Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 205-861-8 | CAS number: 156-62-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: dermal
Administrative data
- Endpoint:
- acute toxicity: dermal
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1989-05-10 to 1989-05-14
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 989
- Report date:
- 1989
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 402 (Acute Dermal Toxicity)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method B.3 (Acute Toxicity (Dermal))
- Version / remarks:
- (Annex V of the 6th amendment of the EEC directive of 18th September, 1979, Part B, 4.1.3 Acute toxicity dermal)
- Deviations:
- no
- GLP compliance:
- yes
- Test type:
- standard acute method
- Limit test:
- yes
Test material
- Reference substance name:
- Calcium cyanamide
- EC Number:
- 205-861-8
- EC Name:
- Calcium cyanamide
- Cas Number:
- 156-62-7
- Molecular formula:
- CN2.Ca
- IUPAC Name:
- calcium cyanoazanediide
- Reference substance name:
- Calcium oxide
- EC Number:
- 215-138-9
- EC Name:
- Calcium oxide
- Cas Number:
- 1305-78-8
- Molecular formula:
- CaO
- IUPAC Name:
- oxocalcium
- Reference substance name:
- Carbon
- EC Number:
- 231-153-3
- EC Name:
- Carbon
- Cas Number:
- 7440-44-0
- Molecular formula:
- C
- IUPAC Name:
- carbon
- Reference substance name:
- Hematite (Fe2O3)
- EC Number:
- 215-275-4
- EC Name:
- Hematite (Fe2O3)
- Cas Number:
- 1317-60-8
- Molecular formula:
- Fe2O3
- IUPAC Name:
- diiron oxide
- Reference substance name:
- Urea
- EC Number:
- 200-315-5
- EC Name:
- Urea
- Cas Number:
- 57-13-6
- Molecular formula:
- CH4N2O
- IUPAC Name:
- urea
- Reference substance name:
- Silicon dioxide
- EC Number:
- 231-545-4
- EC Name:
- Silicon dioxide
- Cas Number:
- 7631-86-9
- Molecular formula:
- O2Si
- IUPAC Name:
- dioxosilane
- Reference substance name:
- Trisilicon tetranitride
- EC Number:
- 234-796-8
- EC Name:
- Trisilicon tetranitride
- Cas Number:
- 12033-89-5
- Molecular formula:
- N4Si3
- IUPAC Name:
- trisilicon tetranitride
- Reference substance name:
- Calcium dihydroxide
- EC Number:
- 215-137-3
- EC Name:
- Calcium dihydroxide
- Cas Number:
- 1305-62-0
- Molecular formula:
- CaH2O2
- IUPAC Name:
- calcium dihydroxide
- Reference substance name:
- Aluminium oxide
- EC Number:
- 215-691-6
- EC Name:
- Aluminium oxide
- Cas Number:
- 1344-28-1
- Molecular formula:
- Al2O3
- IUPAC Name:
- aluminium oxide
- Reference substance name:
- Cyanoguanidine
- EC Number:
- 207-312-8
- EC Name:
- Cyanoguanidine
- Cas Number:
- 461-58-5
- Molecular formula:
- C2H4N4
- IUPAC Name:
- 2-cyanoguanidine
- Reference substance name:
- Calcium acetylide
- EC Number:
- 200-848-3
- EC Name:
- Calcium acetylide
- Cas Number:
- 75-20-7
- Molecular formula:
- C2Ca
- IUPAC Name:
- calcium ethynediide
- Reference substance name:
- unknown
- IUPAC Name:
- unknown
- Test material form:
- solid: particulate/powder
- Details on test material:
- Kalkstickstoff (calcium cyanamide, technical grade)
Constituent 1
impurity 1
impurity 2
impurity 3
impurity 4
impurity 5
impurity 6
impurity 7
impurity 8
impurity 9
impurity 10
impurity 11
Test animals
- Species:
- rabbit
- Strain:
- New Zealand White
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: A. Smith, Warlingham, Surrey, England
- Age at study initiation: approximately 9 - 12 weeks
- Weight at study initiation: weight range of 2.7 - 3.1 kg
- Fasting period before study: no
- Housing: individually in metal cages with perforated floors
- Diet: standard laboratory diet ad libitum
- Water: ad libitum
- Acclimation period: 14 days prior to start of the main study
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 20 - 21°C
- Humidity (%): 54%
- Air changes (per hr): 15 air changes/hour
- Photoperiod (hrs dark / hrs light): 12 hours artificial light in each 24 hour period (i.e. 12 hours dark / 12 hours light)
Administration / exposure
- Type of coverage:
- occlusive
- Vehicle:
- water
- Details on dermal exposure:
- TEST SITE
- Area of exposure: dorso-lumbar region
- % coverage: 10% of total body surface
- Type of wrap if used: the treated area was covered with gauze, held in place with an impermeable dressing encircled firmly around the trunk
REMOVAL OF TEST SUBSTANCE
- Washing: washing with warm (30-40°C) water and blotting dry with absorbent paper
- Time after start of exposure: 24 hours
TEST MATERIAL
- Amount(s) applied (volume or weight with unit): 2 g/kg bw, both for preliminary and main study
- Concentration (if solution): test substance moistened with distilled water at 1 mL/kg
- Constant volume or concentration used: yes
- For solids, paste formed: yes, test substance moistened with distilled water at 1 mL/kg
VEHICLE
- Amount(s) applied (volume or weight with unit): test substance moistened with distilled water at 1 mL/kg - Duration of exposure:
- 24 hours
- Doses:
- 2 g/kg bw
- No. of animals per sex per dose:
- preliminary study: one male and one female
main study: 5 male and 5 female - Control animals:
- no
- Details on study design:
- - Duration of observation period following administration: Animals of the preliminary and main studies were observed for 5 and 14 days, respectively, after dosing.
- Frequency of observations and weighing: Animals were observed soon after dosing, then at frequent intervals for the remainder of Day 1 and at subsequent days at least twice per day. Individual bodyweights of rabbits in the main study were recorded on Days 1, 8 and 15.
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs were recorded at each observation (nature, severity, approximate time of onset and duration of toxic sign), body weight, macroscopic post mortem examination (opening the abdominal and thoracic cavities) - Statistics:
- No data
Results and discussion
- Preliminary study:
- The results of the preliminary study performed on two rabbits (one male and one female) indicated that the acute median lethal dermal dose to male and female rabbits of calcium cyanamide, technical grade, was greater than 2 g/kg bw.
Effect levels
- Key result
- Sex:
- male/female
- Dose descriptor:
- LD0
- Effect level:
- > 2 000 mg/kg bw
- Remarks on result:
- other: no mortality occurred
- Mortality:
- There were no deaths following a single dermal dose of calcium cyanamide, technical grade, at 2 g/kg bw.
- Clinical signs:
- other: There were no clinical signs of systemic toxicity in response to treatment.
- Gross pathology:
- At terminal autopsy, red streaks were observed in the left ventricle of the heart of one male rabbit. Small cysts were also observed on the outer surface of the kidney of the same male. A large fluid-filled cyst (10 x 10 cm) was also present in the kidney. Small cysts were also observed on the surface of the kidneys of one other male.
- Other findings:
- Dermal responses:
Well-defined to severe erythema accompanied by well-defined to moderate erythema were observed in all treated animals after removal of the dressings. Irritation had resolved in two male and two female rabbits by Day 10 or 11 and in further two animals (one male and one female) by Day 14. Irritation was still present in the remaining two males and two females at the end of the study (Day 15).
Small areas of necrosis were present in one male and two females from Day 2 until the end of the study. Necrotic patches were observed in a further three males and two females from Day 3 and until Day 9 (with the exception of one male where the necrotic patch was still present at the end of the study).
Any other information on results incl. tables
no remarks
Applicant's summary and conclusion
- Interpretation of results:
- GHS criteria not met
- Conclusions:
- The acute lethal dose to rabbits of calcium cyanamide, technical grade, was found to be > 2 g/kg bw.
- Executive summary:
The study was designed to assess the acute dermal toxicity of calcium cyanamide, technical grade, following a single dose onto rabbit skin. The test substance was applied as supplied, moistened sufficiently with distilled water to ensure good contact with the skin.
A preliminary study (trial test) was performed in one male and one female rabbit using 2 g/kg bw of test substance. Animals were observed for 5 days after dosing and results indicated that the acute median lethal dose of the test substance was > 2g /kg bw.
For the main study, a group of ten rabbits (5 male and 5 female) were treated at 2 g/kg bw of calcium cyanamide.
One day prior to treatment, hair was removed from the dorso-lumbar region with electric clippers exposing an area equivalent to 10% of the total body surface. No shaving or chemical depilation was used. The test substance was spread evenly over the prepared skin and was then covered with gauze which was held in place with impermeable dressing encircled firmly around the trunk.
At the end of the 24 hour exposure period, the dressings were carefully removed and the treated area decontaminated by washing with warm water and blotting with absorbent paper.
Animals were observed for skin irritation and clinical signs for 14 days after dosing.
All animals of the main study were killed on Day 15 by an intravenous overdose of phenobarbitone sodium and were subjected to macroscopic post mortem examination.
There were no death and no clinical signs of systemic toxicity observed throughout the study period. Erythema and edema as well as necrotic patches were observed in all of the animals with signs of irritation being present at the end of the observation period in three of the ten animals tested.
A slightly lower bodyweight gain was recorded for one female on Day 15 and two males showed cysts on the surface of the kidney at terminal autopsy. All other animals were without findings regarding bodyweight and post mortem examination.
The acute lethal dose to rabbits of calcium cyanamide, technical grade, was found to be > 2 g/kg bw for both males and females.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.