Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 208-208-5 | CAS number: 515-74-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Additional toxicological data
Administrative data
- Endpoint:
- additional toxicological information
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Study period:
- September 20, 1988
- Reliability:
- 3 (not reliable)
- Rationale for reliability incl. deficiencies:
- other: Experimental result from a Bibliographic source.
Data source
Reference
- Reference Type:
- publication
- Title:
- Unnamed
- Year:
- 1 988
- Report date:
- 1988
Materials and methods
Test guideline
- Qualifier:
- no guideline followed
- Deviations:
- not applicable
Test material
- Reference substance name:
- Sodium sulphanilate
- EC Number:
- 208-208-5
- EC Name:
- Sodium sulphanilate
- Cas Number:
- 515-74-2
- Molecular formula:
- C6H7NO3S.Na
- IUPAC Name:
- sodium 4-aminobenzenesulfonate
Constituent 1
Results and discussion
Any other information on results incl. tables
The relative toxicity of the test compounds was determined by use of the PI50, which is the concentration of test compound required to induce a 50% reduction in cell protein content.
Results for 114 compounds are summarized in this publication. The compounds belong to many different chemical classes, and were presented in 7 groups. Within each group the compounds were arranged according to increasing PI50value. Surfactants and heavy metals both have very low PI50values. In all other groups a gradually increasing series is seen from low to high PIs0 values.
Citotoxicity related to sodium 4-aminobenzenesulfonate in terms of PI50(Concentration of test compound that induces a 50% decrease in cell proteins, determined after 24 hours of treatment) was calculated to be 97 mM.
Applicant's summary and conclusion
- Conclusions:
- Results for 114 compounds are summarized in this publication. The compounds belong to many different chemical classes, and were presented in 7 groups. Within each group the compounds were arranged according to increasing PI50 value. Surfactants and heavy metals both have very low PI50 values. In all other groups a gradually increasing series is seen from low to high PIs0 values.
Results for 114 compounds are summarized in this publication. The compounds belong to many different chemical classes, and were presented in 7 groups. Within each group the compounds were arranged according to increasing PI50 value. Surfactants and heavy metals both have very low PI50 values. In all other groups a gradually increasing series is seen from low to high PIs0 values.
Citotoxicity of 114 compounds has been assessed as an screening assay intended to produce information of the toxicity effect of the test substances. The results suggest that the assay could be a useful pre-screening method to test for the cytotoxicity of chemicals.Citotoxicity related to sodium 4-aminobenzenesulfonate in terms of PI50 (Concentration of test compound that induces a 50% decrease in cell proteins, determined after 24 hours of treatment) was calculated to be 97 mM. - Executive summary:
Supporting study:
An increasing demand for toxicity evaluation together with an increasing resistance to experiments using laboratory animals have led to the need for in vitro testing systems. Cytotoxicity studies can provide information about the potency and mechanism of action of xenobiotics, usually more rapidly and at lower cost than in vivo studies.
With the screening assay it was not intended to produce detailed information on mechanisms of toxicity, but rather to show a general biological effect that occurs after the living cells have come into contact with the test chemical.
The cellular protein content measured in cultured Hep G2 cells was used as the endpoint for determining the cytotoxicity of a range of 114 chemical compounds. The relative toxicity of the test compounds was quantified by the determination of the PI50, which is the concentration of xenobiotic required to produce a 50% reduction in protein content of the culture after 24 hours.
Surfactants and heavy metals consistently had low PIs0 values. Hep G2 cells were very sensitive to compounds with more than one carboxyl group. Triacetin and glutathione were identified as false positives. The results suggest that the PI50 assay could be a useful pre-screening method to test for the cytotoxicity of chemicals.
Results for 114 compounds are summarized in this publication. The compounds belong to many different chemical classes, and were presented in 7 groups. Within each group the compounds were arranged according to increasing PI50value. Surfactants and heavy metals both have very low PI50values. In all other groups a gradually increasing series is seen from low to high PIs0 values.
Citotoxicity related to sodium 4-aminobenzenesulfonate in terms of PI50(Concentration of test compound that induces a 50% decrease in cell proteins, determined after 24 hours of treatment) was calculated to be 97 mM.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.