Cinnamaldehyde

Toxicity to reproduction: other studies

Administrative data

Endpoint:

toxicity to reproduction: other studies

Type of information:

experimental study

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Data source

Reference

Reference Type:

publication

Title:

Fumigant Toxic Activity and Reproductive Inhibition Induced By Monoterpenes on Acanthoscelides obtectus (Say) (Coleoptera), a Bruchid of Kidney Bean (Phaseolus vulgaris L.)

Author:

CATHERINE REGNAULT-ROGER and ABDELAZIZ HAMRAOUI

Year:

1995

Bibliographic source:

J. srored Prod. Res. Vol. 31, No. 4, pp. 291-299, 1995

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: Principle mentioned below

Principles of method if other than guideline:

Combined Repeated, repro and developmental toxicity study of Cinnamaldehyde in 1 generation of A. Obtectus insects.

GLP compliance:

not specified

Type of method:

in vivo

Test material

Test material information

Constituent 1

Reference substance name:

Cinnamaldehyde

EC Number:

203-213-9

EC Name:

Cinnamaldehyde

Cas Number:

104-55-2

Molecular formula:

C9H8O

IUPAC Name:

3-phenylacrylaldehyde

Details on test material:

- Name of test material (as cited in study report): Cinnamaldehyde
- Molecular formula (if other than submission substance): C9-H8-O
- Molecular weight (if other than submission substance): 132.1612 g/mole
- Substance type: Organic
- Physical state: Liquid
- Impurities (identity and concentrations): No data avaialbe

Test animals

Species:

other: Insect

Strain:

other: Acanthoscelides obtectus (Coleoptera)

Sex:

male/female

Details on test animals or test system and environmental conditions:

Details on test animal
TEST ANIMALS
- Source: Cultivated own Institute.
- Age at study initiation: (P) x wks; (F1) x wks :No data avaialbe
- Weight at study initiation: (P) Males: x-x g; Females: x-x g; (F1) Males: x-x g; Females: x-x g: No data avaialbe
- Fasting period before study: No data avaialbe
- Housing: No data avaialbe
- Diet (e.g. ad libitum): No data avaialbe
- Water (e.g. ad libitum): No data avaialbe
- Acclimation period: No data avaialbe

ENVIRONMENTAL CONDITIONS
- Temperature (°C): 27°C
- Humidity (%):65-75%
- Air changes (per hr): No data avaialbe
- Photoperiod (hrs dark / hrs light): 12 h light/l2 h dark

Administration / exposure

Route of administration:

inhalation

Vehicle:

not specified

Details on exposure:

At the end of21 days, all adults were removed from the experimental chambers. Hatching larvae penetrated the seeds to continue development and the experiment was continued until the last emergence of the adults’ Fl progeny (about a 35-45 day period).

Mating procedure
- M/F ratio per cage: 1 : 1 ratio
- Length of cohabitation: 6 hours
- Proof of pregnancy: [vaginal plug / sperm in vaginal smear] referred to as [day 0 / day 1] of pregnancy No data available
- After ... days of unsuccessful pairing replacement of first male by another male with proven fertility. No data available
- Further matings after two unsuccessful attempts: [no / yes (explain)] No data available
- After successful mating each pregnant female was caged (how): No data available
Any other deviations from standard protocol:
Adult males and females were separated on the first day of emergence. The following day (24 h), pairs of beetles was set up and observed during the following 6 h. At the end of this time, only pairs known to have mated were chosen. Each treatment included 10 pairs (1 male and 1 fertilized female). Each pair was enclosed in an experimental chamber with six kidney beans and the small fumigant vessel prepared with chemicals.

Duration of treatment / exposure:

35-45 day period

Frequency of treatment:

Daily

Doses / concentrations

Remarks:

Doses / Concentrations:
0, 1.5 x 10-6 and 6 x 10-6 M (0, 0.0001989 and 0.0007929 mg/m3)
Basis:
no data

No. of animals per sex per dose:

Total: 60 insects
0 M: 10 males, 10 females
1.5 x 10-6 M: 10 males, 10 females
6 x 10-6 M: 10 males, 10 females

Control animals:

yes

Results and discussion

Effect levels

Dose descriptor:

NOAEL

Effect level:

0.001 mg/m³ air

Based on:

test mat.

Sex:

male/female

Applicant's summary and conclusion

Conclusions:

NOAEL was considered to be 0.0007929 mg/m3 when Acanthoscelides obtectus (Coleoptera) male and female insects treated with Cinnamaldehyde

Executive summary:

In a combined repeated and repro-development toxicity study,male and femaleAcanthoscelides obtectus (Coleoptera)insects were treated by inhalation withCinnamaldehydein the concentration of 0, 1.5 x 10-6M or 6 x 10-6M in an experimental chamber. All insects were observed for female fecundity, oviposition and the development of neonate and intracotyledonal larvae. No effect were observed on life span of adults, fecundity of female insects and oviposition of eggs as compared to control. In addition, no effect were observed on development of larvaes, larval penetration and adult emergence of treated insects as compared to control. Therefore, NOAEL was considered to be 6 x 10-6M whenmale and female Acanthoscelides obtectus (Coleoptera)insects were treated with Cinnamaldehydeby inhalation during a 35-45 day period.