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EC number: 227-774-4 | CAS number: 5977-14-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: screening tests
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1989
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Test wa performed acc to DIN 38412 part 25, similar to OECD 302 B; no GLP
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 989
- Report date:
- 1989
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: DIN 38412 part 25
- Deviations:
- not specified
- GLP compliance:
- no
Test material
- Reference substance name:
- Acetoacetamide
- EC Number:
- 227-774-4
- EC Name:
- Acetoacetamide
- Cas Number:
- 5977-14-0
- Molecular formula:
- C4H7NO2
- IUPAC Name:
- acetoacetamide
Constituent 1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, industrial, non-adapted
- Details on inoculum:
- Mixture of activated sludge from the sewage treatment plant of the Hoechst AG at Frankfurt am Main (Höchst) with activated sludge fom the sewage treatmant plant at Hoechst AG site Kelsterbach in the ratio 30 : 70
- Duration of test (contact time):
- 28 d
Initial test substance concentration
- Initial conc.:
- 357.9 mg/L
- Based on:
- test mat.
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- DOC removal
- Details on study design:
- Stock solution: 5 g 30% acetoacetamide solution were mixed with 1800 ml de-ionized water, the pH adjusted from 6.9 to 7.1. This batch was stirred over a period of 24 hours at 22°C. The resulting clear solution was filled up with de-ionized water to a final volume of 2000 ml and used as stock solution.
Inoculum: Washing the sediment from the activated sludge two times by centrifuging and resuspending in mineral medium. 12 g/L (=1240 mg dry mass/L) was used for the batch.
241 ml/L stock solution was aded. Concentration: 357.9 mg/L
Reference substanceopen allclose all
- Reference substance:
- diethylene glycol
- Remarks:
- concentration: 271.6 mg DOC/L
- Reference substance:
- other: blank control: reaction medium with inoculum but without test or reference substance
Results and discussion
% Degradationopen allclose all
- Parameter:
- % degradation (DOC removal)
- Value:
- 32
- Sampling time:
- 1 d
- Parameter:
- % degradation (DOC removal)
- Value:
- 70
- Sampling time:
- 9 d
- Parameter:
- % degradation (DOC removal)
- Value:
- 68
- Sampling time:
- 19 d
- Parameter:
- % degradation (DOC removal)
- Value:
- 64
- Sampling time:
- 28 d
BOD5 / COD results
- Results with reference substance:
- Diethyleneglycol:
1 d 5%
9 d 82%
16 d 93% DOC removal
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- inherently biodegradable
- Conclusions:
- Acetoacetamide (30% solution) is inherently biodegradable under the conditions of the zahn-Wellens Test.
- Executive summary:
A 30% aequous solution of acetoacetamide was tested for inherent biodegradation in the Zahn-Wellens-test by measuring the DOC disappearance. During the first 7 days of the test the DOC exhaustion achieved > 70% of the carbon content of the added test substance, but the following day the rate of degradation staye at the plateau in the range 70 - 72%. The results were confirmed by additionally conducted COD control analyzes. COD at the start was 510 mg/L and 160 mg/L after 28 days, corresponding to an elimination of about 69%.
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