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EC number: 204-127-4 | CAS number: 116-15-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: inhalation
Administrative data
- Endpoint:
- acute toxicity: inhalation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study with acceptable restrictions
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 960
- Report date:
- 1960
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 403 (Acute Inhalation Toxicity)
- Deviations:
- yes
- GLP compliance:
- no
- Remarks:
- Pre-GLP
- Test type:
- standard acute method
- Limit test:
- no
Test material
- Reference substance name:
- Hexafluoropropene
- EC Number:
- 204-127-4
- EC Name:
- Hexafluoropropene
- Cas Number:
- 116-15-4
- Molecular formula:
- C3F6
- IUPAC Name:
- 1,1,2,3,3,3-hexafluoroprop-1-ene
- Reference substance name:
- 1-Propene, hexafluoro-
- IUPAC Name:
- 1-Propene, hexafluoro-
- Details on test material:
- Purity: 99.9% (Sample 1); 98.5% (Sample 2)
Constituent 1
Constituent 2
Test animals
- Species:
- rat
- Strain:
- other: Charles River CD
- Sex:
- male
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Age at study initiation: No data
- Weight at study initiation: No data
- Fasting period before study: No data
- Housing: Individually in stainless steel cages
- Diet (e.g. ad libitum): No data
- Water (e.g. ad libitum): No data
- Acclimation period: Rats were allowed 24 hours to become accustomed to the cages
ENVIRONMENTAL CONDITIONS
- Temperature (°C): No data
- Humidity (%): No data
- Air changes (per hr): No data
- Photoperiod (hrs dark / hrs light): No data
Administration / exposure
- Route of administration:
- inhalation: gas
- Type of inhalation exposure:
- whole body
- Vehicle:
- other: Air
- Details on inhalation exposure:
- GENERATION OF TEST ATMOSPHERE/CHAMBER DESCRIPTION:
- Exposure apparatus: Chamber was a cubical enclosure constructed of stainless steel and glass.
- Exposure chamber volume: 200- or 10000-liter-capacity chambers were used.
- Method of holding animals in test chamber: Animals were housed without food or water in two raised bottom cages made of galvanized screen. Five rats were placed in each cage, the dimensions of which were 9"X9"X15."
- System of generating particulates/aerosols: HFP was lead in copper tubing from the supply tank through a flow metering system of Fisher-Porter "Tri Flat" precison bore, flowrators and into a mixing manifold. Air was directed into the mixing manifold of the chamber through a high capacity rotometer which was rated at a maximum flow of 165 L/min. For the low flow rates used, a flowrator was employed whose maximum capacity was about 23 L/min. Flows of HFP and air were metered at a pressure slightly above one atmosphere on the intake side of the chamber which was consequently under positive pressure. The HFP-air mixture was exhausted from the chamber through a copper pipe. The exhaust of the chamber was accomplished by adjustment of the dampers in the exhaust ducts. The chamber was cleared of gases by opening the exhaust dampers and using an exhaust fan. When the 10000 L chamber was used, a mixing fan was also used in the chamber.
TEST ATMOSPHERE:
- Brief description of analytical method used: Spectrophotometric technique by adding piperidine and pyridine, and measuring at 420 microns. The concentration was computed from a previously derived calibration curve.
- Samples taken from breathing zone: No data. - Analytical verification of test atmosphere concentrations:
- yes
- Remarks:
- Atmosphere was sampled from the center of the chamber, dried w/sulfuricacid scrubbing units and absorbed by absolute methanol; contents of absorbers were placed into 200-mL flasks, liquid was measured spectrophotometrically at a wave length of 420 microns
- Duration of exposure:
- 4 h
- Concentrations:
- 3440 ppm; 3400 ppm; 3020 ppm; 2870 ppm; 2600 ppm; 2520 ppm; 2220 ppm; 1980 ppm; 1520 ppm; 1090 ppm; 690 ppm; 320 ppm; 140 ppm
- No. of animals per sex per dose:
- 10 male albino Charles River CD rats per exposure.
- Control animals:
- no
- Details on study design:
- Rats were allowed 24 hours to become accustomed to the cages; the following 24 hours urine was collected for measurements of volume and osmolality, and food and water consumption were determined. Post-exposure measurements where taken daily for 14 days after exposure and again for a 24-hour period at 3 and 4 weeks post-exposure for surviving rats.
- Statistics:
- LC50 was computed by the method of Litchfield JT and Wilcoxin F (1949) A Simplified Method of Evaluating Dose-Effect Experiments. J. Pharmacol. Experimental Therapeutics, 96:99.
Results and discussion
Effect levelsopen allclose all
- Sex:
- male
- Dose descriptor:
- LC50
- Effect level:
- 3 060 ppm
- 95% CL:
- 2 780 - 3 370
- Exp. duration:
- 4 h
- Remarks on result:
- other: Time of death varied from 2 to 12 days
- Sex:
- male
- Dose descriptor:
- other: NOAEL
- Effect level:
- 140 ppm
- Sex:
- male
- Dose descriptor:
- other: LOAEL (kidney morphology)
- Effect level:
- 320 ppm
- Remarks on result:
- other: Kidney function adverse effects at >320 ppm. Observed effects from 320 to 2600 ppm were predominantly healing (reversible) nephrosis. However, at concentrations of ≥2870 ppm, kidney effects were identified as nephrosis without evidence of reversibility.
- Mortality:
- Deaths were observed at concentrations of 2220 ppm and greater.
- Clinical signs:
- other: Animals showed pallor and discomfort during last hour at 2520 ppm to 3440 ppm exposure. Immediately following the exposures, animals were limp, weak, consumed little food, high urine volume, low urine osmolality and were very thirsty. Some animals had dia
- Body weight:
- Animals exposed above 320 ppm show retardation of weight gain. Rats which survived usually showed gains in weight by the tenth day after exposure at the latest.
- Gross pathology:
- At exposures above 140 ppm, animals showed nephrosis; kidney damage was seen in exposures of 320 ppm or greater.
- Other findings:
- Kidney function adverse effects seen between 320 and 690 ppm; kidney morphology effects were seen at 320 ppm. MIcroscopic examination of the tissues from rats exposed above 140 ppm disclosed that the major anatomical change was nephrosis. The lowest level at which an effect was observed was 320 ppm. At 140 ppm, the rats appeared normal in all respects.
Any other information on results incl. tables
|
Time of Survival (days) |
|
Pathology |
|||||||
Concentration (ppm) |
Chamber Size (L) |
Mortality |
Death |
Sacrifice |
Decreased Urine Osmolality |
Decreased Urine Volume |
Decreased Body Weight |
Kidney Weight (g) |
Kidney |
Other |
3440 |
10000 |
6/10 |
3-9 |
15 |
Not Det'd |
Not Det'd |
10/10 |
Not Done |
9/10 Nephrosis |
None |
3400 |
200 |
4/10 |
2-5 |
12 |
Not Det'd |
Not Det'd |
10/10 |
Not Done |
Not Det'd |
Not Det'd |
3020 |
10000 |
4/10 |
7-12 |
15 |
Not Det'd |
Not Det'd |
10/10 |
Not Done |
6/10 Nephrosis |
1/10 Myocarditis |
2870 |
200 |
8/10 |
4-9 |
14 |
Not Det'd |
Not Det'd |
10/10 |
4.34 |
10/10 Nephrosis |
2/10 Myocarditis |
2600 |
10000 |
0/10 |
- |
15 |
Not Det'd |
Not Det'd |
10/10 |
Not Done |
2/10 Nephrosis |
1/10 Broncho-pneumonia |
2520 |
200 |
2/10 |
5,10 |
17 |
Not Det'd |
Not Det'd |
10/10 |
4.61 |
8/10 Healing Neph. |
None |
2220 |
200 |
1/10 |
4 |
28 |
10/10 |
10/10 |
10/10 |
3.17 |
9/10 Healing Neph. |
2/10 Broncho-pneumonia |
1980 |
200 |
0/10 |
- |
14 |
Not Det'd |
Not Det'd |
10/10 |
3.71 |
10/10 Healing Neph. |
None |
1520 |
200 |
0/10 |
- |
28 |
10/10 |
10/10 |
10/10 |
3.61 |
5/10 Healing Neph. |
1/10 Pneumonitis |
1090 |
200 |
0/10 |
- |
28 |
Not Det'd |
10/10 |
10/10 |
3.65 |
10/10 Healing Neph. |
1/10 Focal Pneumonia |
690 |
200 |
0/10 |
- |
28 |
10/10 |
10/10 |
7/10slight |
3.22 |
7/10 Healing Neph. |
None |
320 |
200 |
0/10 |
- |
28 |
1/10 |
3/10 |
5/10 |
2.82 |
5/10 Healing Neph. |
1/10 Pneumonia |
140 |
200 |
0/10 |
- |
5,7 |
0/10 |
0/10 |
0/10 |
2.35 |
0/10 -0All NP |
None |
Applicant's summary and conclusion
- Conclusions:
- The 4-hour inhalation LC50 for male Charles River CD rats exposed to hexafluoropropene was determined to be 3060 ppm. HFP causes damage to the kidneys.
The study and the conclusions which are drawn from it fulfil the quality criteria (validity, reliability, repeatability). - Executive summary:
Rats were exposed via whole-body inhalation to 140, 320, 690, 1090, 1520, 1980, 2220, 2520, 2600, 2870, 3020, 3440 or 3440 ppm HFP for 4 hours. Animals were observed for clinical signs, body weight, food consumption, water consumption, urine volume, and urine osmolality for approximately two weeks post-exposure. A pathological exam was conducted at the end of the post-exposure period. The 4-hour inhalation LC50 for rats was found to be 3060 ppm. HFP caused damage to the kidneys.
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