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EC number: 237-487-6 | CAS number: 13814-97-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- migrated information: read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Study period:
- 1986-11-10 to 1986-11-24
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: GLP accreditated laboratory. Result negative but no independet repeats followed.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 987
- Report date:
- 1987
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Deviations:
- no
- GLP compliance:
- yes
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- Tin(II) bis(methanesulfonate)
- EC Number:
- 401-640-7
- EC Name:
- Tin(II) bis(methanesulfonate)
- IUPAC Name:
- tin(II) bis(methanesulfonate)
- Reference substance name:
- 53408-94-9
- EC Number:
- 610-996-4
- Cas Number:
- 53408-94-9
- IUPAC Name:
- 53408-94-9
- Test material form:
- solid: particulate/powder
- Remarks:
- migrated information: powder
- Details on test material:
- - Name of test material (as cited in study report): Tin (II) methanesulfonate
- Physical state: white powder
- Storage condition of test material: at room temperature, light protected
Constituent 1
Constituent 2
Method
- Target gene:
- not applicable
Species / strain
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Metabolic activation:
- with and without
- Metabolic activation system:
- S9-mix (rat-liver homogenate (Aroclor induced); lot umber 07415; protein content - 35.7 mg/mL)
- Test concentrations with justification for top dose:
- Preliminary toxicity determination: 0, 19, 39, 78, 156, 312, 625, 1250, 2500 and 5000 µg/plate
Experiment 1: 0, 8, 40, 200, 1000 and 5000 µg/plate (with and without metabolic activation)
Experiment 2: 0, 61.7, 185, 555, 1666 and 5000 µg/plate (with and without metabolic activation) - Vehicle / solvent:
- - Vehicle(s)/solvent(s) used: A. dest.
50 mg of tin (II) methanesulfonate was dissolved in 1 mL A. dest.
Controlsopen allclose all
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- Remarks:
- A. dest.
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- sodium azide
- Remarks:
- Positive control: without metabolic activation; 0.25 µg/plate for strains TA 100 and TA 1535
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- Remarks:
- A. dest.
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- 2-nitrofluorene
- Remarks:
- Positive control: without metabolic activation; 5 µg/plate for strain TA 98
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- Remarks:
- A. dest.
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- 9-aminoacridine
- Remarks:
- Positive control: without metabolic activation; 50 µg/plate for strains TA 1537
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- Remarks:
- A. dest.
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- other: 2-Aminoanthracene
- Remarks:
- Positive control: with metabolic activation; 2.00 µg/plate for strains TA 1535 and TA 1537; 0.25 µg/plate for strains TA 100 and TA 98
- Details on test system and experimental conditions:
- METHOD OF APPLICATION: in agar (plate incorporation)
DURATION
- Exposure duration: 72 hours
NUMBER OF REPLICATIONS: 3
NUMBER OF CELLS EVALUATED:
The number of his+ revertants was counted and recorded.
PRELIMINARY TOXICITY DETERMINATION:
To know from which concentration on a compound shows toxicity for the tester strains, before mutagenicity testing, the survival, spontaneous reversion and the inhibition of the background lawn of auxotrophic cells of one of the strains (TA 100) is determined at different concentrations of the compound.
Bacterial cells are exposed to different concentrations of the compound and after incubation the background lawn and the number of spontaneous revertants are examined.
Parallel appropriate (10^-6) dilutions of the cells are plated on NB agar plates and the influence of the compound over the survival of the cells is determined. - Evaluation criteria:
- A compound will be considered to be mutagenic if the following criteria are fullfilled: if the his+ revertants of the solvent control lie in the normal range, at least for one strain a dose response effect must exist and the greatest increase of the revertant colonies has to be two times higher than the solvent control. The result has to be reproducible.
Results and discussion
Test results
- Species / strain:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity nor precipitates, but tested up to recommended limit concentrations
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not examined
- Positive controls validity:
- valid
- Additional information on results:
- PRELIMINARY TOXICITY DETERMINATION:
The test compound did not show toxicity up to a concentration of 5000 µg per plate.
Therefore the mutagenicity experiments were performed in a concentration range from 8 µg to 5000 µg (1. experiment) resp. 61.7 µg to 5000 µg (2.experiment) per plate.
MUTAGENICITY EXPERIMENTS:
The controls without mutagen showed a normal number of revertants
The positive controls demonstrated the sensibility of the indicator strains and the activity of the metabolising system. The sterility controls of top agar, S9-mix and test compound showed no growth.
The results of the mutagenicity experiments with the test compound tin (II) methanesulfonate indicate, that in the tested concentration range with and without metabolic activation no significant increase of his+ revertants over the spontaneous values could be detected with the tester strains TA100, TA1535, TA98 and TA1537.
Please also refer for results to the field "Attached background material" below. - Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'. Remarks: TA100, TA1535, TA98, TA1537
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
negative
It is concluded that tin (II) methanesulfonate showed no evidence of mutagenic activity in this bacterial system under the test conditions employed.
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