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EC number: 205-187-4 | CAS number: 135-37-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: dermal
Administrative data
- Endpoint:
- acute toxicity: dermal
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- From Oct. 1985 to Nov. 1985
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Limitations in study performance and reporting
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 985
- Report date:
- 1985
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 402 (Acute Dermal Toxicity)
- Deviations:
- yes
- Remarks:
- See below
- Principles of method if other than guideline:
- Deviations: One single dose was tested, however this dose was 2 mL/kg of a 40% slurry and not 2000 mg/kg bw; 3 animals (rabbits)/sex were used; 3 animals out of 6 animals were abraded while guideline suggests the use of intact animals
- GLP compliance:
- no
- Test type:
- standard acute method
- Limit test:
- no
Test material
- Reference substance name:
- Disodium 2-hydroxyethyliminodi(acetate)
- EC Number:
- 205-187-4
- EC Name:
- Disodium 2-hydroxyethyliminodi(acetate)
- Cas Number:
- 135-37-5
- Molecular formula:
- C6H9NO5.Na2
- IUPAC Name:
- disodium 2-hydroxyethyliminodi(acetate)
- Reference substance name:
- N-(2-Disodium 2-hydroxyethyliminodi(acetate)
- IUPAC Name:
- N-(2-Disodium 2-hydroxyethyliminodi(acetate)
- Test material form:
- solid: particulate/powder
- Remarks:
- migrated information: powder
- Details on test material:
- - Name of test material: Hydroxyethyliminodiacetic acid Na salt (HEIDA, Na2)
- TSIN: E2748.01
- Molecular formula: Not reported
- Molecular weight: Not reported
- Substance type: Pure active substance
- Physical state: White powder
- Stability under test conditions: Not reported
- Storage condition of test material: Room temperature
Constituent 1
Constituent 2
Test animals
- Species:
- rabbit
- Strain:
- New Zealand White
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Ranch Rabbits, Crawley
- Age at study initiation: 11 to 20 weeks
- Weight at study initiation: 2.74 to 3.22 kg
- Fasting period before study: Not reported
- Housing: Animals were individually housed
- Diet : SOC Rabbit Diet, Special Diets Services Ltd, Stepfield, ad libitum
- Water: Ad libitum. Water was supplied by an automatic system. The diet and drinking water were considered not to contain any contaminant at a level that might have affected the objectives or integrity of the study. Water bottles were cleaned at weekly intervals.
- Acclimation period: At least 7 days
ENVIRONMENTAL CONDITIONS
- Temperature: 16 to 20 °C
- Humidity: Not reported
- Air changes (per hr): Not reported
- Photoperiod: A cycle of 14 hours fluorescent light (6.00-20.00 h) and 10 hours darkness
IN-LIFE DATES: Not reported
Administration / exposure
- Type of coverage:
- occlusive
- Vehicle:
- water
- Remarks:
- Distilled
- Details on dermal exposure:
- APPLICATION OF TEST MATERIAL:
- The day before treatment the back and flank of each animal were clipped free of hair using electric clippers.
- The skin of 3 animals (2 males, 1 female) were left intact and the skin of the other 3 animals (1 male, 2 females) was abraded with the clipper head so as to penetrate the horny layer of the epidermis but without causing bleeding.
- Type of wrap used: The test material was applied uniformly to the shaved area and a porous gauze dressing was placed over the treated area and the treatment area occluded with a strip of impermeable plaster wound around the trunk of the animal.
- Each animal was placed in a plastic collar for the treatment period to prevent removal of the wrappings.
- After a 24 hour contact period the wrappings were cut through using blunt ended scissors and removed.
TEST SITE
- Area of exposure: Back and flanks of animal
- % coverage: Not reported
REMOVAL OF TEST SUBSTANCE
- Washing: Yes. Treated skin and surrounding area were wiped with a moist disposable towel
- Time after start of exposure: 24 hours
TEST MATERIAL
- Amount(s) applied: 2 mL/kg
- Constant volume or concentration used: Yes
- For solids, paste formed: Yes. The test material was formulated as 40% w/v slurry in distilled water. The pH was adjusted with sodium hydroxide to 10.5 - Duration of exposure:
- 24 hours
- Doses:
- 2 mL/kg
- No. of animals per sex per dose:
- 3 animals per sex
- Control animals:
- not required
- Details on study design:
- - Duration of observation period following administration: 14 days
- Frequency of observations and weighing: Animals were observed daily for 14 days. Body weights were recorded on the day of treatment and on Day 14 of observation.
- Necropsy of survivors performed: Yes
- Other examinations performed: Clinical signs, body weight, skin reaction, and necropsy.
Results and discussion
Effect levels
- Sex:
- male/female
- Dose descriptor:
- LD50
- Effect level:
- > 2 mL/kg bw
- Based on:
- test mat.
- Remarks on result:
- other: The test material was administered as 40% w/v slurry.
- Mortality:
- Two animals with abraded skin died within 96 hours of treatment. These differences were too small to positively infer a difference between intact and abraded animals.
- Clinical signs:
- other: Immediately prior to death lethargy, prostration and rapid respiration were observed in one animal. Slight erythema was noted in all animals with intact skin up to 3 days after treatment and in the 2 animals with abraded skin. Slight to moderate atonia wa
- Gross pathology:
- All animals were necropsied. No abnormalities were detected in those animals killed at termination. Gelatinous fluid in the abdominal cavity was observed in animals that died during the study. Lung discolouration was also observed which may have been an agonal change.
Applicant's summary and conclusion
- Interpretation of results:
- GHS criteria not met
- Conclusions:
- The acute percutaneous toxicity (LD50) of Hydroxyethyliminodiacetic acid Na salt was >2 mL/kg bw when administered as a 40% w/v slurry to New Zealand White rabbits.
- Executive summary:
The acute dermal toxicity of hydroxyethyliminodiacetic acid Na salt was performed by following methods similar to the OECD Guideline 402 (Acute Dermal Toxicity).
Six young adult New Zealand white rabbits (3 males and 3 females) weighing 2.74 to 3.22 kg obtained from Ranch Rabbits, Crawley were used in the study. The animals were individually housed in a single air-conditioned room routinely maintained at temperature 16-20 °C. The animals were allowed free access to water and food (SQC Rabbit Diet, Special Diets Services Ltd., Stepfield). Animals were acclimatized for at least 7 days prior to the initiation of study.
One day before treatment, the back and flank of each animal were clipped free of hair using electric clippers. The test material was formulated as 40% w/v slurry in distilled water and applied at a dose level of 2 mL/kg. The test material was applied uniformly to the shaved area and a porous gauze dressing was placed over the treated area. The treatment area was occluded with a strip of impermeable plaster wound around the trunk of the animal. Each animal was placed in a plastic collar for the treatment period to prevent removal of the wrappings.
After a 24 hour contact period, the wrappings were removed. The treated skin and surrounding areas were wiped with a moist disposable towel to remove any residual test material.
The test sites were examined for local skin reactions. Observations for mortality, local skin reactions and behavioral abnormality were continued for a total of 14 days following the skin applications. Individual body weights were recorded on the day of treatment and on Day 14 of observation. A necropsy examination was conducted in all animals.
2 of the 3 animals with abraded skin died within 96 hours of treatment. All surviving animals showed normal body weight gains at termination.
The conclusion that mortality was treatment related was considered equivocal in view of the absence of changes in the surviving animals. The possibility exist that the deaths were caused by an exacerbation of an underlying pathological condition. This suggestion was supported by the presence of gelatinous fluid at necropsy in the animals that died during the study, suggesting the possibility of an infection.
Based on above it was concluded that, the acute percutaneous toxicity (LD50) of Hydroxyethyliminodiacetic acid Na salt was > 2 mL/kg bw when administered as 40% w/v slurry to New Zealand White rabbits.
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