Sodium bis[2-chloro-4-[(4,5-dihydro-3-methyl-5-oxo-1-phenyl-1H-pyrazol-4-yl)azo]-5-hydroxybenzenesulphonamidato(2-)]chromate(1-)

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1977

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study with acceptable restrictions

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Version / remarks:

Inhouse SOP (method A.11) validated by Ciba which is comparable to standard guideline.

Deviations:

not specified

Principles of method if other than guideline:

In principle the methods described in OECD Guideline 203 were used (see any other information on materials and methods).

GLP compliance:

no

Analytical monitoring:

no

Vehicle:

not specified

Test organisms (species):

other: Trout

Details on test organisms:

8 trouts per concentration were used to performed the experiment.

Test type:

not specified

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test temperature:

14 °C

pH:

8-8.2

Nominal and measured concentrations:

2, 20 and 100 mg/L.

Reference substance (positive control):

no

Duration:

48 h

Dose descriptor:

LC0

Effect conc.:

2 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

8 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

48 h

Dose descriptor:

LC100

Effect conc.:

16 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

-At 2 mg/l: All fishes were alive at 24 and 48 h, (pH=8 at 48 h)
-At 20 mg/l: All fishes died at 24 h, (pH=8.1 at 48 h)
-At 100 mg/l: All fishes died at 24 h, (pH=8.2 at 48 h)

Conclusions:

The 48 h LC50 of FAT 20028/B in trout fish is 8 mg/L.

Executive summary:

The acute fish toxicity study experiment was carried out in 8 Trout fishes according to the method (A.11) comparable to OECD 203 guideline. During the test, the pH was between 8 -8.2 while temperature 14°C.

At concentration 2 mg/l of FAT 20028/A all fishes were alive at 24 h and 48 h. At 20 and 100mg/L, all fishes died at exposure 24 h.

Based on the study results, the LC₅₀of FAT 20028/A in trout is 8 mg/L, while LC₀(48h) is 2 mg/L and LC₁₀₀(48 h) is 16 mg/L.

Description of key information

The acute fish toxicity studies were performed with Acid Red 211 in trout and zebra fish. In a key study with FAT 20028/B the LC₅₀of FAT 20028/A in trout is 8 mg/L, while LC₀(48h) is 2 mg/L and LC₁₀₀(48 h) is 16 mg/L. In few supporting studies the LC₅₀was found as,

 

The LC₅₀of FAT 20028/A in trout is 6.3 mg/L, LC₀(48h) is 2 mg/L and LC₁₀₀(48 h) is 20 mg/L.

 

The LC₅₀of FAT 20028/C in trout is 7 mg/L (48 h exposure) and is 6 mg/l after 96 h exposure. While, LC₀(48h) is 3 mg/L and LC₁₀₀(48 h) is 30 mg/L.

The value taken forward for the risk assessment is 8 mg/L identified in the key study.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

8 mg/L