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EC number: 272-038-8 | CAS number: 68649-95-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- May 1988 - July 1988
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Not GLP, according to standard test method, but not in agreement with OECD 209.
- Qualifier:
- according to guideline
- Guideline:
- other: B. Robra, Wasser/Abwasser 117, 80 (1976)
- Deviations:
- no
- Principles of method if other than guideline:
- Pseudomonas putida cultures are exposed to the test substance for a period of 30 min to 3 hours. The oxygen consumption (in terms of mg/L/hour) is then measured and compared to that of the control. The percentage inhibition of the oxygen consumption is measured and EC values are estimated.
- GLP compliance:
- no
- Analytical monitoring:
- no
- Details on sampling:
- - Sampling method: not relevant, during this test the oxygen consumption is measured using e.g. an oxygen electrode
- Vehicle:
- yes
- Details on test solutions:
- The test solutions were prepared using an emulsifying agent: Laurylmyristylalcoholethoxylate 7 E.O
- Test organisms (species):
- Pseudomonas putida
- Test type:
- static
- Water media type:
- not specified
- Limit test:
- no
- Remarks on exposure duration:
- no data, the guideline prescribes a period of 30 min to 3 hours
- Hardness:
- not relevant
- Test temperature:
- no data
- pH:
- no data
- Dissolved oxygen:
- no data
- Salinity:
- no data
- Nominal and measured concentrations:
- Nominal concentrations: 80000; 90000; 95000 and 100000 mg/L
The concentrations were not verified by chemical analysis. - Details on test conditions:
- TEST SYSTEM
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): not reported
- No. of vessels per vehicle control (replicates): not reported
no further data available
EFFECT PARAMETERS MEASURED (with observation intervals if applicable)
Oxygen consumption was measured, the inhibition of oxygen consumption was calculated and the EC10 was determined - Reference substance (positive control):
- no
- Dose descriptor:
- EC0
- Effect conc.:
- 80 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Remarks on result:
- other: no data on the duration, the guideline prescribes a period of 30 min to 3 hours
- Dose descriptor:
- EC10
- Effect conc.:
- 90 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Remarks on result:
- other: no data on the duration, the guideline prescribes a period of 30 min to 3 hours; Based on the mean of 2 replicates
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Remarks on result:
- other: no data on the duration, the guideline prescribes a period of 30 min to 3 hours; Based on the mean of 2 replicates
- Details on results:
- - Effect concentrations exceeding solubility of substance in test medium: yes, because the solubility of the test material in water is <1 mg/L. However, an emulsifying agent was used and the guideline prescribes that the test solutions are aerated during the test, which enhances mixing.
- Validity criteria fulfilled:
- not specified
- Conclusions:
- Single species test with Pseudomonas putida:
EC10: 90 g/L
EC50: > 100 g/L - Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- May 1988 - July 1988
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Not GLP, according to standard test method, but not in agreement with OECD 209.
- Qualifier:
- according to guideline
- Guideline:
- other: B. Robra, Wasser/Abwasser 117, 80 (1976)
- Deviations:
- no
- Principles of method if other than guideline:
- Pseudomonas putida cultures are exposed to the test substance for a period of 30 min to 3 hours. The oxygen consumption (in terms of mg/L/hour) is then measured and compared to that of the control. The percentage inhibition of the oxygen consumption is measured and EC values are estimated.
- GLP compliance:
- no
- Analytical monitoring:
- no
- Details on sampling:
- - Sampling method: not relevant, during this test the oxygen consumption is measured using e.g. an oxygen electrode
- Vehicle:
- yes
- Details on test solutions:
- The test solutions were prepared using an emulsifying agent: Laurylmyristylalcoholethoxylate 7 E.O.
- Test organisms (species):
- Pseudomonas putida
- Test type:
- static
- Water media type:
- not specified
- Limit test:
- no
- Remarks on exposure duration:
- no data, the guideline prescribes a period of 30 min to 3 hours
- Hardness:
- no data
- Test temperature:
- no data
- pH:
- no data
- Dissolved oxygen:
- no data
- Salinity:
- no data
- Nominal and measured concentrations:
- Nominal concentrations: 5000; 10000; 30000; 40000; 50000 and 100000 mg/L
The concentrations were not verified by chemical analysis - Details on test conditions:
- TEST SYSTEM
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): not reported
- No. of vessels per vehicle control (replicates): not reported
No further data available
EFFECT PARAMETERS MEASURED (with observation intervals if applicable)
Oxygen consumption was measured, the inhibition of oxygen consumption was calculated and the EC10 was determined - Reference substance (positive control):
- no
- Dose descriptor:
- EC0
- Effect conc.:
- < 5 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Remarks on result:
- other: no data on the duration, the guideline prescribes a period of 30 min to 3 hours
- Dose descriptor:
- EC10
- Effect conc.:
- 6 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Remarks on result:
- other: no data on the duration, the guideline prescribes a period of 30 min to 3 hours
- Dose descriptor:
- EC50
- Effect conc.:
- ca. 25 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Remarks on result:
- other: EC50 not reported; estimated by the author of this summary from the reported graph of % inhibition versus test concentration; no data on the duration, the guideline prescribes a period of 30 min to 3 hours
- Details on results:
- - Effect concentrations exceeding solubility of substance in test medium: yes, because the solubility of the test material in water is <1 mg/L. However, an emulsifying agent was used and the guideline prescribes that the test solutions are aerated during the test, which enhances mixing.
- Validity criteria fulfilled:
- not specified
- Conclusions:
- Single species test with Pseudomonas putida:
EC10: 6 g/L
EC50: ca. 25 g/L - Endpoint:
- activated sludge respiration inhibition testing
- Data waiving:
- study scientifically not necessary / other information available
- Justification for data waiving:
- the study does not need to be conducted because the substance is found to be readily biodegradable and the applied test concentrations are in the range of concentrations that can be expected in the influent of a sewage treatment plant
Referenceopen allclose all
No data on the duration, the guideline prescribes a period of 30 min to 3 hours
No data on the duration, the guideline prescribes a period of 30 min to 3 hours.
Description of key information
At a concentration of 15.5 mg/L in the OECD 301B study it was shown that the substance does not inhibit microbial activity.
Key value for chemical safety assessment
- EC10 or NOEC for microorganisms:
- 15.5 mg/L
Additional information
Two studies are available for Pseudomona putida. An emusifier is used to prepare the test solutions. The test substance that show toxic effects at extremely high concentrations with the lowest EC10 = 6 g/L and lowest EC50 = 25 g/L. This study is considered to be in line with OECD 209. However, a more representative value is derived from the biodegradation study (OECD 301B) where the substance did not inhibit the microbial activity at 15.5 mg/L.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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