerosols: DeVilbiss No. 40 nebulizer
- Method of particle size determination: gravimetrical determination
- Treatment of exhaust air: not reported
- Temperature, humidity, pressure in air chamber: 21 to 24°C, 50 to 68% relative humidity
TEST ATMOSPHERE
- Brief description of analytical method used: not reported; appendix not provided
- Samples taken from breathing zone: no
TEST ATMOSPHERE
Mass median aerodynamic diameter was within the range of 2.1 to 2.4 micrometres and geometric standard deviation was 1.63 to 1.77. - Analytical verification of test atmosphere concentrations:
- yes
- Duration of exposure:
- 4 h
- Concentrations:
- Nominal concentrations of 0, 2.5, 3.5, 5.0, 6.25, and 7.5 mg/L
Measured (time-weighted average) concentrations of 0, 2.34, 3.47, 5.06, 6.34 and 7.29 mg/L - No. of animals per sex per dose:
- 5 animals per sex per dose
- Control animals:
- yes
- Details on study design:
- - Duration of observation period following administration: 14 days
- Frequency of observations and weighing: All animals were checked twice daily for mortality. Animals were weighed prior to exposure and at days 7 and 14. Observation during exposure could not be completed due to decreased visibility as a result of aerosol production.
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weight,organ weights, histopathology, other: lungs and any other grossly abnormal tissues were removed and fixed for histological examination. - Statistics:
- Litchfield and Wilcoxon method was used to determine the LC50 value for the male data and the combined male and female data. Female data could not be fitted.
Results and discussion
Effect levelsopen allclose all
- Sex:
- male/female
- Dose descriptor:
- LC50
- Effect level:
- 4.65 mg/L air
- 95% CL:
- 3.89 - 5.55
- Exp. duration:
- 4 h
- Sex:
- male
- Dose descriptor:
- LC50
- Effect level:
- 4.65 mg/L air
- 95% CL:
- 4.22 - 5.13
- Exp. duration:
- 4 h
- Mortality:
- In males, mortality was observed in 0 of 5, 1 of 5, 0 of 5, 3 of 5, 3 of 5, and 5 of 5 rats at measured exposures of 0, 2.34, 3.47, 5.06, 6.34, and 7.29 mg/L, respectively. In females, mortality was observed in 0 of 5, 0 of 5, 1 of 5, 1 of 5, 5 of 5, and 5 of 5 animals at the measured exposures of 0, 2.34, 3.47, 5.06, 6.34, and 7.29 mg/L, respectively. These deaths occurred during the 14-day observation period and not during the course of the 4-hour exposure.
- Clinical signs:
- other: Animals displayed hair coat abnormalities (oily/wetness), crust around nose at 2 to 4 days post-exposure, skin abnormalities (scabs/flaky), hair loss, urine stain on coat, decreased activity/mobility, and eye abnormalities.
- Body weight:
- A dose-related suppression in the body weight gain was observed on day 7 and in animals surviving until the end of the 14-day observation period, especially males.
- Gross pathology:
- At necropsy, observations included dark red lungs and blood stains around nose and on coats in phase 1 animals. In four animals that died, congestion and oedema with extravasation of red blood cells were observed. Additionally, spotty necrosis of the bronchiolar epithelium was noted. Interstitial inflammation, focal alveolar histocytosis, and localised emphysema were noted in phase 2 animals. The histologic changes in animals that died were considered to be severe enough to have been the cause of death.
Any other information on results incl. tables
Table 1. Mean body weight data expressed as a percentage of day 0 weight.
Phase and Group Number | Measured Concentration Time-Weighted Average (mg/L) | Male | Female | ||
Day 7 | Day 14 | Day 7 | Day 14 | ||
PII-G1 | 0 | 111.2 | 120.4 | 107.0 | 113.2 |
PII-G2 | 2.34 | 99.6 | 113.4 | 105.2 | 108.4 |
PII-G4 | 3.47 | 100.4 | 111.4 | 100.1 | 105.8 |
PI-G1 | 5.06 | 99.7 | 116.4 | 99.3 | 111.5 |
PII-G5 | 6.34 | 92.1 | 103.2 | - | - |
PII-G3 | 7.29 | - | - | - | - |
Applicant's summary and conclusion
- Interpretation of results:
- harmful
- Remarks:
- Migrated information Criteria used for interpretation of results: EU
- Conclusions:
- The inhalation LC50 was determined to be 4.65 mg/L in males and females. The test material is classified as harmful by inhalation according to EU criteria.
- Executive summary:
In an acute inhalation toxicity study, groups of young adult COBS (CD) BR rats were exposed by inhalation route to light catalytically cracked distillate aerosol for 4 hours via whole-body exposure at measured concentrations of 0, 2.34, 3.47, 5.06, 6.34, or 7.29 mg/L. Animals then were observed for 14 days.
There were no deaths during the exposure period in any of the investigations, and macroscopic and microscopic findings were limited to the lungs, where moderate to severe pulmonary irritation was apparent. Clinical signs commonly included dyspnea and discharge from the nose and eyes. Decreased activity and mobility was reported at concentrations of 3.47 mg/L or 6.34 mg/L but not in male rats at 7.29 mg/L or either sex at 5.06 mg/L. During the observation period, mortality was observed in 0 of 5, 1 of 5, 0 of 5, 3 of 5, 3 of 5, and 5 of 5 male rats at measured exposures of 0, 2.34, 3.47, 5.06, 6.34, and 7.29 mg/L, respectively. In females, mortality was observed in 0 of 5, 0 of 5, 1 of 5, 1 of 5, 5 of 5, and 5 of 5 animals at the measured exposures of 0, 2.34, 3.47, 5.06, 6.34, and 7.29 mg/L, respectively. The inhalation LC50 was determined to be 4.65 mg/L (95% C.I.: 3.89 to 5.55 mg/L) in males and females, and 4.65 mg/L (95% C.I.: 4.22 to 5.13 mg/L) in males. The test material is classified as harmful by inhalation according to EU criteria.
This study received a Klimisch score of 1 and is classified as reliable without restriction because it closely followed OECD guideline 403.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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