Registration Dossier
Registration Dossier
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EC number: 620-520-7 | CAS number: 194608-78-1
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP-guideline study, validity criteria fulfilled
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 002
- Report date:
- 2002
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: ISO 14593
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- 2-ethylhexylamine
- EC Number:
- 203-233-8
- EC Name:
- 2-ethylhexylamine
- Cas Number:
- 104-75-6
- IUPAC Name:
- 2-ethylhexan-1-amine
- Details on test material:
- - Name of test material (as cited in study report): 2-Ethylhexylamine
- Physical state: liquid
- Analytical purity: 99.9%
- Lot/batch No.: 046340
Constituent 1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): taken from laboratory waste water treatment plants, fed with municipal sewage
- Concentration of sludge: 4 mg/L dry substance
- Preparation of inoculum for exposure: lnoculum was pre-aerated about 24 hours before the start of the test
- Duration of test (contact time):
- 28 d
Initial test substance concentration
- Initial conc.:
- 27.3 mg/L
- Based on:
- test mat.
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- CO2 evolution
- Remarks:
- %TIC/ThIC
- Details on study design:
- TEST CONDITIONS
- Stock solution:
151,9 mg test substance dissolved in 5000 ml mineral medium
Before COD analysis the stock solution with test substance in mineral medium was stirred for about 20 hours. The pH value was adjusted to 7.4 with 1 mol. sulfuric acid.
Test medium:
27.3 mg/l test substance = 90 ml/vessel stock solution
0.5 ml/vessel inoculum (800 mg/L dry medium per vessel)
9.5 ml/vessel water
Total volume: 100 ml
- Test temperature: 22 ± 2 °C
- pH: 6.7
CONTROL AND BLANK SYSTEM
- Inoculum blank: 1
- Abiotic sterile control: 1
- Toxicity control: 1
- Reference control: 1
Reference substance
- Reference substance:
- aniline
- Remarks:
- TOC: 396 mg/L
Results and discussion
% Degradation
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 70 - 80
- Sampling time:
- 28 d
- Details on results:
- Kinetic of test substance (in %):
8 after 3 days
33 after 7 days
60 after 10 days
72 after 28 days
BOD5 / COD results
- Results with reference substance:
- Kinetic of control substance (in %):
80 - 90 after 14 days
Applicant's summary and conclusion
- Interpretation of results:
- readily biodegradable
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