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EC number: 436-060-3 | CAS number: - FC 84508 PK
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- From Dec. 05, 2000 to Jan. 04, 2001
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.3 (Algal Inhibition test)
- GLP compliance:
- yes
- Analytical monitoring:
- no
- Remarks:
- Considering the low aqueous solubility of the test substance (<1 mg/L), the test item concentration in medium was not verified analytically.
- Details on sampling:
- Not applicable
- Vehicle:
- yes
- Details on test solutions:
- No data
- Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
- TEST ORGANISM
- Strain: No data
- Source (laboratory, culture collection): Non-axenic strain of the test species obtained from 'The Collection of Algal Cultures' of the Institute of Plant
Physiology at the University of Goettingen (Germany)
- Method of cultivation: Exponentially-growing stock cultures are maintained in the test facility under constant temperature conditions
ACCLIMATION
- Culturing media and conditions (same as test or not): No
- Any deformed or abnormal cells observed: No - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 72 h
- Post exposure observation period:
- No
- Hardness:
- No data
- Test temperature:
- 21-25 °C
- pH:
- 7.4-10.3
- Dissolved oxygen:
- No data
- Salinity:
- No data
- Nominal and measured concentrations:
- 0, and 1 mg/L (nominal)
- Details on test conditions:
- TEST SYSTEM
- Renewal rate of test solution (frequency/flow rate): No
- Initial cells density: 10E+4 cells per mL in the test medium.
GROWTH MEDIUM
- Standard medium used: yes
TEST MEDIUM / WATER PARAMETERS
- The algal inocula for a test are taken from an exponentially-growing pre-culture and are mixed with the nutrient medium
OTHER TEST CONDITIONS
- Sterile test conditions: yes
- Adjustment of pH: yes
- Light intensity and quality: 60-120 µE × m-2 × S-1
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: Mean cell density, Percentage inhibition of cell growth and growth rate
- Range finding study
- Test concentrations: 0, and 1 mg/L (nominal) - Reference substance (positive control):
- no
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- > 1 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Details on results:
- - Exponential growth in the control (for algal test): yes
- Observation of abnormalities (for algal test): No abnormalities
- Effect concentrations exceeding solubility of substance in test medium: Test conducted at maximum water solubility.
No toxic effects were observed against algae at 1 mg/L (nominal). - Results with reference substance (positive control):
- Not applicable
- Reported statistics and error estimates:
- No data
- Validity criteria fulfilled:
- yes
- Conclusions:
- Under the test conditions employed, the 72 h EbC50, ErC50 and NOEC of the test substance were determined to be > 1 mg/L.
- Executive summary:
A study was performed to assess the adverse effects of the test substance on growth and growth rate of the planktonic freshwater algal species Scenedesmus subspicatus CHODAT over several generations. The study was conducted in accordance with EU method C.3 and OECD guideline 201 in compliance with GLP.
Exponentially growing algal cells were exposed for a period of 72 h to a limit test concentration of nominally 1 mg/L.
The test was conducted using dissolved test material at maximum water solubility. Auxiliaries used to prepare the test media, i.e. for maximisation of the dissolved fraction, and removal of undissolved material, were an ultrasonic bath, a magnetic stirrer and folded filter.
The cell densities were measured at 24 h intervals. Inhibition of the algal population was measured as reduction in growth (index b) and growth rate (index r), relative to the control.
No toxic effects against algae were observed at the limit test concentration of 1 mg/L (nominal).
Considering the low aqueous solubility of the test substance (< 1 mg/L) no specific analysis was established.
Under the employed test conditions, the 72 h EbC50, ErC50 and NOEC of the test substance were determined to be > 1 mg/L.
Reference
Table 1. Results
Concentration Nominal ( mg/L) |
Area under growth curve |
Inhibition (+) / Increase H(-) [%] |
|
Mean growth (b) [integral of biomass] |
0 |
611389 |
0.0 |
1 |
607778 |
0.6 |
|
Concentration Nominal ( mg/L) |
Growth rate |
Inhibition (+) / Increase H (-) [%] |
|
Mean growth rate (r) |
0 |
1.46 |
0.0 |
1 |
1.46 |
0.3 |
Description of key information
There is no adverse effects whatsoever of the test substance on algal biomass increase or growth rate at a nominal concentration of 1 mg/L, which resulted in the maximum attainable aqueous concentration (solubility limit). The NOEC of the test substance is therefore determined to be ≥ 1 mg/L. The 72 h EbC50 and ErC50 could not be determined but are hypothetically > 1 mg/L.
Key value for chemical safety assessment
Additional information
A study was performed to assess the adverse effects of the test substance on growth and growth rate of the planktonic freshwater algal species Scenedesmus subspicatus CHODAT over several generations. The study was conducted in accordance with EU method C.3 and OECD guideline 201 in compliance with GLP.
Exponentially growing algal cells were exposed for a period of 72 h to a limit test concentration of nominally 1 mg/L.
The test was conducted using dissolved test material at maximum water solubility. Auxiliaries used to prepare the test media, i.e. for maximisation of the dissolved fraction, and removal of undissolved material, were an ultrasonic bath, a magnetic stirrer and folded filter.
The cell densities were measured at 24 h intervals. Inhibition of the algal population was measured as reduction in growth (index b) and growth rate (index r), relative to the control.
No toxic effects against algae were observed at the limit test concentration of 1 mg/L (nominal).
Considering the low aqueous solubility of the test substance (< 1 mg/L) no specific analysis was established.
Under the employed test conditions, the 72 h EbC50, ErC50and NOEC of the test substance were determined to be > 1 mg/L.Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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