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EC number: 235-468-7 | CAS number: 12237-62-6 This substance is identified in the Colour Index by Colour Index Constitution Number, C.I. 42535:3.
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: oral
Administrative data
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- April 1974
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Justification for type of information:
- Data is from study report.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 974
- Report date:
- 1974
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: As mentioned below
- Principles of method if other than guideline:
- Acute oral toxicity of test chemical in rats.
- GLP compliance:
- yes
- Test type:
- acute toxic class method
- Limit test:
- no
Test material
- Reference substance name:
- Ferrate(4-), hexakis(cyano-C)-, methylated 4-[(4-aminophenyl)(4-imino-2,5-cyclohexadien-1-ylidene)methyl]benzenamine copper(2+) salts
- EC Number:
- 235-468-7
- EC Name:
- Ferrate(4-), hexakis(cyano-C)-, methylated 4-[(4-aminophenyl)(4-imino-2,5-cyclohexadien-1-ylidene)methyl]benzenamine copper(2+) salts
- Cas Number:
- 12237-62-6
- Molecular formula:
- [C24N3H28] Cu2Fe(CN)6
- IUPAC Name:
- ferrate(4-), hexakis(cyano-C)-, methylated 4-[(4-aminophenyl)(4-imino-2,5-cyclohexadien-1-ylidene)methyl]benzenamine copper(2+) salts
- Test material form:
- solid
- Details on test material:
- IUPAC name: ferrate(4-), hexakis(cyano-C)-, methylated 4-[(4-aminophenyl)(4-imino-2,5-cyclohexadien-1-ylidene)methyl]benzenamine copper(2+) salts
Substance type: Organic
Physical state: Solid
Molecular Formula: [C24N3H28] Cu2Fe(CN)6
Molecular Weight: 697.54 g/mol
Constituent 1
Test animals
- Species:
- rat
- Strain:
- other: Tif. RAI
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Age at study initiation: 6 to 7 weeks old
- Weight at study initiation: 160 to 180 g.
- Fasting period before study: The rats were starved during one night before starting the treatment.
- Housing: The males and females were segregated and housed in Macrolon cages. (Type 3) in groups of 5 in a room.
- Diet (e.g. ad libitum): They received water and food (NAFAG, Gossau SG, rat food) ad libitum.
- Breeding : The compound was tested on 50 Tif. RAI rats (25 males/25 females), bred under SPF conditions in our own breeding unit.
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 22±1 °C
- Humidity (%): a relative humidity of approximately 50 %
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- CMC (carboxymethyl cellulose)
- Remarks:
- 2 %
- Details on oral exposure:
- DOSAGE PREPARATION:
Test chemical was weighed into an Erlenmeyer flask on a Mettler balance. It was suspended at 10 and 30 % with carboxymethylcellulose 2 % and administered by oral intubation. Before treatment the suspension was homogeneously dispersed with an Ultra-Turrax and during treatment it was kept stable with a magnetic stirrer. - Doses:
- Doses: 1000; 3170; 3590; 4640 and 6000 mg/kg
- No. of animals per sex per dose:
- 1000 mg/kg: 5 males and 5 females
3170 mg/kg: 5 males and 5 females
3590 mg/kg: 5 males and 5 females
4640 mg/kg: 5 males and 5 females
6000 mg/kg: 5 males and 5 females - Control animals:
- no
- Details on study design:
- No data
- Statistics:
- No data
Results and discussion
- Preliminary study:
- No data
Effect levels
- Sex:
- male/female
- Dose descriptor:
- LD50
- Effect level:
- > 6 000 mg/kg bw
- Based on:
- test mat.
- Remarks on result:
- other: No mortality was observed
- Mortality:
- No mortality was observed.
- Clinical signs:
- other: Symptoms: Within 2 hours after treatment the rats in all dosage groups showed sedation, dyspnoea, exophthalmus, curved position and ruffled fur. These symptoms became more accentuated as the dose was increased.
- Gross pathology:
- Autopsy: Dead and killed animals: No substance related gross organ changes were seen.
- Other findings:
- The surviving animals had recovered within 6 to 7 days. They were killed and autopsied after an observation period of 7 days.
Any other information on results incl. tables
Table:
Dose mg/kg |
Concentration % of formulation |
No. of animals |
Died within |
||||||||
2 hrs. |
24 hrs. |
48 hrs. |
7 days |
||||||||
Male |
Female |
Male |
Female |
Male |
Female |
Male |
Female |
Male |
Female |
||
1000 |
10 |
5 |
5 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
3170 |
30 |
5 |
5 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
3590 |
30 |
5 |
5 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
4640 |
30 |
5 |
5 |
0 |
0 |
0 |
0 |
0 |
0 |
1 |
0 |
6000 |
30 |
5 |
5 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
3 |
No higher doses were possible.
Applicant's summary and conclusion
- Interpretation of results:
- other: Not classified
- Conclusions:
- The acute oral LD50 of test chemical in rats of both sexes observed over a period of 7 days is >6000 mg/kg.
- Executive summary:
The acute oral toxicity study was conducted by using test chemical in groups of 10 male and female Tif. RAI rats at the dose concentration of 1000; 3170; 3590; 4640 and 6000 mg/kg bw dissolved in CMC (carboxymethyl cellulose). Test chemical was weighed into an Erlenmeyer flask on a Mettler balance. It was suspended at 10 and 30 % with carboxymethylcellulose 2 % and administered by oral intubation. Before treatment the suspension was homogeneously dispersed with an Ultra-Turrax and during treatment it was kept stable with a magnetic stirrer. Animals were observed for mortality, clinical signs and gross necropsy. No mortality was observed. Within 2 hours after treatment the rats in all dosage groups showed sedation, dyspnoea, exophthalmus, curved position and ruffled fur. These symptoms became more accentuated as the dose was increased. The surviving animals had recovered within 6 to 7 days. They were killed and autopsied after an observation period of 7 days. No substance related gross organ changes were seen in dead and killed animals. Hence, LD50 value was considered to be >6000mg/kg bw, when groups of 10 male and female Tif. RAI rats were treated with Pigment Violet 27 vai oral gavage route. Thus the substance is considered to be not classified in the toxic category.
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