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EC number: 200-554-5 | CAS number: 63-05-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 07 to 14 May 2013
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.1 (Acute Toxicity for Fish)
- Deviations:
- no
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Details on sampling:
- Sampling schedule:
- Stock solution: each day
- Control: in the freshly prepared media after 0 and 24 hours and in the medium after 24 hours of exposure
- Test concentration: in the freshly prepared media after 0 and 24 hours and in the media after 0 and 24 hours of exposure - Vehicle:
- no
- Details on test solutions:
- Test item concentration:
- 500 mg of the test item were added daily to 5 litres of dilution water, treated for 1 h in an ultrasonic bath and stirred for 1 h on a magnetic stirrer
- Undissolved particles were removed by filtration using a folded filter (pore size of 7-12 µm)
- pH: 7.8-8.0
- the test concentration was determined analytically by HPLC - Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- - Source: Aqua KloeGer (Germany)
- Date of birth: --
- Date supplied: 2013-04-09
- Acclimatisation: Stock held since 2013-04-09 and acclimatised to the test conditions since then
- Temperature: 20 - 24 °C
- Dissolved oxygen: > 5 mg/L
- Feeding: Commercial fish food, daily. Feeding discontinued 24 h prior to test start.
- Mortalities during acclimatisation period: < 5 %
- Medication: none
- Mean standard length (n = 42): 3.16 cm (S.D. = 0.24 cm) - Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Hardness:
- 14.4 °dH (257 mg/L CaCO3)
- Test temperature:
- 21.4-21.6 °C (after 96 hrs)
- pH:
- 7.9 (after 96 hrs)
- Dissolved oxygen:
- 8.5-8.7 mg/L (after 96 hrs)
- Salinity:
- n.a.
- Nominal and measured concentrations:
- nominal: 0.43, 0.94, 2.1, 4.5, 10 mg/L
Effective concentrations ranged from 89.3 % to 98.9 % % of nominal values at 0 hours and from 65.1 % to 89.5 % of nominal values at 24 hours under semi static conditions. - Details on test conditions:
- Exposure conditions
- Test vessels: glass aquaria holding 5 L of test media covered by glass plates
- Experimental design: 5 test concentrations plus 1 control
- 7 fish per test concentration
- no feeding during the exposure period
- Method of initiation: fish were placed in prepared media
- Loading: 0.46 g body weight (wet weight) per litre
- Photoperiod: 16 h light: 8 h dark
- Temperature: 21.4 to 22.2 °C
- Aeration: gentle aeration via narrow glass tubes
- Test item concentration: 0.43, 0.94, 2.10, 4.55 and 10 mg/L
- Method of administration: stock solution
- Medium renewal: daily
- Duration of exposure: 96 hours
- Criteria of effects: The criterion of death used in this study was the absence of response to physical stimulation. In addition to observations on mortality at 2, 24, 48, 72 and 96 hours, type and incidence of sub lethal effects compared with control fish were observed. - Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 8.609 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Details on results:
- All results are expressed in terms of geometric mean measured concentrations. Effective concentrations ranged from 89.3 % to 98.9 % % in the freshly prepared media and from 65.1% to 89.5 % in the media after 24 hours of exposure.
- Sublethal observations / clinical signs:
Validity criteriaaccording to OECD 203:
Target condition according to guideline: Actual condition according to the study: Validity criteria met: in the control(s) (dilution water control, solvent control), the mortality should
not exceed 10% (or one fish, if fewer than 10 control fish are tested) at the end
of the exposure
The mortality in the controls did not exceed 10 % by the end of the test. Yes the dissolved oxygen concentration should be ≥60% of the air saturation value
in all test vessels throughout the exposure
The dissolved oxygen concentration remained above 60 % of the air-saturation value throughout the exposure period. Yes The pH should not vary by more than one unit. The pH did not vary by more than 1 unit. Yes - Validity criteria fulfilled:
- yes
- Remarks:
- mortality in the controls < 10% by the end of the test; dissolved oxygen concentration above 60% of the air-saturation value throughout the exposure period; pH did not vary by more than 1 unit
- Conclusions:
- After 96 h a LC50 of 8.609 mg/l was measured for Androstendion towards fish. The result is expressed in terms of geometric mean measured concentrations. Effective concentrations ranged from 89.3 % to 98.9 % % in the freshly prepared media and from 65.1% to 89.5 % in the media after 24 hours of exposure.
- Executive summary:
The study was conducted under semi static conditions in accordance with Council Regulation (EC) No 440/2008, Method C.1 'Acute toxicity for Fish' (2008) which is in most parts equivalent to the OECD Guideline for Testing of Chemicals No. 203 'Fish, Acute Toxicity Test' (1992). Groups of seven fish of the recommended size were exposed to a range of concentrations, nominally 0.43, 0.94, 2.10, 4.55 and 10 mg/L of Androstendion dissolved in dilution water. Auxiliaries used to prepare the test media were an ultrasonic bath, a magnetic stirrer and folded filters. Observations were made on the numbers of dead fish and the incidence of sub-lethal effects after 2, 24, 48, 72 and 96 hours of exposure. This toxicity study is classified as acceptable and satisfies the guideline requirements for the acute fish study.
Reference
Description of key information
After 96 h a LC50 of 8.609 mg/l was determined. The result is expressed in terms of geometric mean measured concentrations.
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Dose descriptor:
- LC50
- Effect concentration:
- 8.609 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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