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EC number: 254-837-3 | CAS number: 40203-73-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: oral
Administrative data
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1977
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Pre-GLP study following a method similar a recognised guideline.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 977
- Report date:
- 1977
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 401 (Acute Oral Toxicity)
- Deviations:
- no
- Principles of method if other than guideline:
- The principles of the method were in accordance with the US 16 CFR 1500.3 definitions.
- GLP compliance:
- no
- Test type:
- standard acute method
- Limit test:
- no
Test material
- Reference substance name:
- Methyl cyclopentylideneacetate
- EC Number:
- 254-837-3
- EC Name:
- Methyl cyclopentylideneacetate
- Cas Number:
- 40203-73-4
- Molecular formula:
- C8H12O2
- IUPAC Name:
- methyl 2-cyclopentylideneacetate
- Test material form:
- other: liquid
- Details on test material:
- - Physical state: liquid
Constituent 1
Test animals
- Species:
- rat
- Strain:
- other: CD Strain, Crl: COBS CD (SD) BR
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: recognised animal supplier
- Weight at study initiation: male 154 - 266g; female 150 - 172g
- Fasting period before study: 24h before dosing
- Housing: housed in suspended wire-mesh stock cages
- Diet: standard laboratory diet ad libitum (except during the 24h period immediately prior to dosing)
- Water: ad libitum
- Acclimation period: at least 5 days
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- unchanged (no vehicle)
- Details on oral exposure:
- All doses were administered directly into the stomach of the rats using a hypodermic syringe equipped with a ball-tipped intubating needle.
- Doses:
- 600, 2025, 3038, 4556, 6834 mg/kg
- No. of animals per sex per dose:
- 2
- Control animals:
- no
- Details on study design:
- - Duration of observation period following administration: 14 days
- Frequency of observations and weighing: Initial and final body weights, mortalities and reactions were recorded.
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weights
Results and discussion
Effect levels
- Sex:
- male/female
- Dose descriptor:
- LD50
- Effect level:
- 3 362 mg/kg bw
- Based on:
- test mat.
- Remarks on result:
- other: calculated LD50
- Mortality:
- One animal from the 3038 mg/kg dose group died on day 2. All four animals from the 4556 mg/kg dose group died on day 1. All four animals from the 6834 mg/kg dose group died on day 1 and 2.
- Clinical signs:
- other: Clinical signs observed during the observation period include: hypoactivity, salivation, convulsions, muscular weakness, labored breathing, prostration, lacrimation, vasoconstriction (females), tremors, nasal discharge, exophthlmos (males), head motions
- Gross pathology:
- Necropsy examination of the animals that died revealed hemorrhaged lungs in animals 17-M, 18-M and 19-F. In addition, pale mottled livers were noted in animals 9-M, 11-F and 12-F; pale kidneys in animals 20-F, 9-M, 10-M, 11-F and 12-F; a distended gastrointestinal tract in animals 10-M and 11-F; gastric hemorrhages in animal 10-M; intestinal hemorrhages in animal 11-F and advanced postmortem autolysis (due to overnight mortality) in animal 16-F. Examination of the survivors revealed necrotic tissue in the stomach lining in animal 15-F.
Any other information on results incl. tables
Table 1. Mortality data
Dose level (mg/kg) | Animal number | Sex | Number dead/Number tested | % Dead |
600 | 1 | Male | 0/4 | 0 |
2 | Male | |||
3 | Female | |||
4 | Female | |||
2025 | 5 | Male | 0/4 | 0 |
6 | Male | |||
7 | Female | |||
8 | Female | |||
3038 | 13 | Male | 1/4 | 25 |
14 | Male | |||
15 | Female | |||
16 | Female | |||
4556 | 17 | Male | 4/4 | 100 |
18 | Male | |||
19 | Female | |||
20 | Female | |||
6834 | 9 | Male | 4/4 | 100 |
10 | Male | |||
11 | Female | |||
12 | Female |
Applicant's summary and conclusion
- Interpretation of results:
- sligthly toxic
- Remarks:
- Migrated information Criteria used for interpretation of results: US CPSC / US OSHA
- Conclusions:
- Under the conditions of this study, the LD50 of the test substance was determined to be 3362 mg/kg in the male/female rat.
- Executive summary:
The pre-GLP study was performed following a method similar to OECD 401 to assess the acute oral toxicity potential of the test substance to male/female CD Strain, Crl: COBS CD (SD) BR rats. The test material was administered as a single oral dose, by gavage, to a group of 10 male and 10 female rats following an overnight fast. The animals were observed for 14 days and survivors were then euthanized. All animals were subject to necropsy. One animal from the 3038 mg/kg dose group died on day 2; all remaining animals below this dose survived to the end of the study. Clinical signs of toxicity were noted in most animals on the day of dosing. There was no adverse effect on bodyweight gain in animals of either sex. Necropsy examination of the animals that died revealed haemorrhaged lungs in animals. In addition, pale mottled livers, pale kidneys, a distended gastrointestinal tract, gastric haemorrhages, intestinal haemorrhages and advanced postmortem autolysis (due to overnight mortality) were observed. Examination of the survivors revealed necrotic tissue in the stomach lining in one animal. Under the conditions of this study, the LD50 of the test substance was calculated to be 3362 mg/kg.
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