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EC number: 700-393-5 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2008-10-08 to 2009-01-15
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 009
- Report date:
- 2009
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Deviations:
- no
- GLP compliance:
- yes
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- Reaction mass of biphenyl-2-yl diphenyl phosphate and triphenyl phosphate and bis(biphenyl-2-yl) phenyl phosphate
- EC Number:
- 700-393-5
- IUPAC Name:
- Reaction mass of biphenyl-2-yl diphenyl phosphate and triphenyl phosphate and bis(biphenyl-2-yl) phenyl phosphate
- Details on test material:
- - Name of test material (as cited in study report): #5 Biphenyl diphenyl phosphate (mixture of the phosphates of ortho-Phenyl Phenol and Phenol and containing 25 % of Triphenyl Phosphate)
- Substance type: organic
- Physical state:Colorless to light yellowish clear liquid
- Analytical purity: > 99 %
- Impurities (identity and concentrations): no data
- Lot/batch No.: F01003
- Expiration date of the lot/batch: 2009-03-15
- Stability under test conditions: no data
- Storage condition of test material: room temperature; store in a cool, dry, well-ventilated area; keep containers tightly closed when not in use.
Constituent 1
Method
- Target gene:
- not applicable
Species / strain
- Species / strain / cell type:
- S. typhimurium, other: TA97a, TA98, TA100, TA102 and TA1535
- Details on mammalian cell type (if applicable):
- not applicable
- Additional strain / cell type characteristics:
- not specified
- Metabolic activation:
- with and without
- Metabolic activation system:
- S9
- Test concentrations with justification for top dose:
- 5, 2.5, 1.25, 0.625 and 0.3125 µL/plate
Controls
- Untreated negative controls:
- yes
- Remarks:
- sterile water
- Negative solvent / vehicle controls:
- no
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- other: different positive control substances were used - please see Table 2.1 and 2.2 in "Any other information on materials and methods incl. tables"
Results and discussion
Test results
- Species / strain:
- S. typhimurium, other: TA97a, TA98, TA100, TA102 and TA1535
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Vehicle controls validity:
- not examined
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Any other information on results incl. tables
1) Evaluation of test results
- The raw data of spontaneous revertants values were represented with Mean ± S.D.
- The raw data were analyzed by ANOVA to evaluate the difference between negative control group and test article groups, and p <0.05 indicates the significant difference.
- A increase in revertants over the negative control was as the cut-off between a mutagenic and non-mutagenic response:
A. TA97a, TA98, TA100 and TA102: had more than two-fold increase in revertants over the negative control, then the test articles were considered a mutagen.
B. TA1535: had more than three-fold increase in revertants over the negative control, then the test articles were considered a mutagen.
2) Results
a) Result of genotyping of Salmonella typhimurium strains
The genotype of five Salmonella typhimurium strains (TA97a, TA98, TA100, TA102 and TA1535) for this study were identified and met the standard as described in Table 1:
Table 1. Result of Genotyping of Salmonella typhimurium strains
Salmonella typhimurium strains | Histidine requirement | UV sensitivity | Crystal violet sensitivity | Ampicillin resistance | Tetracycline resistance | Spontaneous revertants | |
His+ Bio- Plate |
His- Bio- Plate |
His+ Bio- Plate | Zone of growth inhibition* | Ampicillin plate | Ampicillin Tetracycline plate | without S9 | |
TA97a | + | — | — | + | + | — | 143.0 ± 6.1 |
TA98 | + | — | — | + | + | — | 12.7 ± 2.1 |
TA100 | + | — | — | + | + | — | 95.0 ± 5.0 |
TA102 | + | — | + | + | + | + | 127.0 ± 9.5 |
TA1535 | + | — | — | + | — | — | 16.0 ± 1.0 |
* +: a clear zone of inhibition appeared around the disc
b) Result of dose range finding of test article in TA100 and bacterial reverse mutation test
- The C.V. of 3 plates/group for the negative control group, positive control group and testing groups were met the criterion.
- Based on the result of dose range finding, the concentrations of 5, 2.5, 1.25, 0.625 and 0.3125 uL/plate of "#5 Biphenyl diphenyl phosphate" were used for bacterial reverse mutation test in five Salmonella typhimurium strains. The positive control articles were caused more than twofold increase in revertant colonies on TA97a, TA98, TA100 and TA102; more than three-fold increase in revertants colonies on TA1535 (Table 3).
- In all five Salmonella typhimurium strains, there were no significant increase in the numbers of revertant colonies in the presence and absence of a S9 mixture metabolic activation system (Table 3).
Table 2. Result of Dose Range Finding of Test Article in Salmonella typhimurium TA100
Dose (µL /plate) | Number of revertants / plate (Mean ± S.D., n=3) |
TA100 | without S9 mixture |
Negative control (Sterile water) | 111.0 ± 13.1 |
Positive control | 523.3 ± 74.6 |
#5 Biphenyl diphenyl phosphate (CAS number: 132-29-6) | |
5 | 110.0 ± 10.5 |
2.5 | 110.0 ± 11.4 |
1.25 | 111.0 ± 10.6 |
0.625 | 106.3 ± 9.3 |
0.3125 | 115.3 ± 9.6 |
Table 3. Result of Bacterial Reverse Mutation Test
Dose (µL /plate) | Number of revertants /plate (without S9 mixture, Mean ± S.D., n=3) | ||||
TA97a | TA98 | TA100 | TA102 | TA1535 | |
Negative control (sterile water) | 146.7 ± 0.6 | 13.0 ± 3.5 | 108.3 ± 16.9 | 145.3 ± 13.6 | 13.3 ± 0.6 |
Positive control | 570.7 ± 78.9 | 159.3 ± 19.0 | 437.3 ± 28.1 | 640.0 ± 36.7 | 298.7 ± 20.1 |
#5 Biphenyl diphenyl phosphate (CAS number: 132-29-6) | |||||
5 | 182.3 ± 14.4 | 12.0 ± 1.7 | 129.7 ± 5.8 | 139.3 ± 9.0 | 12.0 ± 3.0 |
2.5 | 171.3 ± 6.7 | 13.0 ± 1.0 | 123.3 ± 15.3 | 136.0 ± 13.9 | 15.7 ± 3.1 |
1.25 | 178.3 ± 2.5 | 10.7 ± 0.6 | 107.3 ± 5.7 | 150.0 ± 4.0 | 15.3 ± 3.2 |
0.625 | 163.0 ± 7.9 | 11.7 ± 1.5 | 115.7 ± 13.7 | 154.7 ± 4.6 | 14.0 ± 2.0 |
0.3125 | 174.7 ± 4.9 | 11.7 ±2.1 | 122.3 ± 10.7 | 154.0 ± 12.5 | 12.7 ±3.2 |
Dose (µL /plate) | Number of revertants /plate (with S9 mixture, Mean ± S.D., n=3) | ||||
Negative control (sterile water) | 186.3 ± 13.0 | 14.3 ± 3.5 | 95.7 ± 4.0 | 123.3 ± 3.1 | 13.7 ± 3.5 |
Positive control | 1920.0 ± 80.0 | 430.7 ± 48.1 | 480.0 ± 40.0 | 608.0 ± 21.2 | 180.0 ± 12.5 |
#5 Biphenyl diphenyl phosphate (CAS number: 132-29-6) | |||||
5 | 192.0 * 7.9 | 16.7 ± 2.5 | 105.3 ± 11.9 | 130.0 ± 7.2 | 12.0 ± 1.7 |
2.5 | 198.3 ± 4.2 | 19.7 ± 2.9 | 119.0 ± 9.5 | 126.0 ± 7.2 | 12.3 ± 4.0 |
1.25 | 193.0 ± 1.0 | 16.7 ± 3.5 | 95.7 ± 9.8 | 142.0 ± 5.3 | 9.7 ± 0.6 |
0.625 | 197.3 ± 6.5 | 21.0 ± 0.0 | 94.0 ± 4.4 | 158.0 ± 7.2 | 9.3 ± 0.6 |
0.3125 | 206.7 ± 11.6 | 18.7 ± 2.1 | 104.0 ± 9.5 | 153.3 ± 11.7 | 12.7 ± 2.1 |
Table 4. Concentration of positive control
Strain | S9 mixture | Positive control | Concentration (µg /plate) |
TA97a | without | 9-Aminoacridine (9-AA) | 50 |
TA98 | 2-nitrofluorene (2-NF) | 1 | |
TA100 | sodium azide (SA) | 1 | |
TA102 | mitomycin C (MMC) | 0.2 | |
TA1535 | sodium azide (SA) | 1 | |
TA97a | with | 2-aminoanthracene (2-AA) | 5 |
TA98 | 2-aminoanthracene (2-AA) | 1 | |
TAIOO | benzo(a)pyrene (B(a)P) | 1 | |
TA102 | 2-aminoanthracene (2-AA) | 5 | |
TA1535 | 2-aminoanthracene (2-AA) | 5 |
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
negative with metabolic activation
negative without metabolic activation
Based on the results of bacterial reverse mutation test, for five Salmonella typhimurium strains, no significant increase in the numbers of revertant colonies were observed in the presence and absence of a S9 mixture metabolic activation system.
In conclusion, the test item did not cause mutagenicity in this study. - Executive summary:
"Bacterial Reverse Mutation Test" was used in this study to evaluate the genotoxicity of "#5 Biphenyl diphenyl phosphate". The test was performed in accordance with OECD Guideline 471.
Five doses (5, 2.5, 1.25, 0.625 and 0.3125 µL /plate) were used for dose range finding test in Salmonella typhimurium TA100 to determinate the testing dosage of bacterial reverse mutation test. The results showed that the test article was non-cytotoxicity and five doses (5, 2.5, 1.25, 0.625 and 0.3125 µL/plate) were used for bacterial reverse mutation test in Salmonella typhimurium TA97a, TA98, TA100, TA102 and TA1535. Plate incorporation method was used in the presence and absence of a S9 mixture metabolic activation system.
Based on the results of bacterial reverse mutation test, for -fiveSalmonella typhimuriumstrains, no significant increase in the numbers of revertant colonies were observed in the presence and absence of a S9 mixture metabolic activation system. In conclusion, "#5 Biphenyl diphenyl phosphate" did not cause mutagenicity in this study.
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