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EC number: 270-279-3 | CAS number: 68424-19-1 This substance is identified by SDA Substance Name: C16-C18 and C18 unsaturated alkyl carboxylic acid triethanol amine salt and SDA Reporting Number: 11-006-14.
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2004
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Remarks:
- Guideline compliant study with good documentation
- Qualifier:
- according to guideline
- Guideline:
- other: ISO 14669: Determination of acute lethal toxicity to marine copepods (Copepods, Crustacea)
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Details on sampling:
- Samples of the water accommodated fractions with loading 100 and 1000 mg/L and the control medium were taken at the start of the test and analysed for content of total organic carbon (TOC).
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The test substance is a multi-component substance of low water solubility. Therefore the test was performed on water accommodated fractions (WAF) as recommended by OECD. Separate WAFs were prepared for 5 loading rates from 100 to 1000 mg/l. The test substance was transferred to 0.5 l of the test medium in 1 l sealed glass bottles. The flasks were placed on a reciprocating shaker. The WAF extraction was performed in the darkness in a room controlled at 21±1 °C. After 22 hours, the agitation was terminated and approximately two hours later, 200 ml of each WAF was drawn with a pipette, avoiding the settled material at the bottom of the flasks. All WAFs with loadings were slightly turbid and were filtered through Whatmen GF/C fibre filters to remove non settling suspended material. - Test organisms (species):
- other aquatic crustacea: Acartia tonsa
- Details on test organisms:
- TEST ORGANISM
- Common name: Acartia tonsa
- Source: Marine Research Laboratory, Helsingör, Denmark
- Age at study initiation (mean and range, SD): 17- 30 days
Cultured in natural seawater; fed with Nephroselmis pyriformis and Rhodomonas baltica - Test type:
- static
- Water media type:
- saltwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Test temperature:
- Preparation of WAF: 21.0 - 21.6°C
Test temperature: 20.1 - 20.3°C - pH:
- 7.8 - 8.1
- Dissolved oxygen:
- 7.1 - 7.3 mg/L
- Salinity:
- 32 S
- Nominal and measured concentrations:
- Nominal concentrations: Control, 100, 180, 320, 560 and 1000 mg/L, measured concentrations in the control and 100 and 1000 mg/L test solutions were 0.95, 6.7 and 40.4 mg TOC/L.The results indicate that less than 10 % of the test substance partitioned to the water phase as dissolved organic matter. (The estimate is based on the assumption that the carbon content of the test substance is 70% by weight).
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 50 mL polysterene beakers
- No. of organisms per vessel: 5 - 8
- No. of vessels per concentration (replicates): 16
- No. of vessels per control (replicates): 4
- No. of vessels per vehicle control (replicates): none
- Biomass loading rate: NA
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: natural seawater, taken at 60 depth at Solbergstrand Research Station in outer Oslo Fjord, The salinity was adjusted to 32 S using destilled water
- Intervals of water quality measurement: pH at start and end, DO at end of the exposure.
OTHER TEST CONDITIONS
- Adjustment of pH: not reported
- Photoperiod: in the dark
- Light intensity: in the dark
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : mortality
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 1.8
- Justification for using less concentrations than requested by guideline: NA
- Range finding study: not reported - Reference substance (positive control):
- yes
- Remarks:
- 3,5 dichlorophenol
- Duration:
- 48 h
- Dose descriptor:
- LL50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Details on results:
- - Behavioural abnormalities: Not assessed
- Observations on body length and weight: Not assessed
- Other biological observations: Mortality
- Mortality of control: 1 %
- Other adverse effects control: 1 out to 146 animals
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: Not reported for the final test solutions
- Effect concentrations exceeding solubility of substance in test medium: Yes (WAF) - Results with reference substance (positive control):
- - Results with reference substance valid? Yes
- Mortality: 30 % at 1 mg/L, validity criterion: Within 20 - 80%) - Reported statistics and error estimates:
- Not calculated since less than 50 % mortality in any of the test solutions.
- Validity criteria fulfilled:
- yes
- Executive summary:
In the Klimisch 1 study from Sverinsen (2004) the acute toxicity of the test material (Triethanolamine salt of oleic acid) to the marine crustacean Acartia tonsa was determined in a 48 h static GLP acute lethal toxicity to marine copepods. The test design was based on ISO 14669: "Determination of acute lethal toxicity to marine copepods (Copepods, Crustacea)".
Since the test substance is a UVCB substance of low water solubility the test was performed on water accommodated fractions (WAF) as recommended by OECD. The nominal loading rates were 0 (control), 100, 180, 320, 560 and 1000 mg test item/L. The WAF extraction was performed in the darkness in a room controlled at 21±1 °C. After 22 hours, the agitation was terminated and approximately two hours later, 200 ml of each WAF was drawn with a pipette, avoiding the settled material at the bottom of the flasks. All WAFs with loadings were slightly turbid and were filtered through Whatmen GF/C fibre filters to remove non settling suspended material.
Sixteen control replicates and 4 replicates from each test solution were set up. Each replicate received 5 - 8 animals. Dose verification analysis was performed by TOC analysis of the control, 100 and 1000mg/L test solutions at the start of the test. The test was performed in darkness. A test with a reference substance gave a valid result.
Since only minor mortality was observed resulting in < 10% mortality in any of the tested WAFs the highest loading tested resulting in 50% mortality (LL50) is >1000 mg/l.
This study is considered to be acceptable for the risk assessment.
Reference
Number of alive Acartia tonsa after 24 and 48 hours
Loading (mg test item/L) |
Number of aliveAcartia tonsa |
Percent alive after 48 hours |
||
Start |
24 h |
48 h |
||
0 (control) |
146 |
146 |
145 |
99 |
100 |
22 |
21 |
20 |
91 |
180 |
26 |
26 |
26 |
100 |
320 |
29 |
29 |
28 |
97 |
560 |
26 |
25 |
25 |
96 |
1000 |
26 |
25 |
25 |
96 |
Description of key information
48h-LL50: > 1000 mg/L
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 1 000 mg/L
Additional information
In the Klimisch 1 study from Sverinsen (2004) the acute toxicity of the test material (Triethanolamine salt of oleic acid) to the marine crustacean Acartia tonsa was determined in a 48 h static GLP acute lethal toxicity to marine copepods. The test design was based on ISO 14669: "Determination of acute lethal toxicity to marine copepods (Copepods, Crustacea)".
Since the test substance is a UVCB substance of low water solubility the test was performed on water accommodated fractions (WAF) as recommended by OECD. The nominal loading rates were 0 (control), 100, 180, 320, 560 and 1000 mg test item/L. The WAF extraction was performed in the darkness in a room controlled at 21±1 °C. After 22 hours, the agitation was terminated and approximately two hours later, 200 ml of each WAF was drawn with a pipette, avoiding the settled material at the bottom of the flasks. All WAFs with loadings were slightly turbid and were filtered through Whatmen GF/C fibre filters to remove non settling suspended material.
Sixteen control replicates and 4 replicates from each test solution were set up. Each replicate received 5 - 8 animals. Dose verification analysis was performed by TOC analysis of the control, 100 and 1000mg/L test solutions at the start of the test. The test was performed in darkness. A test with a reference substance gave a valid result.
Since only minor mortality was observed resulting in < 10% mortality in any of the tested WAFs the highest loading tested resulting in 50% mortality (LL50) is >1000 mg/l.
This study is considered to be acceptable for the risk assessment.
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