2,6,6-trimethylbicyclo[3.1.1]heptane-3-carbaldehyde

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: non-GLP and non-guideline study, but well documented study report

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1985

Report date:

1985

Materials and methods

Principles of method if other than guideline:

according to BASF-internal standard

GLP compliance:

no

Test type:

standard acute method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

2,6,6-trimethylbicyclo[3.1.1]heptane-3-carbaldehyde

EC Number:

262-062-7

EC Name:

2,6,6-trimethylbicyclo[3.1.1]heptane-3-carbaldehyde

Cas Number:

60113-43-1

Molecular formula:

C11H18O

IUPAC Name:

2,6,6-trimethylbicyclo[3.1.1]heptane-3-carbaldehyde

Details on test material:

Name of the test substance used in the study report: Formylpinan R

Test animals

Species:

rat

Strain:

Wistar

Sex:

male/female

Details on test animals or test system and environmental conditions:

Five animals per cage (type: stainless steel wire mesh cages) were housed in fully air-conditioned rooms with a temperature in the range of 20 - 24°C and a relative humidity of 30 - 70%. The day/night rhythm was 12 h dark and 12 h light. A standard animal laboratory diet and tap water were available ad libitum.

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

olive oil

Details on oral exposure:

Only animals with comparable weights were used.
The animals were given no feed 16 hours before administration (water was available ad libitum).
Because the test substance was sensitive to hydrolysis, olive oil was used.

Doses:

1000, 1470, 2150, 3160 mg/kg

No. of animals per sex per dose:

5

Control animals:

no

Details on study design:

Duration of observation period following administration: 14 days
Recording of signs and symptoms were done several times on the day of administration (at least once each working day). A check for moribund or dead animals were made twice each working day and once on holidays.
Withdrawal of food 16 hours before sacrifice with CO2; then necropsy with gross-pathological examination. Necropsy of all animals that die as soon as possible.

Results and discussion

Effect levels

Effect levels 1

Sex:

male/female

Dose descriptor:

LD50

Effect level:

2 000 mg/kg bw

Effect levels 2

Sex:

female

Dose descriptor:

LD50

Effect level:

ca. 1 470 mg/kg bw

Effect levels 3

Sex:

male

Dose descriptor:

LD50

Effect level:

ca. 2 850 mg/kg bw

Remarks on result:

other: value from interpolation

Mortality:

Male animals:
1000, 147, 2150 mg/kg: no deaths
3160 mg/kg: 4/5 after 14 days

Female animals:
1000 and 1470 mg/kg: no deaths
2150 mg/kg: 5/5 after 14 days
3160 mg/kg: 5/5 after 14 days

Clinical signs:

other: Both sexes: dyspnea, apathy, abnormal position, staggering atonia, paresis, pain relfex absent, corenal reflex absent, narcotic-like state, twitching, piloerection, diarrhea, erythema, exsiccosis, imbalance, poor general state. Additional signs for female

Gross pathology:

Animals that died (males and females): general congestive hyperemia. Stomach: dilated, filled with feed and liquid contents.
Sacrificed animals (males and females): no abnormalities detected.