Potassium trifluoroacetate

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

From 18 jan 2000 to 20 jan 2000

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Qualifier:

equivalent or similar to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

no

Analytical monitoring:

no

Vehicle:

no

Details on test solutions:

The stock solution was prepared by dissolving the test substance directly in the test medium at a concentration of 100 mg/kg (100 mg/L).
Tests solutions were prepared by dilution of the stock solution to obtain the appropriate test concentrations as follow: 0.1, 0.35, 1, 3.5, 10, 35 and 100 mg/L.

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Daphnia magna
- Strain: Strauss 1820
- Source: Laboratory CRIT-D, Décines
No more data

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test temperature:

18 - 22°C (variability: +/- 1°C)

Nominal and measured concentrations:

0, 0.1, 0.35, 1, 3.5, 10, 35 and 100 mg/L (nominal).

Details on test conditions:

TEST SYSTEM
- Test vessel: 100 mL beaker
- Type (delete if not applicable): open
- Aeration: no data
- No. of organisms per vessel: 20
- No. of vessels per concentration (replicates): 1 except for the 100 mg/L dose, in duplicate
- No. of vessels per control (replicates): 1

TEST MEDIUM / WATER PARAMETERS
- CaCl2, 2H2O: 0.294 g
- MgSO4, 7H2O: 0.12325 g
- NaHCO3: 0.06475 g
- KCl: 0.00575 g
- Bidistilled water: up to 1000 mL

OTHER TEST CONDITIONS
- Adjustment of pH: no data
- Photoperiod: none, darkness
- Light intensity: darkness

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : mortality, immobilization.

TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2.8 or 3.5
- Test concentrations: 0, 0.1, 0.35, 1, 3.5, 10, 35 and 100 mg/L

Reference substance (positive control):

yes

Remarks:

Potassium dichromate

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Details on results:

No mortality has been observed during the study.

Results with reference substance (positive control):

- Results with reference substance valid: yes
- EC50 (24h): 0.91 mg/L (CL: 0.76 - 1.2 mg/L)

Reported statistics and error estimates:

Probit method for reference substance.

Validity criteria fulfilled:

yes

Remarks:

<10% f mortality in controls was fulfilled; Oxygen demand >= 3mg/L: no data

Conclusions:

Potassium trifluoroacetate is not harmful to daphnia

Executive summary:

In this key study, Daphnia magna were exposed to Potassium trifluoroacetate at doses from 0 to 100 mg/L, following the EC C2 guideline. There was no mortality observed during the study.  The 48-hour EC50 was higher than 100 mg/L. In these conditions, Potassium trifluoroacetate is not harmful to daphnia based on CLP 1272/2008 criteria. 

Description of key information

No effect of TFAK was observed to Daphnia magna at 100 mg/L.

Additional information

A reliable key study is available for this endpoint. In this key study, Daphnia magna were exposed to Potassium trifluoroacetate at doses from 0 to 100 mg/L, following the EC C2 guideline. There was no mortality observed during the study. The 48-hour EC50 was higher than 100 mg/L. Under these conditions, Potassium trifluoroacetate is not harmful to daphnia based on EU criteria (CLP 1272/2008).

Moreover, a reliable key study is available for sodium trifluoroacetate which is used in a read-across approach. In this supporting study, the acute toxicity of sodium trifluoroacetate to water fleas Daphnia magna (0-24 hours old) was studied in a limit test according OECD Guideline 202 (OECD, 1984) at nominal test concentrations of 0 and 1200 mg/l in ISO-water. Samples of the test solutions were taken 0 and 48 hours after test initiation and analysed by ion chromatography. The concentration of sodium trifluoroacetate remained constant during the test. No immobilization was observed during the test in any test concentration and no other effects were observed. Therefore, based on the nominal test concentrations, for sodium trifluoroacetate, the EC50 (48h) is > 1200 mg/L.