3-diethylaminophenol

Skin irritation / corrosion

Administrative data

Endpoint:

skin irritation: in vivo

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: scientifically acceptable and valid study, limited documentation

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1957

Report date:

1957

Materials and methods

Test guideline

Qualifier:

no guideline followed

Principles of method if other than guideline:

Method: BASF-Test ("Zeittest" 1, 5 , 15 min and 20 h test item exposure on skin and ear)

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

3-diethylaminophenol

EC Number:

202-090-9

EC Name:

3-diethylaminophenol

Cas Number:

91-68-9

Molecular formula:

C10H15NO

IUPAC Name:

3-(diethylamino)phenol

Details on test material:

- Name of test material (as cited in study report): Diäthyl-m-aminophenol
- Physical state: solid
- Analytical purity: 99 %
- Other: soluble in acetic acid, alcohol, lutrol, ethylenechloride, unsoluble in soda solution and vegetable oil

Test animals

Species:

rabbit

Strain:

Vienna White

Test system

Type of coverage:

occlusive

Preparation of test site:

not specified

Vehicle:

other: water or alcohol

Controls:

no

Amount / concentration applied:

TEST MATERIAL
- Amount applied: the application site was covered with powdered and moistened test substance
- Concentration: 50 % in water (pulp) or 50 % in alcohol (almost dissolved)

Duration of treatment / exposure:

- 1, 5, 15 min and 20 h

Observation period:

7- 14 days

Number of animals:

- 2 animals per time point

Details on study design:

Before OECD Guideline 404 was established in 1982, skin irritation was tested using an internal method (BASF test).

White Vienna rabbits were used. Usually, 2 animals were treated for 1, 5 and 15 minutes and 2 other animals for 20 hours using occlusive conditions. An application site of 2.5 X 2.5 cm was covered with the powdered and moistened test substance. In addition, skin tissue from the ear was tested by wrapping the ear.

After the application time of 1, 5 or 15 min, the skin was washed with Lutrol conc and Lutrol 50 % in water.
The skin exposed 20 h to the test substance remained unwashed after application.

Results and discussion

In vivo

Results

Results 1

Irritation parameter:

erythema score

Remarks:

Exposure time 20 hours

Basis:

animal #1

Remarks:

50% in water

Time point:

other: within 14 days

Score:

1

Max. score:

4

Reversibility:

fully reversible within: 48h

Remarks:

slight dequamation on d4

Results 2

Irritation parameter:

edema score

Remarks:

Exposure time 20 hours

Basis:

animal #1

Remarks:

50% in water

Time point:

other: within 14 days

Score:

0

Max. score:

4

Reversibility:

other: not applicable

Results 3

Irritation parameter:

erythema score

Remarks:

Exposure time 20 hours

Basis:

animal #2

Remarks:

50% in water

Time point:

other: within 14 days

Score:

0

Max. score:

4

Reversibility:

other: not applicable

Remarks:

discoloration

Results 4

Irritation parameter:

edema score

Remarks:

Exposure time 20 hours

Basis:

animal #2

Remarks:

50% in water

Time point:

other: within 14 days

Score:

0

Max. score:

4

Reversibility:

other: not applicable

Results 5

Irritation parameter:

erythema score

Remarks:

Exposure time 20 hours

Basis:

animal #1

Remarks:

50% in alcohol

Time point:

other: within 14 days

Score:

0

Max. score:

4

Reversibility:

other: not applicable

Remarks:

discoloration

Results 6

Irritation parameter:

edema score

Remarks:

Exposure time 20 hours

Basis:

animal #1

Remarks:

50% in alcohol

Time point:

other: within 14 days

Score:

0

Max. score:

4

Reversibility:

other: not applicable

Results 7

Irritation parameter:

erythema score

Remarks:

Exposure time 20 hours

Basis:

animal #2

Remarks:

50% in alcohol

Time point:

other: within 14 days

Score:

3

Max. score:

4

Reversibility:

fully reversible within: 9d

Remarks:

slight desquamation on d9

Results 8

Irritation parameter:

edema score

Remarks:

Exposure time 20 hours

Basis:

animal #2

Remarks:

50% in alcohol

Time point:

other: within 14 days

Score:

3

Max. score:

4

Reversibility:

fully reversible within: 9d

Any other information on results incl. tables

The following table shows an overview of the obtained results in the irritation tests.

Erythema and edema were scored immediately after exposure period.

Concentration	Animal	Skin
		1 min	5 min	15 min	20 h
50 % in water	1	Erythema: 1           fully reversible within 24h	Erythema: 1        fully reversible within 24h ;    slight desquamation on d4	Erythema: 1    fully reversible within 24h;       slight desquamation on d4	Erythema: 1     fully reversible within 48h;     slight desquamation on d4
	2	No skin findings	No skin findings	No skin findings	discoloration          No further skin findings
50 % in alcohol	1	Erythema: 2  slight desquamation on d5	Erythema: 2   slight desquamation on d5	Erythema: 2   fully reversible within 5d	discoloration          No further skin findings
	2	Erythema: 1  fully reversible within 5d	Erythema: 1 fully reversible within 5d	Erythema: 2   small patchy necrotic area;  no skin findings on d14	Erythema: 3  Edema:3                fully reversible within 9d;       slight desquamation on d9

Ø no symptom

1 indicated erythema

2 slight erythema

3 well defined erythema

3 well defined edema

4 severe erythema

Findings in ear:

Application for 20 hours in water resulted in no findings.

Application for 20 hours in alcohol resulted in discoloration and erythema (2), beeing reversible within 4 days.