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Diss Factsheets
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EC number: 202-501-1 | CAS number: 96-34-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: No GLP and method similar to OECD 471.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 983
- Report date:
- 1983
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Deviations:
- no
- GLP compliance:
- no
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- Methyl chloroacetate
- EC Number:
- 202-501-1
- EC Name:
- Methyl chloroacetate
- Cas Number:
- 96-34-4
- Molecular formula:
- C3H5ClO2
- IUPAC Name:
- methyl 2-chloroacetate
- Details on test material:
- Name of test material (as cited in study report): Monochloressigsäuremethylester
- Physical state: Colourless liquid
- Analytical purity: 99.4%
- Lot/batch No.: KW 0727267-5 vom 28.10.82
- Storage condition of test material: Dark at 20ºC
Constituent 1
Method
Species / strain
- Species / strain / cell type:
- other: Salmonella typhimurium TA 100, TA 98, TA 1535, TA 1537, TA 1538 and Escherichia coli WP2uvrA
- Metabolic activation:
- with and without
- Metabolic activation system:
- S-9 mix
- Test concentrations with justification for top dose:
- 0, 4, 20, 100, 500, 2500 and 5000 µg/plate (dissolved in 100 µl DMSO)
- Vehicle / solvent:
- - Vehicle(s)/solvent(s) used: DMSO
Controlsopen allclose all
- Negative solvent / vehicle controls:
- yes
- Positive controls:
- yes
- Positive control substance:
- sodium azide
- Remarks:
- without metabolic activation: TA100 and TA1535
- Positive controls:
- yes
- Positive control substance:
- 9-aminoacridine
- Remarks:
- without metabolic activation: TA1537
- Positive controls:
- yes
- Positive control substance:
- 2-nitrofluorene
- Remarks:
- without metabolic activation: TA98 and TA1538
- Positive controls:
- yes
- Positive control substance:
- other: N-methyl-N´-nitro-N-nitrosoguanidine
- Remarks:
- without metabolic activation: WP2uvrA
- Positive controls:
- yes
- Positive control substance:
- benzo(a)pyrene
- Remarks:
- with metabolic activation: TA98, TA100, TA1535, TA1537, TA1538 and WP2uvrA
- Positive controls:
- yes
- Positive control substance:
- other: 2-aminoanthracene
- Remarks:
- with metabolic activation: TA98, TA100, TA1535, TA1537, TA1538 and WP2uvrA
- Details on test system and experimental conditions:
METHOD OF APPLICATION: in agar (plate incorporation);
DURATION
- Exposure duration: 48 to 72 hours at 37 ºC in the dark
NUMBER OF REPLICATIONS: Triplicates
DETERMINATION OF CYTOTOXICITY
- Method: Thinning of the bacterial lawn and reduction on the number of colonies. The solvent control is compared with the number of colonies per plate in the presence of the test compound (surviving factor).
Results and discussion
Test results
- Species / strain:
- other: Salmonella typhimurium TA98, TA100, TA1535, TA1537, TA1538 and E. coli WP2uvrA
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- At doses of 2500 µg/plate
- Vehicle controls validity:
- valid
- Positive controls validity:
- valid
- Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Any other information on results incl. tables
The test compound did not cause a significant increase in the number of revertant colonies with any of the tester strains neither in the absence nor presence of S9-mix. It is concluded that the test substance is not mutagenic in this bacterial test system neither in the absence nor in the presence of metabolic activation.
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
negative
The test compound did not cause a significant increase in the number of revertant colonies with any of the tester strains neither in the absence nor presence of S9-mix. It is concluded that the test substance is not mutagenic in this bacterial test system neither in the absence nor in the presence of metabolic activation. - Executive summary:
The test substance methyl chloroacetate was tested for mutagenicity with the strains TA98, TA100, TA1535, TA1537 and TA1538 of Salmonella typhimurium and WP2uvrA of E.coli. The mutagenicity studies were performed in the absence and in the presence of metabolic activation. A dose range of six concentrations from 4 µg/plate to 5000 µg/plate was used. Control plates and positive controls were also included in the study. The test compound did not cause a significant increase in the number of revertant colonies with any of the tester strains neither in the absence nor presence of S9-mix. It is concluded that the test substance is not mutagenic in this bacterial test system neither in the absence nor in the presence of metabolic activation.
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