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EC number: 213-600-4 | CAS number: 993-00-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://echa.europa.eu/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_toxicological-information.png)
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- migrated information: read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Study period:
- 1987-06-03 to 1987-07-10
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: The study was conducted according to a test protocol that is comparable to the appropriate OECD test guideline. It was compliant with GLP. It is considered that read-across to the registered substance is scientifically justified.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 987
- Report date:
- 1987
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Principles of method if other than guideline:
- other: Ames et al. 1973
- GLP compliance:
- yes
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- Dichloro(dimethyl)silane
- EC Number:
- 200-901-0
- EC Name:
- Dichloro(dimethyl)silane
- Cas Number:
- 75-78-5
- IUPAC Name:
- dichloro(dimethyl)silane
Constituent 1
Method
Species / strain
- Species / strain / cell type:
- other: Salmonella typhimurium: TA 98, TA 100, TA 1535, TA1537, TA1538 and Escherichia coli WP2uvrA
- Metabolic activation:
- with and without
- Metabolic activation system:
- Aroclor induced rat liver S9
- Test concentrations with justification for top dose:
- See table 1.
- Vehicle / solvent:
- - Vehicle(s)/solvent(s) used: acetone
Controlsopen allclose all
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- sodium azide
- Remarks:
- TA 100, TA 1535 (without activation)
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- 9-aminoacridine
- Remarks:
- TA 1537 (without activation)
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- 2-nitrofluorene
- Remarks:
- TA 98, TA 1538 (without activation)
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- other: N-Methyl-N-nitro-N-nitrosoguanidine
- Remarks:
- WP2uvrA (without activation)
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- benzo(a)pyrene
- Remarks:
- All strains (with activation)
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- other: 2-aminoanthracene
- Remarks:
- All strains (with activation)
- Details on test system and experimental conditions:
- METHOD OF APPLICATION: in agar (plate incorporation)
NUMBER OF REPLICATIONS: 3 plates per test concentration
DETERMINATION OF CYTOTOXICITY
- Method: relative total growth; Background lawn assessment - Evaluation criteria:
- Mutagenicity of the test substance is indicated by an increase in the number of revertant colonies.
- Statistics:
- Statistical methods: Responses (numbers of revertants) to the test substance were compared to concurrent negative and positive
controls, as well as to historical data.
Results and discussion
Test results
- Species / strain:
- other: Salmonella typhimurium: TA 98, TA 100, TA 1535, TA1537, TA1538 and Escherichia coli WP2uvrA
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- other: >1500 ug/plate
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not applicable
- Positive controls validity:
- valid
- Additional information on results:
- The test compound was toxic to most of the bacterial strains and precipitate was observed at doses greater than or equal to 2500 ug/plate, both with and without metabolic activation. Final mutagenicity testing was conducted with 5000 ug/plate as the top dose. The results of the tests conducted on the test substance in the absence or presence of a metabolic activation system were all negative.
Revertants per plate for positive control substances ranged from 18- 680, depending on the agent and strain. - Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Any other information on results incl. tables
Table 2 : Exp 1 : Mutagenicity assay for all strains with and without metabolic activation (mean of 3 plates)
|
TA98 |
TA100 |
TA1535 |
||||||
Conc. |
— MA |
+ MA |
Cytotoxic |
— MA |
+ MA |
Cytotoxic |
— MA |
+ MA |
Cytotoxic |
0* |
25 |
30 |
No |
158 |
175 |
No |
13 |
12 |
No |
4 |
21 |
29 |
No |
153 |
159 |
No |
12 |
11 |
No |
20 |
19 |
28 |
No |
148 |
174 |
No |
11 |
12 |
No |
100 |
23 |
28 |
No |
152 |
181 |
No |
12 |
10 |
No |
500 |
27 |
24 |
No |
148 |
187 |
No |
14 |
12 |
No |
2500 |
18 |
23 |
No |
166 |
174 |
No |
8 |
14 |
No |
10,000 |
- |
3 |
Yes |
- |
- |
Yes |
6 |
2 |
Yes |
Positive Control |
364 |
437 |
No |
554 |
611 |
No |
404 |
119 |
No |
*solvent control with Acetone
Table 2 : Exp 1 : Mutagenicity assay for all strains with and without metabolic activation (mean of 3 plates)
|
TA1537 |
TA1538 |
WP2uvrA |
||||||
Conc. |
— MA |
+ MA |
Cytotoxic |
— MA |
+ MA |
Cytotoxic |
— MA |
+ MA |
Cytotoxic |
0* |
12 |
8 |
No |
17 |
17 |
No |
47 |
52 |
No |
4 |
9 |
8 |
No |
16 |
20 |
No |
43 |
51 |
No |
20 |
7 |
8 |
No |
13 |
20 |
No |
44 |
53 |
No |
100 |
7 |
9 |
No |
16 |
22 |
No |
40 |
48 |
No |
500 |
8 |
5 |
No |
16 |
24 |
No |
46 |
57 |
No |
2500 |
8 |
6 |
No |
16 |
11 |
No |
43 |
41 |
No |
10,000 |
- |
0 |
Yes |
4 |
1 |
Yes |
- |
12 |
Yes |
Positive Control |
155 |
84 |
No |
591 |
547 |
No |
241 |
174 |
No |
*solvent control with Acetone
Table 3 : Exp 2 : Repeat Assay Number of revertants per plate (mean of 3 plates)
|
TA98 |
TA100 |
TA1535 |
||||||
Conc. |
— MA |
+ MA |
Cytotoxic |
— MA |
+ MA |
Cytotoxic |
— MA |
+ MA |
Cytotoxic |
0* |
19 |
32 |
No |
168 |
205 |
No |
16 |
13 |
No |
4 |
27 |
40 |
No |
153 |
234 |
No |
17 |
13 |
No |
20 |
23 |
36 |
No |
180 |
2335 |
No |
17 |
13 |
No |
100 |
26 |
35 |
No |
182 |
197 |
No |
15 |
12 |
No |
500 |
24 |
27 |
No |
141 |
207 |
No |
11 |
16 |
No |
2500 |
24 |
29 |
No |
183 |
207 |
No |
16 |
16 |
No |
5000 |
- |
25 |
Yes |
119 |
167 |
No |
13 |
16 |
No |
Positive control |
249 |
377 |
No |
483 |
628 |
No |
308 |
76 |
No |
*solvent control with Acetone
Table 3 : Exp 2 : Repeat Assay Number of revertants per plate (mean of 3 plates)
|
TA1537 |
TA1538 |
WP2uvrA |
||||||
Conc. |
— MA |
+ MA |
Cytotoxic |
— MA |
+ MA |
Cytotoxic |
— MA |
+ MA |
Cytotoxic |
0* |
12 |
9 |
No |
14 |
23 |
No |
48 |
75 |
No |
4 |
8 |
9 |
No |
13 |
26 |
No |
61 |
68 |
No |
20 |
9 |
8 |
No |
13 |
26 |
No |
59 |
58 |
No |
100 |
9 |
7 |
No |
15 |
20 |
No |
63 |
52 |
No |
500 |
8 |
7 |
No |
12 |
23 |
No |
50 |
35 |
No |
2500 |
9 |
10 |
No |
16 |
19 |
No |
39 |
37 |
No |
5000 |
4 |
6 |
Yes |
14 |
18 |
No |
23 |
26 |
Yes |
Positive control |
94 |
91 |
No |
458 |
515 |
No |
365 |
304 |
No |
*solvent control with Acetone
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
negative with metabolic activation
negative without metabolic activation
Under test conditions, dimethyldichlorosilane did not demonstrate genetic activity in any of the tests, with or without metabolic activation. The results indicate that the test substance was not considered mutagenic.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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