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EC number: 212-751-3 | CAS number: 866-81-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Endpoint summary
Administrative data
Key value for chemical safety assessment
Additional information
There are no data available on genetic toxicity of Tricobalt dicitrate. However, there are reliable data available for structurally related cobalt compounds. Thus, read-across was conducted based on structural analogues.
In vitro
Soluble cobalt salts were found to be generally nonmutagenic in Salmonella typhimurium strains when compounds with a valence state of II were tested (Zeiger et al., 1992; Ogawa et al., 1986; Tso and Fung, 1981). However, Ogawa et al. (1986) could show that when the Ames test was performed with 9-aminoacridine in the presence of varying concentrations of cobalt(II)chloride, a remarkable increase in the mutagenic activity was observed in TA1537 and TA2637. Furthermore cobalt(II)chloride could induce mutation when combined with 4 -aminoquinoline, harman or norharman, which were not themselves mutagenic in the strains TA1537 or TA2637.
In mouse bone marrow cells, micronucleus formation was not significantly altered by treatment with cobalt(II)chloride hexahydrate in the presence or absence of S9 mix (Suzuki et al., 1993). However, no positive control substance was used to validate this assay.
In vivo
30 hours following single intraperitoneal injection of cobalt(II)chloride hexahydrate in mice, a dose-dependent increase in micronucleus formation was seen at 69.7 and 38.7 mg/kg bw, but not at 19.4 mg/kg bw Tricobalt dicitrate (recalculated values, equivalent to 25, 50 and 90 mg/kg bw cobalt(II)chloride hexahydrate) (Suzuki et al., 1993).
Reference:
Tso, WW and Fung, WP, 1981, Mutagenicity of metallic cations. Toxicology Letters, 8: 195 -200
Short description of key information:
In vitro: Negative results in the Ames test and in the micronucleus assay. However, cobalt(II)chloride was identified as comutagen in the Ames test.
In vivo: Positive result in the micronucleus assay.
Endpoint Conclusion:
Justification for classification or non-classification
There are no data available on genetic toxicity of Tricobalt dicitrate. However, there are reliable data available for structurally related cobalt compounds. Thus, read-across was conducted based on structural analogues.
If there are positive results in at least one valid in vivo mutagenicity test using intraperitoneal application, or from at least one valid in vivo genotoxicity test using intraperitoneal application plus supportive in vitro data, classification is warranted. In this case all in vitro tests were negative. It cannot be ruled out that a positive test result in intraperitoneal studies in rodents only may be relevant to humans. But because of the absorption of cobalt salts after orally intake, Tricobalt dicitrate has to be classified.
DSD: Mutagenicity Category 3, R68
CLP: Mutagenicity Category 2
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