Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 204-622-5 | CAS number: 123-35-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2010
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Remarks:
- Well described GLP study acording to OECD 301D guideline and giving reliable information on myrcene biodegradability.
- Reason / purpose for cross-reference:
- reference to same study
- Reason / purpose for cross-reference:
- reference to other study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test)
- Deviations:
- yes
- Remarks:
- ammonium chloride was omitted from the medium to prevent oxygen consumption due to nitrification/ activated sludge instead of effluent/ extract/mixture was used as inoculum
- GLP compliance:
- yes (incl. QA statement)
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): wastewater treatment plant Nieuwgraaf in Duiven, The Netherlands
- Pretreatment: the activated sludge was pre-conditionned to reduce the endogeous respiration rates. 400 mg dry weight/L of activated sludge was aerated for one week. - Duration of test (contact time):
- 28 d
- Initial conc.:
- 2 mg/L
- Based on:
- test mat.
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- TEST CONDITIONS
- Composition of medium: per liter of deionized water: 8.5mg KH2PO4, 21.75mg K2HPO4, 33.3mg Na2HPO4.2H2O, 22.5mg MgSO4.7H2O, 27.5mg CaCl2, 0.25mg FECl3.6H2O
- Additional substrate: silica gel
- Test temperature: within the prescribed temperature range of 22 to 24°C
- pH: 6.8 (control, control with silica), 6.9 (test)
- pH adjusted: no
- Continuous darkness: yes
TEST SYSTEM
- Number of culture flasks/concentration: 10 bottles containing only inoculum, 10 bottles containing inoculum and silica gel, 10 bottles containing inoculum and silica gel dosed with test substance, 6 bottles containing sodium acetate and inoculum
- Measuring equipment: an oxygen electrode (WTW TrioXmatic EO 200) and meter (WTW OXI 530) to electrochemically determine the dissolved oxygen concentrations, a Knick 765 calimatic pHmeter to measure pH, a sensor connected to a data logger to measure and record the temperature
- Test performed in closed vessels due to significant volatility of test substance
SAMPLING
- Sampling frequency: weekly
- Sampling method: Two duplicate bottles of all series were withdrawn for analyses of the dissolved oxygen concentration at day 7, 14, 21, and 28.
- Sample storage before analysis: the bottles were closed and incubated in the dark
CONTROL AND BLANK SYSTEM
- Inoculum blank: yes
- Abiotic sterile control: no
- Toxicity control: no inhibition of the biodegradation due to the "high" initial concentration of the test substance is expected - Reference substance:
- acetic acid, sodium salt
- Test performance:
- The validity of the test is demonstrated by an endogenous respiration of 1.0 mg/L at day 28. Sodium acetate was degraded 83% of its ThOD after 28 days. The validity of the test is also shown by oxygen concentrations >0.5 mg/L in all bottles during the test period.
- Key result
- Parameter:
- % degradation (O2 consumption)
- Value:
- 76
- Sampling time:
- 28 d
- Details on results:
- The pass level of 60% was reached after approximately 10 days upon achieving 10% biodegradation. Myrcene therefore fulfilled the 14-day time window (10-day for other OECD 301 tests) criterion for ready biodegradable compounds. The 14-day window is considered acceptable for the Closed Bottle test because the number of bottles to evaluate the 10-day window causes the test to become too unwieldy (OECD, 1992).
- Results with reference substance:
- See Tables in "any other information on results incl. tables section :
Table 1: Dissolved oxygen concentrations (mg/L) in the closed bottles.
Table 2: Oxygen consumption (mg/L) and the percentages biodegradation of the test substance, Myrcene (BOD/ThOD) and sodium acetate (BOD/ThOD) in the Closed Bottle test. - Validity criteria fulfilled:
- yes
- Interpretation of results:
- readily biodegradable
- Conclusions:
- Myrcene was readily biodegradable in a study conducted according to guideline OECD 301 D (biodegradation: 76% in 28 days).
- Executive summary:
A ready biodegradability study in closed bottles was conducted with substance myrcene according to guideline OECD 301 D.
The test item was introduced in closed bottles with preconditionned activated sludge (origin: wastewater treatment plant Nieuwgraaf in Duiven, NL). The closed bottles were incubated for 28 days at 23°C ± 1°C in the activated sludge and the oxygen consumption during this period was measured.
The BOD values were related to Theorical oxygen demand (ThOD) and percentage biodegradability in water was estimated.
Reference substance was Sodium acetate.
Oxygen consumption after 28 days of incubation was 5 mg O2/L test substance.
% BOD of ThOD after 28 days of incubation was 76%.
The test substance did not inhibit bacterial respiration at the tested concentration. Abiotic degradation was not recorded.
Therefore, it is considered that the test substance is readily biodegradable according to the criteria of guideline OECD 301D.
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Study period:
- 2007
- Reliability:
- 4 (not assignable)
- Rationale for reliability incl. deficiencies:
- documentation insufficient for assessment
- Remarks:
- Study conducted according to OECD Guideline 301 C but with deviations: no detail on material and methods
- Reason / purpose for cross-reference:
- reference to same study
- Reason / purpose for cross-reference:
- reference to other study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 C (Ready Biodegradability: Modified MITI Test (I))
- Deviations:
- yes
- Remarks:
- no detail on material and methods
- Principles of method if other than guideline:
- Not applicable
- GLP compliance:
- not specified
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge (adaptation not specified)
- Details on inoculum:
- Concentration of activated sludge : 30 ppm
- Duration of test (contact time):
- 14 d
- Initial conc.:
- 100 other: ppm
- Based on:
- test mat.
- Parameter followed for biodegradation estimation:
- O2 consumption
- Parameter:
- % degradation (O2 consumption)
- Value:
- >= 90 - <= 95
- Sampling time:
- 28 d
- Details on results:
- - Indirect analysis (BOD): 86%
- Direct analysis (TOC): 87%
- Direct analysis (GC): 99% - Validity criteria fulfilled:
- not specified
- Interpretation of results:
- readily biodegradable
- Conclusions:
- Under the test conditions, myrcene was readily biodegradable.
- Executive summary:
In a ready biodegradation study performed according to OECD Guideline 301 C, myrcene was tested at concentrations of 100 ppm and the inoculum was activated sludge (30 ppm). The degradation of the test material was assessed by the determination of the oxygen consumption.
At the test concentration of 100 ppm, 86% of degradation (biochemical oxygen demand) was reached in 14 days.
Therefore, myrcene was readily biodegradable.
Referenceopen allclose all
Table 1: Dissolved oxygen concentrations (mg/L) in the closed bottles.
Time (days) |
Oxygen concentration (mg/L) |
|||
Ocs |
Ot |
Oc |
Oa |
|
0 |
8.6 |
8.6 |
8.6 |
8.6 |
|
8.6 |
8.6 |
8.6 |
8.6 |
Mean |
8.6 |
8.6 |
8.6 |
8.6 |
7 |
7.8 |
4.9 |
8.0 |
3.9 |
|
8.0 |
5.3 |
7.9 |
3.9 |
Mean |
7.9 |
5.1 |
8.0 |
3.9 |
14 |
7.8 |
3.5 |
7.7 |
3.4 |
|
7.6 |
3.3 |
7.8 |
3.2 |
Mean |
7.7 |
3.4 |
7.7 |
3.3 |
21 |
7.7 |
2.8 |
7.8 |
|
|
7.6 |
2.9 |
7.6 |
|
Mean |
7.7 |
2.9 |
7.7 |
|
28 |
7.6 |
2.6 |
7.5 |
|
|
7.6 |
2.6 |
7.6 |
|
Mean |
7.6 |
2.6 |
7.6 |
|
Ocs Mineral nutrient solution without test material but with inoculum and silica gel.
Ot Mineral nutrient solution with test material (2.0 mg/L), silica gel, and inoculum.
Oc Mineral nutrient solution with only inoculum.
Oa Mineral nutrient solution with sodium acetate (6.7 mg/L) and with inoculum.
Table 2: Oxygen consumption (mg/L) and the percentages biodegradation of the test substance, Myrcene (BOD/ThOD) and sodium acetate (BOD/ThOD) in the Closed Bottle test.
Time (days) |
Oxygen consumption (mg/L) |
Biodegradation (%) |
||
Test substance |
Acetate |
Test substance |
Acetate |
|
0 |
0.0 |
0.0 |
0 |
0 |
7 |
2.8 |
4.1 |
42 |
76 |
14 |
4.3 |
4.5 |
65 |
83 |
21 |
4.8 |
|
73 |
|
28 |
5.0 |
|
76 |
|
None
Description of key information
Myrcene was found to be readily biodegradable in a GLP study conducted according to the criteria of the OECD 301 D guideline.
Key value for chemical safety assessment
- Biodegradation in water:
- readily biodegradable
Additional information
A test was carried out according to OECD 301D Ready biodegradability in closed bottles.
The test substance was incubated for 28 days at 23°C ± 1°C in closed bottles with preconditioned activated sludge and the oxygen consumption during this period was measured. % BOD of ThOD after 28 days of incubation was 76%.
In a ready biodegradation study performed according to OECD Guideline 301 C, myrcene was tested at concentrations of 100 ppm and the inoculum was activated sludge (30 ppm). The degradation of the test material was assessed by the determination of the oxygen consumption.
At a concentration of 100 ppm, 86% of degradation (biochemical oxygen demand) was reached in 14 days.
Therefore the test substance is readily biodegradable according to the criteria of the OECD 301 guideline.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.