1,2-benzisothiazol-3(2H)-one 1,1-dioxide

Endpoint summary

Additional information

Summary of Acutic tox results:

Short term toxicity to fish:

No. of studies reviewed for Short-term toxicity to fish from reliable sources having Klimisch rating 2.

The summary of the results are presented below:

 

Sr.No	Endpoint	Effect values	Basis for effects	Duration	Species	CAS No.	Sources
1	LC50	18300 mg/L	Mortality	96 hrs.	Pimephales promelas	81-07-2	Experimental data
2	NOEC	2500 mg/L	Behaviour	24 - 26 hours post fertilization	Danio rerio	81-07-2	Experimental data from publication
3	LC50	7272.4 mg/L	Development	24 to 72 hour(s)	Danio rerio	81-07-2	Experimental data from publication
4	LC50	293.411 mg/L	Mortality	96 hrs.	Leuciscus idus	81-07-2	Predicted data from QSAR
5	LC50	5585.3 mg/L	Development	144 hrs.(6 days)	Danio rerio	81-07-2	Experimental data from publication
6	EC50	4755.5mg/L	Development	144 hrs.(6 days)	Danio rerio	81-07-2	Experimental data from publication
7	LC50	>100 mg/L	Mortality	24-96 hrs.	Poecilia reticulata	Read across 128-44-9	Experimental data

 

By applying weight of evidence approach to the target substance 1,2-benzisothiazol-3(2H)-one 1,1-dioxide and the read across substance 1,2-benzisothiazol-3(2H)-one 1,1-dioxide, sodium salt (which have more than 87.8% structural similarity),the endpoint value was found to vary between LC50 = >100 mg/L to 18300 mg/L in a 24 hrs.study to 96 hrs.study on the basis of mortality effect,EC50=4755.5mg/L basis of Development effect and NOEC for24 - 26 hours post fertilization period was reported as 2500 mg/L

 

Thus considering the CLP Criteria for aquatic classification of the substance, it is concluded that the test substance 1,2-benzisothiazol-3(2H)-one 1,1-dioxide does not exhibits short term toxicity to fish (Leuciscus idus, Danio rerio ,Pimephales promelas and Poecilia reticulata).

 

Long term toxicity to fish:

In a long term toxicity study, 451 Danio sp. (Danio) Zebrafish embryo(s) were treated with Saccharin.

Embryo(s) and larva were observed for highest non-teratogenic concentration (HNTC), spontaneous tail coilings in zebra fish embryos and eleutheroembryonic and larval locomotor activity for 144 hours post fertilization.

No effect were observed on highest non-teratogenic concentration (HNTC), spontaneous tail coilings in zebra fish embryos and eleutheroembryonic and larval locomotor activityfor 144 hours post fertilizationofEmbryo(s) and larva of treated fish as compared to control.

Therefore, NOEC was considered to be 2500 mg/l when 451 Danio sp. (Danio) Zebrafish embryo(s) treated with Saccharin for 144 hours post fertilization.

Short term toxicity to aquatic invertebrates

The effective concentration (EC50) value of 1,2-benzisothiazol-3(2H)-one 1,1-dioxide in aquatic invertebrate (Daphnia pulex) in a 48 hr study on the basis of intoxication effect was estimated to be 104.403 mg/L.Thus,considering the CLP Criteria for aquatic classification of the substance , it is concluded that 1,2-benzisothiazol-3(2H)-one 1,1-dioxide does not exhibit short term toxicity to aquatic invertebrate (Daphnia pulex).

Long-term toxicity to aquatic invertebrates

According to annex IX of column I ; Long-term toxicity testing on invertebrate (species : Daphnia magna) need not to be conducted as it is already provided as a part of Annex VII requirements.Therefore this end point was considered for waiver.

 

Toxicity to aquatic algae and cyanobacteria

After 72 hours of exposure to 1,2-benzisothiazol-3(2H)-one 1,1-dioxide (CAS No. 81-07-2) to various nominal test concentration, EC50 was found to be 541170mg/L (>200mg/l with an R square of 0.961) and EC25 was found to be 569.581 mg/L (with an R square of 0.961).

Toxicity to microorganisms

The effective concentration (EC50) value of 1,2-benzisothiazol-3(2H)-one 1,1-dioxide in microorganism (Tetrahymena thermophila) in a 48 hr study on the basis of population effect was estimated to be 123.04 mg/L.