Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 230-257-6 | CAS number: 6990-43-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Neurotoxicity
Administrative data
Description of key information
Key value for chemical safety assessment
Additional information
A study report on a neurotoxic esterase (NTE) assay is available for zinc O,O,O',O'-tetrabutyl bis(phosphorodithioate). However, the composition of the reported test material is unclear and the impurity profile is not specified. It is thus questionable, whether the results obtained were related to the test substance as defined in the substance identity profile or to unidentified impurities in the actual test material.
In this study, the potential inhibitory effect of zinc O,O,O',O'-tetrabutyl bis(phosphorodithioate) on NTE activity was investigated in White Leghorn hens (Richardson, 1985). A group of 6 animals received a single oral dose of the test material at 2000 mg/kg bw in a gelatine capsule. Two further groups receiving corn oil (2000 mg/kg bw) and tri-o-cresylphosphate (750 mg/kg bw) served as negative and positive control groups, respectively. After 24 h, animals were sacrificed, their brains were prepared, weighed and homogenised for the biochemical assay.
The NTE assay was performed according to the protocol described in Johnson (1977). Shortly, the amount of NTE activity present in diluted whole hen brain homogenate is determined by a colourimetric reaction in which 4-amino-antipyrine (AAP) reacts with free phenol groups produced by enzymatic hydrolysis of the substrate phenyl valerate. Potassium ferricyanide is used as an oxidizing agent. If the enzyme is inhibited by neurotoxic compounds, it cannot carry out the hydrolysis of phenyl valerate, and there are no phenolic groups formed with which AAP can react (colour is less intense). NTE is assayed differentially by addition of paraoxon to the incubation media, resulting in the inhibition of the majority of esterase activity in the tissue homogenate which is not NTE.
Mean NTE activity (± standard deviation), expressed as nmol phenyl valerate/min/g wet brain weight, in the control, test and positive control groups was 2459 ± 109, 2386 ± 79 and 223 ± 90, respectively. Thus, exposure to the test material resulted in a statistically not significant inhibition of ca. 3% in the NTE activity relative to the negative control. NTE activity was statistically significantly reduced by ca. 91% in brain homogenates of the positive control group.
Under the conditions of this study, the test material did not inhibited neurotoxic esterase activity in the hen brain.
Due to unclear composition and impurity profile of the actual test material, the results of this study are doubtful and considered not reliable and insufficient for assessment.
Justification for classification or non-classification
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.