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EC number: 202-876-1 | CAS number: 100-66-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Basic toxicokinetics
Administrative data
- Endpoint:
- basic toxicokinetics in vitro / ex vivo
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Study well described
Data source
Reference
- Reference Type:
- publication
- Title:
- Effets de l'anisole, de l'anéthole, du butylhydroxyanisole et du safrole sur le métabolisme de la flore caecale isolée, chez le rat
- Author:
- Fritsch P, de Saint-Blanquat G, Derache R, Canal T
- Year:
- 1 975
- Bibliographic source:
- European Journal of Toxicology, mai-juin 1975, 8, n°3, p. 169-174
Materials and methods
- Objective of study:
- metabolism
Test guideline
- Qualifier:
- no guideline followed
- Principles of method if other than guideline:
- The aim of this in vitro study was to assess the impact of 4 methoxybenzene substances including Anisole on the metabolism of the cecal flora in rats. Cecal flora was isolated from Wistar male rats and was incubated up to 60 minutes (10, 20, 30, 40, 50 and 60 minutes) with different concentrations of methoxybenzene substances (100, 200 and 400 µg/ml). A control without KOH was done and the effects of tween substances were examined.
- GLP compliance:
- no
- Remarks:
- before GLP statement
Test material
- Reference substance name:
- Anisole
- EC Number:
- 202-876-1
- EC Name:
- Anisole
- Cas Number:
- 100-66-3
- Molecular formula:
- C7H8O
- IUPAC Name:
- anisole
- Test material form:
- not specified
- Details on test material:
- no data
Constituent 1
- Radiolabelling:
- no
Test animals
- Species:
- rat
- Strain:
- Wistar
- Sex:
- male
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Weight at study initiation: ca. 280 g
- Fasting period before study: 17h
No more data.
Administration / exposure
- Route of administration:
- other: test on cecal flora directly
- Vehicle:
- other: Tween 20 at 1%
- Details on exposure:
- To isolate the cecal flora, wistar rats had a fasting period of 17 h, were sacrified by decapitation, at the same time of the day.
The cecal content was rapidly sampled and placed in 10 mL Krebs Ringer Phosphate (KRP), Ph 7.4 and 4°C.
A first centrifugation at 500 rpm during 2 mn eliminated food debris.
A second centrifugation at 4000 rpm during 10 mn (cold temperature), permitted the sedimentation of bacteria cells which were diluted in 5 mL of KRP, pH 7.4 and 4°C. After suspension of this media, aliquots were sampled in order to do incubation and dosage of DNA.
Metabolism study was realised with Warburg apparatus. All incubations were realised in strictly anaerobic conditions, with azote gas exempted of oxygen. The bacteria suspension was placed in buffered media containing 2% glucose.
The test items included Anisole were added at doses of 100, 200 and 400 µg/mL. Anisole was dispersed in Tween 20 at 1%.
Incubations were followed up to 60 mn and gaseous emission was reported to DNA of cecal bacteria, dosed according to the method of Dishes modified by Burton.
Controls were bacteria cells alone. - Duration and frequency of treatment / exposure:
- Not applicable
Doses / concentrations
- Remarks:
- Doses / Concentrations:
100, 200 and 400 µg/mL
- No. of animals per sex per dose / concentration:
- No data
- Control animals:
- other: bacteria cells coming from rat cecal flore
- Details on dosing and sampling:
- PHARMACOKINETIC STUDY (Absorption, distribution, excretion)
- Tissues and body fluids sampled (delete / add / specify): cecal flora
- Time and frequency of sampling: 1 time, just after sacrifice (fasting period of 17h)
- From how many animals: (samples pooled or not): no data - Statistics:
- Statistical comparison between controls and doses used were done by the couple test.
If necessary, regression curves were calculated (gaseous emission/time).
Results and discussion
Any other information on results incl. tables
Anisole was practically without effect on bacteria cecal flore coming from rats.
Validity of the experimental conditions was verified.
The couple test was employed, for each measured time.
The obtained value in the comparison system was never significant with Anisole.
Applicant's summary and conclusion
- Conclusions:
Anisole had no effect on the caecal flora in rats.- Executive summary:
In this study (1975), the authors assessed the effects of four methoxybenzene food additives (anisole, anethole, butylhydroxyanisole, safrole) on the metabolism of isolated cecal flora coming from rats (in vitro test).
Anisole had practically no effect on cecal bacteria activities at concentrations close to the dietary levels. In the contrary safrole, and especially butylhydroxyanisole, inhibited bacterial metabolism at the same doses. Anethol had similar effects compared to anisole.
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