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Diss Factsheets
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EC number: 428-100-3 | CAS number: 94239-04-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Deviations:
- yes
- Remarks:
- The fish were held for 6 days at approximately 15°C before the test. This is less than the guideline requirement of being on site for 12 days and acclimatised for at least 7 days.
- GLP compliance:
- yes
- Specific details on test material used for the study:
- Substance ID: F6TF
Lot #: AF0460
Purity: 99.5% - Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: all
- Sampling method: high performance liquid chromatography; Samples were taken from the centre of the test solution. - Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances): The test solution was prepared by the direct addition of the required amount of the test substance to the exposure vessel. The test solution was then stirred thoroughly for 24 hours to allow complete dissolution of the test substance. The test solutions were observed to be clear and colourless. The test solution was not changed or aerated during the 96 hour exposure period.
- Test organisms (species):
- Oncorhynchus mykiss (previous name: Salmo gairdneri)
- Details on test organisms:
- TEST ORGANISM
- Common name: Rainbow trout
- Source: Houghton Springs Fish Farm, Winterboume Houghton, Blandford Forum, Dorset, UK
- Length at study end: 35 - 41 mm; mean 38 mm
- Weight at study end: 0.54 - 1.24 g; mean 0.76 g
- Feeding during test: no
ACCLIMATION
- Acclimation period: 6 days
- Acclimation conditions (same as test or not): yes; 15°C
- Type and amount of food: Keystart 1200® (FF417)
- Health during acclimation (any mortality observed): 1 dead - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Hardness:
- 42.3 mg/L as CaC03
- Test temperature:
- 15 ± 1°C
- pH:
- 6.98 - 7.89
- Dissolved oxygen:
- 3.6 - 10.0 mg/L
- Salinity:
- freshwater
- Nominal and measured concentrations:
- Nominal: 0, 10, 18, 32, 56 and 120 mg/L
Measured: 0, 11, 19, 34, 58 and 110 mg/L - Details on test conditions:
- TEST SYSTEM
- Test vessel: spherical glass vessels of 36 cm external diameter, with a nominal capacity of 20 L, fitted with Quickfit glass® lids and stoppers (sealable due to volatile test substance)
- Renewal rate of test solution (frequency/flow rate): none
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
EFFECT PARAMETERS MEASURED (with observation intervals if applicable): Observations for mortalities and symptoms of toxicity were made at 24, 48, 72 and 96 hours.
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 82 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: 56 - 120 mg/L
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 32 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: symptoms of toxicity
- Details on results:
- The mean measured values of the test solutions ranged from 100 to 106% of the nominal values.
Dissolved oxygen values ranged from 3.6 to 10.0 mg/L. No mortalities occurred in the low DO test vessels.
The fish in the 120 mg/L test solution were terminated at 3.75 hours. Their symptoms were loss of balance, dark discoloration and laboured breathing. - Reported statistics and error estimates:
- The LC50 values, and their respective 95% confidence intervals, were calculated at various time intervals by the testing facility computer program "LC50" using Stephan's method
- Sublethal observations / clinical signs:
Observed Symptoms of Toxicity
Nominal Concentration (mg/L)
24 hour
48 hour
72 hour
96 hour
10
A
A
A
A
18
A
A
A
A
32
A
A
A
A
56
Cabc
Cabc
Cabc
Cc
120
TD*
TD
TD
TD
A = 10% or less of test population exhibiting symptoms of toxicity (considered not to be biologically significant).
B = Between 11 % and 30% of test population exhibiting symptoms of toxicity.
C = More than 30% of test population exhibiting symptoms of toxicity.
TD* = Terminated after 3.75 hours on day 0
a = Quiescent
b = Surfacing
c = Dark discoloured
- Validity criteria fulfilled:
- yes
- Conclusions:
- 96-h LC50 (Rainbow Trout) = 82 mg/L
- Executive summary:
An acute static 96-hour test was conducted at concentrations of 0, 10, 18, 32, 56 and 120 mg/L on rainbow trout according to the guideline OECD 203. The test was run in sealed vessels because of the volatility of the test substance. After 3.75 hours the test at 120 mg/L was terminated due to the toxic effects on the fish. Based on nominal concentration, the 96-hour LC50 was 82 mg/L.
Reference
Description of key information
Based on nominal concentrations because mean measured concentrations were within 80-120% of nominal:
96-hour LC50 = 82 mg/L
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 82 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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