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Diss Factsheets
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EC number: 253-775-4 | CAS number: 38083-17-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic plants other than algae
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic plants other than algae
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 221 (Lemna sp. Growth Inhibition Test)
- GLP compliance:
- no
- Remarks:
- published data
- Analytical monitoring:
- yes
- Details on sampling:
- Samples for chemical analysis were taken at the start of the tests and at the end from control, lowest and highest test concentrations.
- Vehicle:
- no
- Details on test solutions:
- Stock solutions were prepared by ultrasound treatment for 15 minutes and stirring over night in the dark at room temperature.
Test solutions were prepared as dilutions of the stock solution directly before test start. - Test organisms (species):
- Lemna minor
- Details on test organisms:
- TEST ORGANISM
- Common name: Lemna minor
- Strain: clone St, Landlot number 9441
- Source (laboratory, culture collection): in-house batch culture, from Friedrich-Schiller University Jena, K.-J. Appenroth
- Method of cultivation: Steinberg medium modified acc. R. Altenburger (annex OECD 221) - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 7 d
- Test temperature:
- 24 +/- 2°C
- pH:
- 5.6/6.9; 6.2
- Nominal and measured concentrations:
- Nominal concentrations: 0.008 - 0.2 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: crystallizing dishes
- Material, size, headspace, fill volume: Fill volume 200 mL
- Control end cells density: no data
- No. of colonies per vessel: 4
- No. of fronds per colony: 3
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 6
GROWTH MEDIUM
- Standard medium used: yes, Steinberg medium modified acc. R. ALtenburger (annex OECD 221)
OTHER TEST CONDITIONS
- Photoperiod: continuous
- Light intensity and quality: photosynthetically active radiation (85 - 135 µE/m²xs) - Key result
- Duration:
- 7 d
- Dose descriptor:
- EC50
- Effect conc.:
- 0.024 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- frond number
- growth rate
- Key result
- Duration:
- 7 d
- Dose descriptor:
- EC10
- Effect conc.:
- 0.008 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- frond number
- growth rate
- Duration:
- 7 d
- Dose descriptor:
- EC50
- Effect conc.:
- 0.013 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Duration:
- 7 d
- Dose descriptor:
- EC10
- Effect conc.:
- 0.004 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Reported statistics and error estimates:
- Results of the standard biotests were modeled as concentration–response curves in the free software R version 2.11.1 using the drc package. Effects (as percentage of control for continuous data and as proportion for binomial data) were fitted against log-transformed concentrations (x) using the 4-parameter log–logistic model (function LL.4) given in Equation 1 in publication, with the parameter c relating to the lower and d relating to the upper limit of the curve and b relating to the slope. The EC50 was directly iteratively determined as the model parameter. Whereas for the continuous response data obtained for L.
minor, the 4-parameter model was used. Further effect concentrations, EC20 and EC10, were estimated by the ED function in drc and confidence intervals (95%) for all point estimates were determined using the delta method in drc.
Concentration–response modeling was based on nominal test concentrations. - Validity criteria fulfilled:
- yes
- Remarks:
- frond number doubling time < 2.5 days: OK (1.8)
- Conclusions:
- The 7-d ErC50 and 7-d ErC10 based on the growth rate and frond number of the test item to Lemna minor were determined to be 0.0239 and 0.0079 mg/L, respectively.
- Executive summary:
A published study on the biological effect of the substance to the aquatic plant Lemna minor is available. mg/l climbazole (98% purity) using Steinberg medium. Based on preliminary range-finding test, the nominal exposure concentrations in the definitive test were 0.008, 0.0031, 0.0125, 0.05 and 0.2 mg/L. Test solutions were prepared by ultrasonic mixing for 15 minutes and continuous stirring overnight in the dark. A static regime was employed with 3 replicates per test concentration and 6 control replicates. Static test conditions were within protocol guidelines. The test media pH was 5.6-6.2. Study observations were recorded and noted smaller frond of darker colouration and clustered colonies at higher test concentrations. The concentrations were determined by High Performance Liquid Chromatography (HPLC) and resulted in a recovery of 70.5% of the nominal concentrations. The 7-d ErC50 and 7-d ErC10 based on the growth rate and frond number of the test item to Lemna minor were determined to be 0.0239 and 0.0079 mg/L, respectively. The ErC10 of this test is considered for the hazard assessment and classification.
Reference
Chemical analysis of the test solutions confirmed that recovery of nominal climbazole concentrations was moderate for all aquatic biotests mentioned in the publication, at 68% to 85% for initial and 62% to 74% for aged test solutions. However, based on initially
measured test concentrations, recovery at test end reached 81% to 99%. Hence, there was only a little loss of climbazole during
the static exposure.
The mean recovery the substance in the this test was 70.5% (information from the author). Thus, the ErC10 (growth rate) based on the measured concentrations were used for the hazard assessment.
Description of key information
The 7-d ErC50 and 7-d ErC10 based on the growth rate and frond number of the test item to Lemna minor were determined to be 0.0239 and 0.0079 mg/L, respectively. The ErC10 of this test is considered for the hazard assessment and classification.
Key value for chemical safety assessment
- EC50 for freshwater plants:
- 0.024 mg/L
- EC10 or NOEC for freshwater plants:
- 0.008 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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