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EC number: 213-554-5 | CAS number: 976-71-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- Oct 2000 - Jan 2003
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: According to OECD guideline under GLP
Cross-referenceopen allclose all
- Reason / purpose for cross-reference:
- reference to same study
- Reason / purpose for cross-reference:
- reference to other study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 004
- Report date:
- 2004
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Deviations:
- yes
- Principles of method if other than guideline:
- EC guideline Part 2 - Testing Methods, Part C.1
- GLP compliance:
- yes
Test material
- Reference substance name:
- Canrenone
- EC Number:
- 213-554-5
- EC Name:
- Canrenone
- Cas Number:
- 976-71-6
- Molecular formula:
- C22H28O3
- IUPAC Name:
- 10,13-dimethyl-1,8,9,10,11,12,13,14,15,16-decahydro-3'H-spiro[cyclopenta[a]phenanthrene-17,2'-furan]-3,5'(2H,4'H)-dione
- Details on test material:
- - Name of test material (as cited in study report): Canrenone
- Analytical purity: 98.4%
- Lot/batch No.: 90515001
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
Test solutions
- Vehicle:
- no
Test organisms
- Test organisms (species):
- Pimephales promelas
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 96 h
Results and discussion
Effect concentrations
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 7.7 - 15.2 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
Any other information on results incl. tables
- Sublethal observations / clinical signs:
Table 1: Mortality of fish exposed to canrenone over 96 hours
Dilution of canrenone concentration [mg/L] 3 hours 6 hours 24 hours 48 hours 72 hours 96 hours 0 control (tap water) 0 0 0 0 0 0 0 1:80 0,1 0 0 0 0 0 0 1:40 0,3 0 0 0 0 0 0 1:20 0,8 0 0 0 0 0 0 1:10 1,5 0 0 0 0 0 0 1:5 3,2 0 0 0 0 0 0 1:2,5 7,7 0 0 1) 1) 1) 1) Saturated solution (limit test) 15,2 (TOC analysis) 10 (after 20 min) - - - - - 1) all fish (10 of 10) sacrified in moribund status
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The LC50/96 hours of canrenone can be expected between 7.7 and 15.2 mg/L taking into account
that all fish died in a saturated solution (which was measured during the limit test to contain
15.2 mg/L). Although mortality is only expected at the saturated solution, the results of this
study showed that slight effects occurred at concentrations from 1.5 mg/L upwards and severe
effects at concentrations from 3.2 mg/L upwards.
Therefore, canrenone is toxic to fish, since effects were observed below 10 mg/L. - Executive summary:
The purpose of this study was to detennine the acute toxicity including the LCso/96 hours of canrenone (ZK 57587) to the fathead minnow Pimephales prome1as. Canrenone is an intermediate of the synthesis of Eplerenone, a carbolactone derivative. The study was conducted in accordance with the test guideline OECD 203, and the EC guideline Part 2 - Testing Methods, Part C.1. In a preceeding limit test, which is not reported in detail, all fish died in an saturated solution.
Ten fish (fathead minnow Pimephales promelas) were used for each test solution of canrenone and ten for the control group. The fish were exposed for a period of 96 hours under static conditions. Mortality and visible abnonnalities were recorded at approximately 3, 6, 24, 48, 72 and 96 hours. For the preparation ofthe test solution a suspension (1 liter) with a nominal Ioading of 800 mg/L canrenone was ultrasonified for 30 minutes. Thereafter the suspension was filied up to 8 liters and stirred for approximately 24 hours. This suspension was filtered through a glassfibre filter. After filtration, the resulting solution served as stock solution. It was diluted 1:2.5,1:5, 1:10, 1 :20, 1 :40, and 1 :80 to obtain the final test solutions.
The concentration ofthe test solutions were analyzed by HPLC at 0,48 and 96 hours. The concentration of canrenone in the stock solution was determined by TOC only. It was 15.2 g/L.
No mortality but abnormal behavior was observed in the dilutions 1 :2.5, 1:5 and 1: 10 of canrenone. Fish at the highest concentration had to be killed after 24 hours in a moribund status for humane reasons.
The observations showed that, although not mortality occurred up to a eoneentration of 7.7 mgIL (1:2.5 dilution), slight effeets were seen at 1.5 mg/L (1: 10 dilution) and severe effects from 3.2 mg/L (1:5 dilution) upwards. The highest concentration without effects was 0.8 mg/L (dilution 1 :20).
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