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EC number: 939-479-4 | CAS number: 1471311-60-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Endpoint summary
Administrative data
Description of key information
Two in vitro tests were performed in order to assess the skin corrosive/irritating potential of LAS MIPA. Both studies gave a negative result, and hence the substance is not irritating to the skin. The in vitro eye irritating test suggests that the substance is irritating to the eye. However, since the test is not so far officially accepted for regulatory purposes, it is proposed that the test material is classified as damaging to the eye.
Key value for chemical safety assessment
Skin irritation / corrosion
Endpoint conclusion
- Endpoint conclusion:
- no adverse effect observed (not irritating)
Eye irritation
Link to relevant study records
- Endpoint:
- eye irritation: in vitro / ex vivo
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- October 2012
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: GLP study. No specific guideline followed.
- Qualifier:
- no guideline available
- Principles of method if other than guideline:
- The test is based on the hypothesis that irritant chemicals are able to penetrate the corneal epithelial tissue and are sufficiently cytotoxic to cause cell death. Cytotoxicity is measured with the MTT reduction assay.
- GLP compliance:
- yes (incl. QA statement)
- Species:
- other: not applicable
- Details on test animals or tissues and environmental conditions:
- SkinEthic reconstructed Human Corneal Epithelium model (HCE, SkinEthic Laboratories, Lyon, France)
- Vehicle:
- unchanged (no vehicle)
- Controls:
- other: not applicable
- Amount / concentration applied:
- see below details on study design
details on negative control (Solution A); compostition for 1 L: Na2HPO4 0.142 g/l, glucose 1.802 g/l, HEPES 7.149 g/l, KCl 0.224 g/l and NaCl 7.597 g/l
positive control: sodium dodecyl sulphate 2% w/v - Details on study design:
- Pre-test: Test MTT reduction by the test material: 30 ul test material + 1 ml 0.5 mg/ml MTT solution incubated for 3 h. The blue colour indicates reduced MTT.
MAIN TEST
7-day old tissues were transferred into wells and treated with 30 ul test item
Exposure period: 10 min
Negative control: 30 ul solution A
Positive control: 30 ul of 2% w/v SDS
Washing: DPBS without Ca++ and Mg++
Placement of each tissue into a 24-well plate with 300 ul maintenance medium
MTT loading plates: 300 ul of a 0.5 mg/ml MTT solution
Incubation: 3 hours
Washing: phosphate buffered saline
Isopropanol was applied for the formation of formazan crystals
Optical density (OD) was measured at 540 nm with Anthos 2001 microplate reader
Tissue histopathology: one tissue/treatment group was kept for histopathological examination - Remarks on result:
- not measured/tested
- Other effects / acceptance of results:
- The MTT solution conatining the test item did not turn blue, suggesting that the test material does not reduce directly MTT.
- Interpretation of results:
- irritating
- Remarks:
- Migrated information irritating Criteria used for interpretation of results: other: criteria of the test
- Conclusions:
- Based on the results of the study, Marlon AMI 80 and hence, benzenesulfonic acid, 4-C10-13-sec-alkyl derivs.-, compd. with isopropanolamine (1:1), are both eye irritants.
- Executive summary:
The purpose of this study was to determine the eye irritation potential of Marlon AMI 80 using the SkinEthic reconstructed Human Corneal Epithelium model (HCE, SkinEthic Laboratories, Lyon, France) after a treatment period of 10 minutes. The test is based on the hypothesis that irritant chemicals are able to penetrate the corneal epithelial tissue and are sufficiently cytotoxic to cause cell death. Cytotoxicity is measured with the MTT reduction assay.
Triplicate tissues were exposed to the test material and thereafter, to MTT. The optical density was measured at 540 nm.Data were presented in the form of percentage viability (MTT conversion relative to negative controls).The relative mean viability of the test item treated tissues after a 10 min exposure period was 8.3%, i.e. the test the test item is an eye irritant.
Reference
Table 1: Assessment of Eye Irritation Potential- Viability of HCE tissues
Item |
OD540of Individual Tissue |
Mean OD540 |
Relative Mean Viability (%) |
Negative Control |
0.727 |
0.698 |
100 |
0.669 |
|||
Positive Control |
0.019 |
0.026 |
3.7 |
0.033 |
|||
Test item |
0.058 |
0.058 |
8.3 |
0.057 |
Tissue histopathological examination was not performed cause it was not considered necessary.
Endpoint conclusion
- Endpoint conclusion:
- adverse effect observed (irritating)
Respiratory irritation
Endpoint conclusion
- Endpoint conclusion:
- no study available
Additional information
Skin irritation
The potential skin effects of MARLON AMI 80 (78% benzenesulfonic acid, 4-C10-13-sec-alkyl derivs.-, compd. with isopropanolamine (1:1) in 1,2-propylene glycol) were tested in two in vitro studies with the use of reconstructed human epidermis models (EPISKINTM). The principle of the assays is based on the measurement of cytotoxicity in the human epidermal cultures following topical exposure to the test material, with the MTT reduction assay. Both test gave negative results, suggesting that MARLON AMI 80, and hence, LAS MIPA, has no skin corrosive or irritating properties.
Eye irritation
An in vitro eye irritation test was performed with the use of the SkinEthic Human Corneal Epithelium model. Currently, the aforementioned test is not accepted for regulatory purposes. Therefore, the classification of the test item cannot be based solely on this test. Since no additional information is available on the eye irritation potential of benzenesulfonic acid, 4-C10-13-sec-alkyl derivs.-, compd. with isopropanolamine (1:1), the substance shall be classified and labelled as corrosive to the eye.
Justification for selection of skin irritation / corrosion endpoint:
Weight of evidence approach applied
Justification for selection of eye irritation endpoint:
Only one study available
Effects on eye irritation: corrosive
Justification for classification or non-classification
According to the classification criteria laid down on EC Regulation 1272/2008, LAS MIPA shall not be classified for skin irritation. The in vitro eye irritation test suggests that the substance is irritating to the eye. However, hitherto the test is not officially accepted for regulatory purposes, and hence, it is proposed that LAS MIPA is classified as damaging to the eye, Eye Dam.1, H318.
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